Biocompatible formulations based on mycosynthesized iron oxide nanoparticles: Fabrication, characterization, and biological investigation

Abstract

The current study was carried out to synthesize iron oxide nanoparticles (IONPs) via green reduction method from a wild mushroom collected from Quaid‐i‐Azam University, Islamabad, Pakistan. The collected fungus was identified as Daedalea sp. based on morphological characteristics. Prepared NPs were produced from iron chloride hexahydrate with fungal filtrate via combustion method. The as prepared NPs were characterized by using different techniques for example, scanning electron microscopy (SEM), X‐ray diffractions (XRD), Fourier transform infrared spectroscopy (FT‐IR), and ultraviolet visible (UV‐Vis) spectroscopy. Morphology and size of the NPs were determined by SEM analysis. XRD study revealed crystalline nature of IONPs. The FT‐IR spectrum exhibited peak at 3390.26 cm−1 stretching that described the strong O–H band of the alcohol associated with mushroom texture. The major IONPs dose (0.75 mg/ml) demonstrated 71% growth inhibition against Aspergillus. Excellent antibacterial activities against Pseudomonas aeruginosa (28 mm), and Klesbsilla pneumonia (28 mm) were represented by the fabricated NPs. Further, highest reducing power (53.22 ± 0.72 µg AAE/mg) was shown by the highest administrated dose (400 µg/ml). Maximum 2,2‐diphenyl‐1‐picrylhydrazyl and trolox antioxidant activity free radical scavenging activities at 400 µg/ml IONPs concentration were noted as 51.29 ± 0.48, and 83.12 ± 0.28 trolox equivalent antioxidant capacity, respectively. In brief, the negligible hemolytic activity against human red blood cells at the highest concentration (400 µg/ml), as well as, moderate antioxidant activities at low concentration suggest the application of the fabricated NPs in environmentally sound viable hygiene production.

Wild Mushroom (Daedalea sp) as Biosource for Silver Nanoparticles: From Synthesis to Enhance antimicrobial, Biomedical & Environmental Applications

“…2a and 2b. Additionally, TEM analysis con rmed morphology and dimension (Kamal, Saba, Kamal, et al, 2023). The mean size of NPs as calculated from TEM micrographs via ImageJ software was found to be 21.4 nm.…”

“…Then after, Centrifugation of incubated sample was carried out at 10000rpm for 10minutes. A supernatant was created, and 100 l of it was poured into 96-well plates (Kamal, Saba, & Farooq, 2023). The absorbance of the obtained supernatant was measured at 541nm using a spectrophotometer.…”

Hierarchical plant extracts in silver nanoparticles preparation: Minuscular survey to achieve enhanced bioactivities

“…To coup with this issue, various stabilizing methods, such as, dendrimers, polymeric gels, and some natural entities are incorporated to protect NPs active surface for enhanced efficacy. Natural entities used for NPs reduction purpose are various plants, fungi and bacteria to carry out biosynthesis of NPs under green nano-technological protocols [ 11 , 12 ].…”

Synthesis and Characterization of Zinc Oxide Nanoparticles at Different pH Values from Clinopodium vulgare L. and Their Assessment as an Antimicrobial Agent and Biomedical Application

“…Metal and metal oxide NPs are found to be antioxidants and antibacterial in practical applications [ 6 ]. Low concentrations of metal oxide NPs, including zinc (Zn), magnesium (Mg), and titanium (Ti) oxides, inhibit microbial growth significantly [ 7 ]. Moreover, the metal NPs can be generated using a green strategy that employs various plant components as reducing agents.…”