Biochemical, structural characterization and in-vitro evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of Cinnamomum zeylanicum bark extract

Nawaz, Aqsa; Ali, Tanvir; Naeem, Muhammad Asif; Hussain, Fatma; Li, Zhiye; Nasır, Abdul

doi:10.3389/fchem.2023.1194389

Cited by 4 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optical density (OD) was then assessed using a twin-beam UV/Visible spectrophotometer at about 510 nm. Finally, using the calibration curve for catechin, the total contents of flavonoid in the nanosuspension and extract was determined [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach

Raza,

Ali,

Naeem

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Swertia chirayita is used as a traditional medicinal plant due to its pharmacological activities, including antioxidant, antidiabetic, antimicrobial, and cytotoxic. This study was aimed to evaluate the therapeutic efficacy of newly synthesized nanosuspensions from Swertia chirayita through nanotechnology for enhanced bioactivities. Biochemical characterization was carried out through spectroscopic analyses of HPLC and FTIR. Results revealed that extract contained higher TPCs (569.6 ± 7.8 mg GAE/100 g)) and TFCs (368.5 ± 9.39 mg CE/100 g) than S. chirayita nanosuspension, TPCs (500.6 ± 7.8 500.6 ± 7.8 mg GAE/100 g) and TFCs (229.5± 3.85 mg CE/100 g). Antioxidant activity was evaluated through DPPH scavenging assay, and nanosuspension exhibited a lower DPPH free radical scavenging potential (06 ±3.61) than extract (28.9± 3.85). Anti-dabetic potential was assessed throughα-amylase inhibition and anti-glycation assays. Extract showed higher (41.4%) antiglycation potential than 35.85% nanosuspension and 19.5% α-amylase inhibitory potential than 5% nanosuspension. Biofilm inhibition activity against E. coli was higher in nanosuspension (69.12%) than extract (62.08%). The extract showed high cytotoxicity potential (51.86%) than nanosuspension (33.63%). These nanosuspensions possessed enhanced bioactivities for therapeutic applications could be explored further for the development of new drugs.

show abstract

Section: Methodsmentioning

confidence: 99%

Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach

Raza,

Ali,

Naeem

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

Enhanced Antibacterial and Antibiofilm Activities of Actinobacterial Therapeutic Metabolites Mediated Nanosuspension

Singh,

Sharma,

Dubey

2024

Part & Part Syst Charact

View full text Add to dashboard Cite

The escalation in infections from World Health Organization (WHO)‐listed priority pathogens has made development of new antibacterial agents a critical priority. In this context, use of therapeutic secondary metabolites (SMs) from Actinobacteria as new drugs presents a promising avenue. However, majority of them fail to reach market due to low aqueous solubility and hence low bioavailability. Even though nanosuspension technology has been effectively used to address these challenges, use of this technology for nanox02010;listed priority pathogens has made development of new antibacterial agents a critical priority. In thitransformation of crude metabolites from Actinobacteria is still an unattempted area. Herein, for the first time, development of water‐soluble nanosuspension of water‐insoluble therapeutic metabolites produced by Streptomyces californicus strain ADR1 to develop a biocompatible material to be used as potential nanomedicine is reported. The nanosuspension (N‐SM) is characterized by UV‐vis spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and dynamic light scattering (DLS). The nanosuspension reduces the MIC values by 50% against Gram‐positive priority pathogens and eradicates established biofilms with fivefold efficiency incomparison to SMs. The nanosuspension also displays antioxidant activity. The findings open up future possibilities of using this novel nanosuspension as an effective antibacterial agent in various therapeutic and biomedical applications like wound dressings, coatings on medical equipment, and surgical implants.

show abstract

Autophagy: A New Avenue and Biochemical Mechanisms to Mitigate the Climate Change

Azmat,

Zaheer,

Shaban

et al. 2024

Scientifica

View full text Add to dashboard Cite

Autophagy is a preserved process in eukaryotes that allows large material degeneration and nutrient recovery via vacuoles or lysosomes in cytoplasm. Autophagy starts from the moment of induction during the formation of a phagophore. Degradation may occur in the autophagosomes even without fusion with lysosome or vacuole, particularly in microautophagosomes. This process is arbitrated by the conserved machinery of basic autophagy‐related genes (ATGs). In selective autophagy, specific materials are recruited by autophagosomes via receptors. Selective autophagy targets a vast variety of cellular components for degradation, i.e., old or damaged organelles, aggregates, and inactive or misfolded proteins. In optimal conditions, autophagy in plants ensures cellular homeostasis, proper plant growth, and fitness. Moreover, autophagy is essential during stress responses in plants and aids in survival of plants. Several biotic and abiotic stresses, i.e., pathogen infection, nutrient deficiency, plant senescence, heat stress, drought, osmotic stress, and hypoxia induce autophagy in plants. Cell death is not a stress, which induces autophagy but in contrast, sometimes it is a consequence of autophagy. In this way, autophagy plays a vital role in plant survival during harsh environmental conditions by maintaining nutrient concentration through elimination of useless cellular components. This review discussed the recent advances regarding regulatory functions of autophagy under normal and stressful conditions in plants and suggests future prospects in mitigating climate change. Autophagy in plants offers a viable way to increase plant resilience to climate change by increasing stress tolerance and nutrient usage efficiency.

show abstract

Biochemical, structural characterization and in-vitro evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of Cinnamomum zeylanicum bark extract

Cited by 4 publications

References 39 publications

Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach

Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach

Enhanced Antibacterial and Antibiofilm Activities of Actinobacterial Therapeutic Metabolites Mediated Nanosuspension

Autophagy: A New Avenue and Biochemical Mechanisms to Mitigate the Climate Change

Contact Info

Product

Resources

About