Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

Zhang, Min; Xue, Yanan; Liu, Min; Zhuo, Ren‐Xi; Huang, Shi‐Wen

doi:10.1007/s11671-010-9716-9

Cited by 9 publications

(5 citation statements)

References 49 publications

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“…Using 750 kDa PEI as a stabilizer, particle assembly was templated by the aggregated polymer [27,29,33]. The results are comparable to previous reports with various PEI systems at constant charge ratios [34,35]. Interestingly, with the 10 kDa PEI stabilizer, doubling the total solids concentration resulted in a two-fold increase in particle size from 143 ± 8 nm to 319 ± 185 nm while maintaining a PDI less than 0.300 (Table S7).…”

Section: Resultssupporting

confidence: 74%

Rapid, Single-Step Protein Encapsulation via Flash NanoPrecipitation

2019

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Flash NanoPrecipitation (FNP) is a rapid method for encapsulating hydrophobic materials in polymer nanoparticles with high loading capacity. Encapsulating biologics such as proteins remains a challenge due to their low hydrophobicity (logP < 6) and current methods require multiple processing steps. In this work, we report rapid, single-step protein encapsulation via FNP using bovine serum albumin (BSA) as a model protein. Nanoparticle formation involves complexation and precipitation of protein with tannic acid and stabilization with a cationic polyelectrolyte. Nanoparticle self-assembly is driven by hydrogen bonding and electrostatic interactions. Using this approach, high encapsulation efficiency (up to ~80%) of protein can be achieved. The resulting nanoparticles are stable at physiological pH and ionic strength. Overall, FNP is a rapid, efficient platform for encapsulating proteins for various applications.

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Section: Resultssupporting

confidence: 74%

Rapid, Single-Step Protein Encapsulation via Flash NanoPrecipitation

2019

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“…Due to their high cationic charge density at physiological pH, PEIs are able to form non-covalent complexes with DNA, siRNA, and antisense oligodeoxynucleotide. Therefore, PEIs hold a prominent position among the polycationic polymers used for gene delivery [ 16 - 18 ]. The intracellular release of PEI/nucleic acids complexes from endosomes is considered as relying on the protonation of amines in the PEI molecule, which leading to osmotic swelling and subsequent burst of the endosomes.…”

Section: Introductionmentioning

confidence: 99%

PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells

et al. 2011

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The biggest challenge in the field of gene therapy is how to effectively deliver target genes to special cells. This study aimed to develop a new type of poly(D,L-lactide-co-glycolide) (PLGA)-based nanoparticles for gene delivery, which are capable of overcoming the disadvantages of polyethylenimine (PEI)- or cationic liposome-based gene carrier, such as the cytotoxicity induced by excess positive charge, as well as the aggregation on the cell surface. The PLGA-based nanoparticles presented in this study were synthesized by emulsion evaporation method and characterized by transmission electron microscopy, dynamic light scattering, and energy dispersive spectroscopy. The size of PLGA/PEI nanoparticles in phosphate-buffered saline (PBS) was about 60 nm at the optimal charge ratio. Without observable aggregation, the nanoparticles showed a better monodispersity. The PLGA-based nanoparticles were used as vector carrier for miRNA transfection in HepG2 cells. It exhibited a higher transfection efficiency and lower cytotoxicity in HepG2 cells compared to the PEI/DNA complex. The N/P ratio (ratio of the polymer nitrogen to the DNA phosphate) 6 of the PLGA/PEI/DNA nanocomplex displays the best property among various N/P proportions, yielding similar transfection efficiency when compared to Lipofectamine/DNA lipoplexes. Moreover, nanocomplex shows better serum compatibility than commercial liposome. PLGA nanocomplexes obviously accumulate in tumor cells after transfection, which indicate that the complexes contribute to cellular uptake of pDNA and pronouncedly enhance the treatment effect of miR-26a by inducing cell cycle arrest. Therefore, these results demonstrate that PLGA/PEI nanoparticles are promising non-viral vectors for gene delivery.

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“…The average diameters of PIC flower micelles composed of AEA/EM 27 , AEA/EM 53 and AEA/EM 106 estimated from TEM were 14 ± 4, 53 ± 2 and 49 ± 5 nm, respectively, which were smaller than the 2R h values, estimated from DLS (Table 2). This implied that the PIC micelles shrank after the removal of water in dry state to measure TEM [29]. Spherical shape for PIC micelles can be observed, suggesting that the aggregates are individual flower micelles without intermicellar aggregation.…”

Section: The Density Of Pic Micelles (D Pic ) Can Be Calculated Bymentioning

confidence: 99%

Polyion Complex (PIC) Flower-shaped Nano-micelles formed from Anionic Triblock and Cationic Diblock Copolymers

Yusa¹

2014

NTMB

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Poly (sodium 2-(acrylamido)-2-methylpropanesulfonate)-block-poly(ethylene glycol)-block-poly(sodium 2-(acrylamido)-2-methylpropanesulfonate) (PAMPS48-PEG227-PAMPS48) and poly(ethylene glycol)-block-poly(3-(methacryloylamino)propyl) trimethylammonium chloride (PEG47-PMAPTACm, m = 27,53, and 106) with different PMAPTAC chain lengths were prepared via reversible addition-fragmentation chain transfer controlled living radical polymerization using PEG-based macro chain transfer agents. The subscript numbers in abbreviation represent the degree of polymerization of the block. Mixing of aqueous solutions of the oppositely charged anionic triblock copolymer, PAMPS48-PEG227-PAMPS48 and cationic diblock copolymers, PEG47-PMAPTACm led to the spontaneous formation of Polyion Complex (PIC) micelles. The characterization data of NMR, light scattering, ζ-potential, and transmission electron microscopic measurements indicated that the PIC flower micelles were formed comprising the segregated PIC core and surrounded hydrophilic linear and loop PEG shell chains. Aggregation number and hydrodynamic radius achieved maximum values when anionic and cationic block chain lengths of the block copolymers were approximately the same.

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Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

Cited by 9 publications

References 49 publications

Rapid, Single-Step Protein Encapsulation via Flash NanoPrecipitation

Rapid, Single-Step Protein Encapsulation via Flash NanoPrecipitation

PLGA-based gene delivering nanoparticle enhance suppression effect of miRNA in HePG2 cells

Polyion Complex (PIC) Flower-shaped Nano-micelles formed from Anionic Triblock and Cationic Diblock Copolymers

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