Biocompatibility and Antibacterial Effects of 6-Deoxy-6-Aminoethyleneamino Cellulose

Abstract

Recently, there is a need of alternatives to antibiotics due to increasing antibiotic-resistant microorganism. Promising classes of bioactive polymers are 6-deoxy-6-amino cellulose derivatives. The purpose of the study was the assessment of the biocompatibility of 6-deoxy-6-aminoethyleneamino cellulose (AEAC) with different degree of substitution (DS). HaCaT keratinocyte cell viability was analyzed by measuring the cellular ATP content. The antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae was examined by microplate laser nephelometry. Thus, the ratio of half-maximal lethal concentration (LC 50 ) and half-maximal inhibitory concentration (IC 50 ) was calculated and described as biocompatibility index. The study revealed that biocompatibility of AEAC depends on the DS. AEAC of low DS (0.3) showed the best biocompatibility.

Synthesis, Characterization and Cytotoxicity Studies of Aminated Microcrystalline Cellulose Derivatives against Melanoma and Breast Cancer Cell Lines

“…Literature survey showed Finger S et al reported 6-deoxy-6aminoethyleneamino cellulose with different degree of substitution (DS) via tosylation against keratinocyte cell (HaCaT). Their studies concluded that lowest DS (0.3) was most biocompatible with (HaCaT) [ 60 ]. While in another study, El-Sayed et al carried out cytotoxic studies against BJ1 of 3-aminopropyltrimethoxysilane and TiO 2 grafted tosylated cellulose.…”