Biochemical studies on pollen. Part XXXI. Effect of dibutyryl cyclic AMP and dibutyryl cyclic GMP on the changes in invertase, lipase, and some dehydrogenase activities during germination of pine pollen.

Abstract

Effects of exogenousBt2cAMP and Bt2cGMP on the changes in lipase, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase, and invertase activities were investigated during the germination of pollen from Pinus densiflora Sieb. et Zucc. Both nucleotides cooperatively restored the lipase activity which was depressed by exogenous sucrose, but had no effect on the change in glucose-6-phosphate dehydrogenase activity. Either Bt2cAMPor Bt2cGMPpartially restored the glutamate dehydrogenase activity which was depressed by exogenous sucrose. Bt2cGMPlowered the invertase activity during germination, whereas Bt2cAMP reversed this depression. Using inhibitors of RNAand protein synthesis, we suggested that Bt2cAMPand Bt2cGMPcontrol the invertase activity at the transcriptional and translational levels, respectively.