Biocidal effect to fish pathogens of Aqua farmsafe^®composed of yucca extract and didecyldimethylammonium chloride

Abstract

In this study, the disinfectant efficacy of Aqua farmsafe ® , composed of didecyldimethylammonium chloride (DDAC) and yucca extract was evaluated against Salmonella typhimurium and fish pathogens. Determination of the anti-microbial or anti-viral efficacy of the disinfectant was based on Animal, Plant and Fisheries Quarantine and Inspection Agency Regulation No. 2011-26, Korea. Anti-bacterial efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4℃. Aqua farmsafe and test bacteria or virus were diluted with distilled water (DW), standard hard water (SW) or organic matter dilution (OM) according to treatment condition. Under the our results, disinfectant efficacy of Aqua farmsafe ® possesses 30~40 fold against fish pathogens including bacteria and virus compared to that on animal pathogenic bacteria, S. typhimurim. As the efficacy of Aqua farmsafe ® against fish pathogen was investigated in vitro, a controlled field trial is required to determine whether the use of Aqua framsafe ® will be able to reduce fish diseases.