Biochemical toxicity and transcriptome aberration induced by dinotefuran in Bombyx mori

Xu, Shiliang; Hao, Zhihua; Li, Yinghui; Zhou, Yanyan; Shao, Ruixi; Chen, Rui; Zheng, Meidan; Xu, Ye; Wang, Huabing

doi:10.1016/j.envpol.2022.119562

Cited by 16 publications

(7 citation statements)

References 56 publications

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“…21 In silkworm larvae, after stimulation with Dinotefuran, a neonicotinic insecticide targeting on nAChR, expression levels of the genes in the PI3K/AKT and ERK/CREB pathways are significantly improved. 35 Besides, we found that PI3K can regulate feeding of O. furnacalis larvae through affecting expression levels and activities of ⊍ Amylase and lipase. 20 Phytohemagglutinin (PHA-M) is a plant lectin, extracted from kidney beans.…”

Section: Discussionmentioning

confidence: 82%

“…The loss of CREB function in neurons leads to the reduction of glycogen and lipid storage as well as increase of sensitivity to starvation 21 . In silkworm larvae, after stimulation with Dinotefuran, a neonicotinic insecticide targeting on nAChR, expression levels of the genes in the PI3K/AKT and ERK/CREB pathways are significantly improved 35 . Besides, we found that PI3K can regulate feeding of O. furnacalis larvae through affecting expression levels and activities of α Amylase and lipase 20 …”

Section: Discussionmentioning

confidence: 89%

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The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

Jiang

Shi

Yang

et al. 2023

Pest Management Science

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BackgroundFeeding is the basis of animal survival and reproduction. In insects, the neuropeptide F (NPF), a homologous polypeptide of NPY in vertebrates, plays an important role in regulation of feeding behavior. However, relatively little has been known about the molecular mechanism of feeding.ResultsIn this study, we show that the cholinergic pathway is very important in signaling transmission of NPF feeding regulation in Ostrinia furnacalis larvae, in which the choline acetyltransferase (ChAT), the vesicular acetylcholine transporter (vAChT) in presynaptic membrane and the nicotinic acetylcholine receptor (nAChR) in postsynaptic membrane are positively regulated by NPF, while the ace1 and ace2 encoding the acetylcholinesterase (AChE) are negatively regulated by NPF, leading to a balance of acetylcholine (ACh)‐the excitatory transmitter. More, the cholinergic pathway further transmits signaling to the downstream pathways of the phosphoInositide‐3 kinase (PI3K) and the cAMP responsive element binding protein (CREB), respectively.ConclusionThe cholinergic transmission, positively regulated by NPF, is involved in feeding of O. furnacalis larvae via downstream PI3K and the CREB pathways, respectively. The deexcitation of cell cholinergic pathway or inhibition of PI3K and CREB lead to decreases of larval feeding amount. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 89%

The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

Jiang

Shi

Yang

et al. 2023

Pest Management Science

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“…Consequently, there is a pressing need to investigate the toxicological impacts of heavy metals and elucidate detoxification mechanisms in silkworms, given the substantial environmental accumulation of heavy metals (16). Owing to its remarkable characteristics and longstanding history of artificial domestication, the silkworm has obtained considerable attention from scientists across various fields, particularly in recent years, encompassing environmental pollution, nanomaterial toxicity assessment, pesticides, and medicine (17)(18)(19)(20). The Chinese Oak silkworm (Antheraea pernyi) among the best-known species of wild silkworms has been used as a well-known wild silk moth in sericultural and medicine industry for hundreds of years.…”

Section: Open Access Edited Bymentioning

confidence: 99%

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Wen,

Wang,

et al. 2024

Front. Vet. Sci.

