Biochemical studies on the production of Sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate

Atta, Houssam M.; Radwan, Hassan G.

doi:10.1016/j.jscs.2010.10.019

Cited by 5 publications

(6 citation statements)

References 41 publications

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“…This revealed the presence of a typical amine group, methyl groups of protein, C-H stretching vibrations of lipids, C-N group, OH group of ribose rings and C-OH groups of carbohydrate. Similar results have been reported by earlier workers [28,40,42].…”

Section: Discussionsupporting

confidence: 93%

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

2015

J Coast Life Med

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Section: Discussionsupporting

confidence: 93%

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

2015

J Coast Life Med

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“…DO5 was indole-negative but catalase-positive. Pseudomonas aeruginosa, AZ-SH-B8, was identified using API 20 NE kit during the production of antibiotic sparsomycin and recorded similar results such as nitrate reduction activity, negative indole production, and urease and oxidase production [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

et al. 2022

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Microorganisms are one of the main sources of antimicrobial agents and over 50% of antibiotics currently used in hospitals are metabolites from microbes. This study aimed to isolate microorganisms from the Dompoase landfill site, Kwame Nkrumah University Physics Garden, Kosiko River, and Ada Foah seashore of Ghana and screen their metabolites for antimicrobial activity. Forty-eight (48) microorganisms were isolated and their metabolites were screened against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Vibrio cholerae, Salmonella typhi, Pseudomonas aeruginosa, Streptococcus pyogenes, Proteus mirabilis, and Candida albicans using the agar well diffusion method. Ten (10) of the isolates exhibited antimicrobial activity. Isolate DO5, identified as P. aeruginosa isolate, from the Dompoase landfill site was selected for fermentation because it exhibited the highest activity against all the test organisms. DO5 produced optimum antimicrobial activity when fermented for 11 days at 30°C. In the agar diffusion method, the extract of isolate DO5 recorded zones of inhibition ranging between 11.67 ± 0.23 and 21.50 ± 0.71 mm. The MIC and MBC recorded for the DO5 extract ranged from 3.13–25.0 mg/mL and from 6.25–50.0 mg/mL, respectively. Column chromatography analysis yielded eight (8) subfractions from the DO5 extract. IR analysis revealed the presence of functional groups such as alcohols, esters, and hydrocarbons in the fractions. GC-MS analysis identified nine compounds that have been reported to have antimicrobial agents. The DO5 metabolites stand the chance to be developed into potent antibiotics for infection treatment.

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“…Appearance of pink spots indicates the presence of carbohydrates in biosurfactants [46]. That method was applied directly on a solution of pure biosurfactants by Atta et al [65]. The authors confirmed the presence of carbohydrates in the biosurfactants after the apparition of a violet or purple colour between the two layers formed by the solution of biosurfactants and the reagents.…”

Section: Screening Of Sugarsmentioning

confidence: 99%

Methods of Purification and Characterization of Biosurfactants: An Overview

Mouafo

Pahane

Arsène

et al. 2023

JABB

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Biosurfactants are amphiphilic molecules produced by several microorganisms including bacteria, filamentous fungi and yeasts. They are classified according to their chemical composition (glycolipids, lipopeptides, glycoproteins, glycolipopeptides, phospholipids) and their molecular weight (low and high molecular weight). Biosurfactants are currently used in several industrial fields (food industry, pharmacy, medicine, detergents, agriculture, cosmetics, oil recovery and bioremediation) owning to some of their specific properties such as their low toxicity, biocompatibility, biodegradability, environmentally-friendly, stability under extreme conditions (temperature, pH and salinity), structural diversity, production using renewable low-cost substrates, agreement with green chemistry and sustainability. Despite the properties of biosurfactants, there are few studies leading to their structural characterization. The most reported structures are from biosurfactants produced by Bacillus spp. (surfactins, fengycins, lichenysines) and Pseudomonas spp. (rhamnolipids). The majority of researches performed on biosurfactants emphasized their functional groups characterization. These biosurfactants have yet to be fully characterized at structural level. Hence, in this review, we highlight the different purification and structural characterization techniques which can be combined to provide information on the structure of the biosurfactants independently of its complexity. This will lead to enhance their application in some highly pointed industrial fields such as medicine and pharmacy.

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Biochemical studies on the production of Sparsomycin antibiotic by Pseudomonas aeurginosa, AZ-SH-B8 using plastic wastes as fermented substrate

Cited by 5 publications

References 41 publications

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

Polyunsaturated fatty acid-producing marine thraustochytrids: A potential source for antimicrobials

Antimicrobial Potential of Extract from a Pseudomonas aeruginosa Isolate

Methods of Purification and Characterization of Biosurfactants: An Overview

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