Bioconjugated quantum dots for in vivo molecular and cellular imaging☆

Smith, Andrew; Duan, Hongwei; Mohs, Aaron M.; Nie, Shuming

doi:10.1016/j.addr.2008.03.015

Cited by 1,086 publications

(784 citation statements)

References 173 publications

(282 reference statements)

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“…In the field of nanomedical research, quantum dots (QDots) take an important place as powerful probes for fluorescence imaging [1][2][3]. QDots are colloidal nanocrystals with a core diameter that ranges between 1 and 10 nm and that are built up of semiconductor materials, typically combinations of groups 12 and 16 (e.g.…”

Section: Introductionmentioning

confidence: 99%

The performance of gradient alloy quantum dots in cell labeling

Soenen¹,

Manshian²,

Himmelreich³

et al. 2014

Biomaterials

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The interest in using quantum dots (QDots) as highly fluorescent and photostable nanoparticles in biomedicine is vastly increasing. One major hurdle that slows down the (pre)clinical translation of QDots is their potential toxicity. Several strategies have been employed to optimize common coreshell QDots, such as the use of gradient alloy (GA)-QDots. These particles no longer have a sizedependent emission wavelength, but the emission rather depends on the chemical composition of the gradient layer. Therefore, particles of identical sizes but with emission maxima spanning the entire visible spectrum can be generated. In the present study, two types of GA-QDots are studied with respect to their cytotoxicity and cellular uptake. A multiparametric cytotoxicity approach reveals concentration-dependent effects on cell viability, oxidative stress, cell morphology and cell functionality (stem cell differentiation and neurite outgrowth), where the particles are very robust against environmentally-induced breakdown. Non-toxic concentrations are defined and compared to common core-shell QDots analyzed under identical conditions. Additionally, this value is translated into a functional value by analyzing the potential of the particles for cell visualization. Interestingly, these particles result in clear endosomal localization, where different particles result in identical intracellular distributions. This is in contrast with CdTe QDots with the same surface coating, which resulted in clearly distinct intracellular distributions as a result of differences in nanoparticle diameter.The GA-QDots are therefore ideal platforms for cell labeling studies given their high brightness, low cytotoxicity and identical sizes, resulting in highly similar intracellular particle distributions which offer a lot of potential for optimizing drug delivery strategies.

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Section: Introductionmentioning

confidence: 99%

The performance of gradient alloy quantum dots in cell labeling

Soenen¹,

Manshian²,

Himmelreich³

et al. 2014

Biomaterials

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show abstract

“…Some reports have mentioned that the instability of the surface coating might caused the QDs core to degrade or allow air oxidation and result in the release of cadmium ions to the biological enviroment [113]. Some reports have demonstrated that the ZnS capping might protect the core from air oxidation [10]. However, the ZnS shell can be damaged under UV light source.…”

Section: Toxicity Of Quantum Dots In Vitro and In Vivomentioning

confidence: 99%

“…Quantum dots (QDs), semiconductor nanocrystals small enough to exhibit size-dependent properties, have generated tremendous interest due to their unique optical properties [1][2][3][4][5], including broad excitation spectra [6], narrow, tunable [7] and symmetric emission spectra, and excellent photostability [8][9][10][11][12][13][14]. Also, QDs are highly efficient multi-photon absorbers that can be potentially useful for three dimensional multi-photon microscopy and imaging [15], which is a rapidly developing area for both biological and medical applications [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Aqueous phase synthesis of CdTe quantum dots for biophotonics

