Biochemical surface modifications to titanium implants using the tresyl chlorideactivated method

Abstract

Tresyl chloride (2,2,2-trifluoroethanesulfonyl chloride)-activated method is an easy and simple method for immobilizing biological molecules onto a titanium surface. Tresyl chloride was directly dropped onto a titanium surface without any solvent. Tresylated titanium disks were immersed in a protein or peptide solution to give protein-or peptide-immobilized titanium. Frontier molecular orbital calculations suggested that tresylation of the OH compound improved its reactivity towards the amine groups of protein compounds. Quartz-crystal microbalance-dissipation measurements suggested that ionic interaction between the oxygen of the terminal hydroxyl groups of titanium and the nitrogen of fibronectin was important for fibronectin immobilization. Initial cell attachment was enhanced by fibronectin or collagen immobilization. Fibronectin and fibronectin-derived peptide immobilization improved the expression of specific genes related to bone formation. The immobilization of cytokine onto titanium enhanced bone formation following its implantation into rat femur defects. The tresyl chloride-activated method is useful for immobilizing biological molecules onto titanium surfaces.

Role of rhBMP-7, Fibronectin, And Type I Collagen in Dental Implant Osseointegration Process: An Initial Pilot Study on Minipig Animals

“…This result confirms the reported ability of FN to facilitate early osteoblast adhesion and differentiation and agrees with previous observations in vitro on human and murine osteoblast-like cells, and in vivo after positioning of FN-modified dental titanium implants in dogs [ 66 , 69 , 70 ].…”

Cellular responses of histatin-derived peptides immobilized titanium surface using a tresyl chloride-activated method

“…Furthermore, ALP activity was crucial for the mineralization and also indicative for the maturation of the osteoblasts 50) . Hayakawa et al also reported change in ALP activity of MC3T3-E1 cells cultured on fibronectin-immobilized Ti surface after 3, 15, and 52 days 7) .…”

“…Histatin-1, JH8194, and mixed histatin-1 with JH8194 peptides at ratio 1:1 were reconstituted as the manufacturer's advice. Tresylated Ti disks were immersed in 10 µM peptides solution at 37°C for 1 day 7) . Ti disks were washed twice with phosphate buffered saline (PBS) and used for entire experiments.…”

“…Moreover, tresyl chloride-activated method is a wet process. Therefore, we can immobilized the proteins or cytokines 4) on the any shape of titanium such as Ti disks 7) , dental implant 8) , Ti web, and Ti wire 9) .…”

Influence of Fibronectin Immobilization on the Orientation of Collagen Bundles in Soft Tissue around Zirconia Implants

“…Aung et al grafted an allylamine and fibronectin composite surface onto zirconia and reported that the fibronectin grafted surface significantly stimulated the bioactivity of osteoblast-like cells 11) . Hayakawa et al developed a new method for immobilizing proteins or cytokines onto titanium, called the tresyl chloride-activated method 12) . Better bone response has been reported by immobilizing TGF-β2 or fibronectin onto titanium by the tresyl chloride-activated method 13,14) .…”