2016
DOI: 10.18805/asd.v36i4.6471
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Biochemical study of root extract of Withania somnifera (L.)plant through HPLC analysis

Abstract: Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and … Show more

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Cited by 7 publications
(8 citation statements)
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“…According to Kirson et al (1971), withanolides are produced via the oxidation of steroids. These compounds are localized in different amounts in the plant parts (Sangwan et al, 2004), mainly in leaves varying from 0.001 to 0.5% of dry weight (Dhar et al, 2015), in comparison with 0.023% of dry weight in the root (Gaurav et al, 2016).…”
Section: Morphological Characteristics Distribution Chemical Composit...mentioning
confidence: 99%
“…According to Kirson et al (1971), withanolides are produced via the oxidation of steroids. These compounds are localized in different amounts in the plant parts (Sangwan et al, 2004), mainly in leaves varying from 0.001 to 0.5% of dry weight (Dhar et al, 2015), in comparison with 0.023% of dry weight in the root (Gaurav et al, 2016).…”
Section: Morphological Characteristics Distribution Chemical Composit...mentioning
confidence: 99%
“…For hardening prepare a pot containing garden soil, farmyard manure, and vermiculite free from any pest or infectious micro-organism, transfer the regenerated plant to the pot and cover it with plastic film to maintain humidity for the fresh plantlets. Around 10-12 days transfer the plants which are now soil established in the glass house and after that transfer to the larger area or pots filled with mixed soil and sand in equal proportion under greenhouse conditions (Gaurav, et al, 2015(Gaurav, et al, , 2016(Gaurav, et al, , 2018Gaurav and Kumar, 2019;Bhoora, et al, 2015;Pant, et al, 2020Pant, et al, , 2021Pant, et al, , 2022Sharma et al, 2022;Saini et al, 2021Saini et al, , 2022Verma et al, 2021;Rawat et al, 2021).…”
Section: Regeneration and Hardeningmentioning
confidence: 99%
“…Plant regeneration and its growth both are synchronized by plant hormones, most physiological processes depend on several phytohormones (Wang and Irving, 2001;Gaurav et al, 2015b;Gaurav et al, 2018b). Various types of attentiveness of cytokinin (BAP) and auxins, (IBA) are used in a wide range of concentrations and many are mixed for the rebirth of the shoot.…”
Section: Regeneration Of Shootingmentioning
confidence: 99%