Yong

Law

Roy

et al. 2010

Journal of Biophotonics

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Over the past few years, CdTe quantum dots have been demonstrated as powerful probes for biophotonics applications. The aqueous phase synthesis technique remains the best approach to make high quality CdTe QDs in a single‐pot process. CdTe QDs prepared directly in the aqueous phase can have quantum yield as high as 80%. In addition, the surface of CdTe QDs prepared using the aqueous phase technique is functionalized with reactive groups that enable them to be directly conjugated with specific ligands for targeted delivery and sensing. In this contribution, we review recent progress in fabricating aqueous CdTe QDs and exploiting their optical properties in novel approaches to biomedical imaging and sensing applications. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…These optical properties are not owned by all the current fluorescent probes. Currently, QDs have been applied in the imaging of biological macromolecules and cells both in vitro and in vivo (5)(6)(7). Non-invasive in vivo imaging has been used for the investigation of tumor development (8)(9)(10), early diagnosis of cancer (10), the transportation of drugs in vivo (11), and monitoring of therapeutic responses in vivo (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

et al. 2012

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Abstract. In this study, we applied near-infrared fluorescent quantum dots (NIRF-QDs) for non-invasive in vivo and in situ imaging of head and neck squamous cell carcinoma (HNSCC). The U14 squamous cancer cell line with high expression of epidermal growth factor receptor (EGFR) was implanted subcutaneously into the head and neck regions of nude mice to establish HNSCC models. NIRF-QDs with an emission wavelength of 800 nm (NIRF-QD800) were conjugated with EGFR monoclonal antibodies to develop the QD800-EGFR Ab probe. In vivo and in vitro studies demonstrated that the QD800-EGFR Ab probe can specifically bind EGFR expressed on U14 cells. U14 squamous cell carcinoma in the head and neck can be clearly visualized by in vivo imaging after intravenous injection of QD800-EGFR Ab probes. The results suggested that in situ imaging using NIRF-QD-EGFR Ab probes has unique advantages and prospects for the investigation of tumor development, early diagnosis and personalized therapy of HNSCC. IntroductionNon-invasive in vivo and in situ imaging of tumor cells plays an important role in studying the occurrence and development of cancers, making early diagnosis and conducting personalized therapies. This has been a difficult area due to the lack of highly sensitive imaging substances and specific marker for each particular type of tumor. Quantum dots (QDs) were developed recently and have shown great prospect in the noninvasive imaging of tumors (1,2).QD is a type of nanocrystal composed of elements belonging to Ⅱ-Ⅵ or Ⅲ-Ⅴ groups with a diameter of 2-10 nm. In comparison to the conventional organic fluorescent dyes and fluorescent proteins, QDs have unique optical properties, e.g., broad and continuous distribution of the excitation spectrum, narrow and symmetric emission spectrum, strong fluorescence and high photochemical stability. In addition, QDs are less prone to photobleaching and the spectrum of any points from ultraviolet to the near infrared can be obtained by changing the size and composition of QDs (3,4). These optical properties are not owned by all the current fluorescent probes. Currently, QDs have been applied in the imaging of biological macromolecules and cells both in vitro and in vivo (5-7). Non-invasive in vivo imaging has been used for the investigation of tumor development (8-10), early diagnosis of cancer (10), the transportation of drugs in vivo (11), and monitoring of therapeutic responses in vivo (11)(12)(13). These studies have demonstrated the unique advantages of QDs in the imaging of cells both in vitro and in vivo. Particularly, the recently developed near-infrared fluorescent quantum dot (NIRF-QD) with an emission range of 700-900 nm has the advantage of avoiding the interference of tissue auto fluorescence (400-600 nm). NIRF-QDs have strong tissue penetration, but do not have radiation and are not harmful in vivo. Therefore, NIRF-QDs are extremely suitable for non-invasive in vivo imaging (14,15).NIRF-QDs were conjugated with prostate specific membrane antigen monoclonal antibody (PSMA), Her2 ...

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Bioconjugated quantum dots for in vivo molecular and cellular imaging☆

Cited by 1,086 publications

References 173 publications

The performance of gradient alloy quantum dots in cell labeling

The performance of gradient alloy quantum dots in cell labeling

Aqueous phase synthesis of CdTe quantum dots for biophotonics

In vivo and in situ imaging of head and neck squamous cell carcinoma using near-infrared fluorescent quantum dot probes conjugated with epidermal growth factor receptor monoclonal antibodies in mice

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