Bioconjugation of Meldrum's acid activated furan: A detergent compatible assay for protein quantitation

Ajayan, Kalyani; Sainath, S.B.; Sadik, Ajmal; Nair, Manu Mohanan; Nair, Anju M.; S, Karthika K.; Vijayakumar, Anagha; Nair, Sudarslal Sadasivan; Nair, Bipin G.; R, Prakash Chandran; Nair, Sobha Vijayan

doi:10.1016/j.ab.2022.114998

Cited by 6 publications

(6 citation statements)

References 30 publications

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“…MAFC can therefore not only be used as a detecting agent for lysine and arginine at neutral pH but also as a selective detection agent for P in biological samples at pH 11. Recently, Ajayan et al demonstrated the possibility of activated furan to quantify protein concentration via the colorimetric sensing with MAFC (see Figure 4 C) [ 17 ]. MAFC was hereby dissolved in DMSO and either used directly for the sensing of hydrophobic proteins or in combination with proteins dissolved in aqueous solutions.…”

Section: Meldrum’s Acid-activated Furan Conjugate As Amine Detecting ...mentioning

confidence: 99%

“…( C ) Overview of findings of protein quantification studies with MAFC as detection agent. Reprinted and adapted with permission from [ 17 ]. ( D ) Schematic depiction of high-throughput screening system for optimizations of enzymatic polyamide and polyurethane degradation studies.…”

Section: Figurementioning

confidence: 99%

“…Various techniques have been developed based on different detection agents and sensing methods. But often, these techniques involve not only expensive equipment but also difficult on-site analysis due to poor portability, tedious analysis procedures, or low selectivity and specificity [ 17 , 18 ]. Thus, new inexpensive methods providing facile operation, rapid analyte quantification, and high sensing capacity are always searched for.…”

Section: Introductionmentioning

confidence: 99%

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Meldrum’s Acid Furfural Conjugate MAFC: A New Entry as Chromogenic Sensor for Specific Amine Identification

Zeußel,

Singh

2023

Molecules

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Bioactive amines are highly relevant for clinical and industrial application to ensure the metabolic status of a biological process. Apart from this, generally, amine identification is a key step in various bioorganic processes ranging from protein chemistry to biomaterial fabrication. However, many amines have a negative impact on the environment and the excess intake of amines can have tremendous adverse health effects. Thus, easy, fast, sensitive, and reliable sensing methods for amine identification are strongly searched for. In the past few years, Meldrum’s acid furfural conjugate (MAFC) has been extensively explored as a starting material for the synthesis of photoswitchable donor–acceptor Stenhouse adducts (DASA). DASA formation hereby results from the rapid reaction of MAFC with primary and secondary amines, which has so far been demonstrated through numerous publications for different applications. The linear form of the MAFC-based DASA exhibits intense pink coloration due to its linear conjugated triene-2-ol conformation, which has inspired researchers to use this easy synthesizable molecule as an optical sensor for primary, secondary, and biogenic amines. Due to its new entry into amine identification, a collection of the literature exclusively on MAFC is demanded. In this mini review, we intend to present the state-of-the-art of MAFC as an optical molecular sensor in hopes to motivate researchers to find even more applications of MAFC-based sensors and methods that pave the way to their usage in medicinal applications.

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Section: Meldrum’s Acid-activated Furan Conjugate As Amine Detecting ...mentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Meldrum’s Acid Furfural Conjugate MAFC: A New Entry as Chromogenic Sensor for Specific Amine Identification

Zeußel,

Singh

2023

Molecules

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“…This study is focused on the efficacy of activated furan compounds, Meldrum’s acid activated furan (MAF) and 1,3-dimethyl barbituric acid activated furan (BAF) as anti-virulent agents. Recently we reported MAF as an effective agent for protein quantification ( Ajayan et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Mitigation of quorum sensing mediated virulence factors of Pseudomonas aeruginosa: the role of Meldrum’s acid activated furan

Sadik,

Viswaswar,

Rajamoney

et al. 2024

Front. Microbiol.

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The rapid emergence of drug resistant pathogens is a major threat which has warranted the development of alternative strategies to combat infectious diseases. In this work, we have tested the anti-virulent activity of Meldrum’s acid activated furan (MAF) and 1,3-dimethyl barbituric acid activated furan (BAF) against Chromobacterium violaceum and Pseudomonas aeruginosa. It was found that MAF significantly reduced the violacein production and biofilm formation of C. violaceum at sub-inhibitory concentrations. The quorum sensing (QS) regulated virulence factors of P. aeruginosa including biofilm formation, motility, pigment production, and elastase activity were also found to be reduced considerably at sub-inhibitory concentrations of MAF. Additionally, MAF downregulated the expression of genes in the QS circuitry of P. aeruginosa, demonstrating the potential of MAF in lowering the pathogenicity of P. aeruginosa. In silico studies demonstrated the potential of MAF to compete with the signaling molecules of C. violaceum and P. aeruginosa for the QS receptor interaction. In vivo studies using Caenorhabditis elegans demonstrated the anti-pathogenicity of MAF by enhancing the survival of P. aeruginosa-infected C. elegans. These results suggest that activated furan compounds could be potential inhibitors of QS-mediated virulence factors in C. violaceum and P. aeruginosa, encouraging their use in combating multidrug-resistant pathogens.

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“…Protein quantitation, the process of determining the exact concentration of a targeted protein in a sample, is an essential element of biological and chemical research, biotechnology development, drug discovery, and clinical diagnostic [1][2][3][4]. Specifically, accurate quantification of protein concentrations is required for determination of protein expression levels, monitoring protein-protein interactions, evaluating drug efficacy, identifying disease biomarkers, evaluating virus-host interactions, drug screening and development [1,2,4,5].…”

Section: Introductionmentioning

confidence: 99%

DNA-based assay for calorimetric determination of protein concentrations in pure or mixed solutions

Eskew,

Reardon,

Benight

2024

PLoS ONE

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It was recently reported that values of the transition heat capacities, as measured by differential scanning calorimetry, for two globular proteins and a short DNA hairpin in NaCl buffer are essentially equivalent, at equal concentrations (mg/mL). To validate the broad applicability of this phenomenon, additional evidence for this equivalence is presented that reveals it does not depend on DNA sequence, buffer salt, or transition temperature (Tm). Based on the equivalence of transition heat capacities, a calorimetric method was devised to determine protein concentrations in pure and complex solutions. The scheme uses direct comparisons between the thermodynamic stability of a short DNA hairpin standard of known concentration, and thermodynamic stability of protein solutions of unknown concentrations. Sequences of two DNA hairpins were designed to confer a near 20°C difference in their Tm values. In all cases, evaluated protein concentrations determined from the DNA standard curves agreed with the UV-Vis concentration for monomeric proteins. For multimeric proteins evaluated concentrations were greater than determined by UV-Vis suggesting the calorimetric approach can also be an indicator of molecular stoichiometry.

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Bioconjugation of Meldrum's acid activated furan: A detergent compatible assay for protein quantitation

Cited by 6 publications

References 30 publications

Meldrum’s Acid Furfural Conjugate MAFC: A New Entry as Chromogenic Sensor for Specific Amine Identification

Meldrum’s Acid Furfural Conjugate MAFC: A New Entry as Chromogenic Sensor for Specific Amine Identification

Mitigation of quorum sensing mediated virulence factors of Pseudomonas aeruginosa: the role of Meldrum’s acid activated furan

DNA-based assay for calorimetric determination of protein concentrations in pure or mixed solutions

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