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Mercury (Hg) contamination poses a global threat to the environment, given its elevated ecotoxicity. Herein, we employed the lepidopteran model insect, silkworm (Bombyx mori), to systematically investigate the toxic effects of Hg-stress across its growth and development, histomorphology, antioxidant enzyme activities, and transcriptome responses. High doses of Hg exposure induced evident poisoning symptoms, markedly impeding the growth of silkworm larvae and escalating mortality in a dose-dependent manner. Under Hg exposure, the histomorphology of both the midgut and fat body exhibited impairments. Carboxylesterase (CarE) activity was increased in both midgut and fat body tissues responding to Hg treatment. Conversely, glutathione S-transferase (GST) levels increased in the fat body but decreased in the midgut. The transcriptomic analysis revealed that the response induced by Hg stress involved multiple metabolism processes. Significantly differently expressed genes (DEGs) exhibited strong associations with oxidative phosphorylation, nutrient metabolisms, insect hormone biosynthesis, lysosome, ribosome biogenesis in eukaryotes, and ribosome pathways in the midgut or the fat body. The findings implied that exposure to Hg might induce the oxidative stress response, attempting to compensate for impaired metabolism. Concurrently, disruptions in nutrient metabolism and insect hormone activity might hinder growth and development, leading to immune dysfunction in silkworms. These insights significantly advance our theoretical understanding of the potential mechanisms underlying Hg toxicity in invertebrate organisms.

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“…RNA-seq analysis unveils a genome-wide gene expression profile that provides a relatively comprehensive view of an organism's biological responses to environmental stresses. 39 To gain insights into the genetic regulation and to better understand the effect of AuCu−Cu 2 S on silkworms, RNA-seq-based transcriptional analysis of the midgut and fat body was performed. Twelve RNA-sequencing libraries were generated from 4 samples (3200 ppm-ZC (midgut), 3200 ppm-ZFT (fat body), and 0 ppm-ZC, 0 ppm-ZFT) with 3 replicates each.…”

Section: Analysis Of Transcriptomementioning

confidence: 99%

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

Andoh,

Shi,

et al. 2023

ACS Biomater. Sci. Eng.

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AuCu–Cu2S nanocomposites are unique materials with exceptional properties that have recently received a lot of interest. However, little is known about their potential toxicity in terrestrial organisms and their subsequent effects on the environment. Therefore, it is essential to develop effective methodologies for evaluating AuCu–Cu2S nanocomposites in biological systems. This study reports the biological evaluation of the AuCu–Cu2S nanocomposite from animal and cell entity levels. The Bombyx mori silkworm was used as a model organism to study the effects of different concentrations of AuCu–Cu2S on silkworm development. Transcriptome analysis was also carried out to examine the genetic modulation exerted by the treatment. Moreover, biocompatibility and cytotoxicity of AuCu–Cu2S were evaluated in human bronchial epithelial cells 16HBE, human lung adenocarcinoma, and the insect Spodoptera frugiperda cell sf9 cell lines. The results showed that although AuCu–Cu2S at ≤400 ppm can prolong the eating habit of silkworms and promote the weight of the cocoon layer, there was an increase in silkworm mortality and a decrease in moth formation at a concentration of ≥800 ppm. The genetic regulation by AuCu–Cu2S treatment showed varying effects in the silkworm, primarily related to functions such as transport and catabolism, metabolism of cofactors and vitamins, xenobiotic biodegradation, amino acid, and carbohydrate. 16HBE, PC-9, and sf9 treated with 300 ppm of AuCu–Cu2S showed viability percentages of 60, 20, and 90%, respectively. Thus, AuCu–Cu2S at low concentrations serves as a safe and biocompatible material for the sf9 cell lines but is lethal to 16HBE and PC-9. This research could aid in understanding the biological effects and biocompatibility of AuCu–Cu2S nanocomposites, particularly in the field of biochemistry; however, the mechanisms involved need further exploration.

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Biochemical toxicity and transcriptome aberration induced by dinotefuran in Bombyx mori

Cited by 16 publications

References 56 publications

The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

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Biochemical toxicity and transcriptome aberration induced by dinotefuran in Bombyx mori

Cited by 16 publications

References 56 publications

The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

The cholinergic pathway transmits signals of neuropeptide F to regulate feeding of Ostrinia furnacalis larvae

Effect of acute exposure of Hg on physiological parameters and transcriptome expression in silkworms (Bombyx mori)

Cytotoxicity of AuCu–Cu2S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines

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Cytotoxicity of AuCu–Cu₂S Nanocomposites: Implications for Biological Evaluation of the Nanocomposite Effect on Bombyx mori Silkworms and Cell Lines