Biodegradation of Green HE4B: Co-substrate effect, biotransformation enzymes and metabolite toxicity analysis

Kalme, Satish; Jadhav, Sheetal; Parshetti, Ganesh K.; Govindwar, Sanjay P.

doi:10.1007/s12088-010-0001-5

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…Similar increases in activity in intracellular tyrosinase and lignin peroxidase have been reported for the biodegradation of Remazol Yellow RR [ 47 ], Reactive Black 5 [ 48 ], Remazol Orange 3R [ 49 ], Green HE4B [ 50 ], Brown 3REL [ 51 ], and Direct Red 5B [ 39 ]. Singh et al .…”

Section: Discussionsupporting

confidence: 60%

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products

Wang

Zhang

et al. 2022

Molecules

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The aims of this article were to investigate Bacillus safensis HL3 spore for its capacity to degrade and detoxify indigo carmine and to provide an effective biological agent for the treatment of isatin dye wastewater. Bacillus safensis HL3 spore was found to decolorize indigo carmine by 97% in the presence of acetosyringone within 2 h. Significantly increased activities of spore laccase, intracellular tyrosinase, and lignin peroxidase upon exposure to indigo carmine were observed. The results of RT–qPCR also showed that the expression of laccase gene was significantly increased. The spore has the ability to degrade indigo carmine through oxidization. Furthermore, the pathway by which indigo carmine is degraded was investigated using liquid chromatography–mass spectrometry analysis to identify the biodegradation products. A detailed pathway of indigo carmine degradation by bacterial spores was proposed for the first time. Toxicity tests indicated that the biodegradation products of indigo carmine are non-toxic to Nicotiana tabacum seeds and are less hazardous to human erythrocytes than the original dye. Indigo carmine is a typical recalcitrant dye and severely jeopardizes human health. The results demonstrate the utility of the spore from Bacillus safensis HL3 for the degradation of indigo carmine and simultaneous reduction of its toxicity.

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Section: Discussionsupporting

confidence: 60%

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products

Wang

Zhang

et al. 2022

Molecules

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“…The antibacterial action of RV 1 dye and degraded metabolites was studied using well diffusion method wherein inhibition zone surrounding the well represented the toxicity index. The phytotoxicity assessment of dye (500 and 1000 mg/l) as well as degraded metabolites was carried out on Pennisetum glacum and Vigna radiate [24]. Ten seeds of respective plants were germinated in small pots; daily supplemented with relevant contents (distilled water, dye, or degraded metabolites) to seeds and maintaining light (12 h) and temperature (30°C) in a controlled environment.…”

Section: Toxicity Analysis Of Degraded Metabolitesmentioning

confidence: 99%

Laccase-conjugated amino-functionalized nanosilica for efficient degradation of Reactive Violet 1 dye

Gahlout

Rudakiya

Gupte³

2017

Int Nano Lett

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Immobilization of enzyme with nanostructures enhances its ideal characteristics, which may allow the enzyme to become more stable and resistant. The present investigation deals with the formulation of laccase nanosilica conjugates to overcome the problems associated with its stability and reusability. Synthesized nanosilica and laccase nanoparticles were spherical shaped, with the mean size of 220 and 615 nm, respectively. Laccase nanoparticles had an optimum temperature of 55°C and pH 4.0 for the oxidation of ABTS. Laccase nanoparticle retained 79% of residual activity till 20th cycle. It also showed 91% of its initial activity at lower temperatures even after 60 days. Laccase nanoparticles were applied for Reactive Violet 1 degradation wherein 96.76% of decolourization was obtained at pH 5.0 and 30°C within 12 h. Toxicity studies on microbes and plants suggested that the degraded metabolites were less toxic than control dye. Thus, the method applied for immobilization increased storage stability and reusability of laccase, and therefore, it can be utilized for efficient degradation of azo dyes.

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“…The term 'biosorption' can be defined as the passive absorption of organic and inorganic species such as dye molecules and metal ions, by the microbial biomass (Banat et al, 1996). Many types of microbial biomasses such as bacterial (Kalme et al, 2010), microalgal (Wang et al, 2007Badr et al, 2016;Clark and Anliker, 1980), and fungal bomass (Fu and Viraraghavan, 2002), can act as an efficient biosorbent for toxic dye removal from industrial effluents.…”

Section: Introductionmentioning

confidence: 99%

Activated biomass of the green microalga Chlamydomonas variabilis as an efficient biosorbent to remove methylene blue dye from aqueous solutions

Moghazy

2019

WSA

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The raw and activated biomass of a green microalga, Chlamydomonas variabilis, were investigated as adsorbents for the removal of methylene blue (MB) dye from aqueous solutions. Chlamydomonas variabilis was isolated and cultivated to obtain a sufficient algal biomass. The collected biomass was first oven-dried and then activated by H2SO4. The results obtained showed that the optimum adsorption of MB occurred over 30 min of contact time at pH 7 and an biosorbent dose of 1.5 and 1.0 g·L−1 of dried biomass and activated biosorbent, respectively. Point of zero charge (pHpzc) was recorded at pH 6.8 and 6.9 for dried and activated biomass, respectively. The activated biomass was a more effective biosorbent than was the dried biomass: At a MB concentration of 82.4 mg·L−1, the minimum removal was greater than 98% using 1 g·L−1 activated biomass with a maximum adsorption capacity (qmax) of 115 mg·g−1, whereas at a MB concentration of 56.4 mg·L−1, the maximum removal did not exceed 80.8% using 1.5 g·L−1 raw biomass with a qmax of 18.3 mg·g−1. Furthermore, the Freundlich and Langmuir isotherm models of adsorption showed a better model fit when using activated biomass than when using raw biomass, with the former yielding R2 values greater than 0.9. The kinetic data suggest that the adsorption of MB follows the pseudo-second-order equation better than the pseudo-first-order one. This study demonstrates that the activated biomass of Chlamydomonas variabilis can be used as an effective biosorbent for the treatment of dye-containing wastewater streams.

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Biodegradation of Green HE4B: Co-substrate effect, biotransformation enzymes and metabolite toxicity analysis

Cited by 10 publications

References 22 publications

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products

The Biodegradation of Indigo Carmine by Bacillus safensis HL3 Spore and Toxicity Analysis of the Degradation Products

Laccase-conjugated amino-functionalized nanosilica for efficient degradation of Reactive Violet 1 dye

Activated biomass of the green microalga Chlamydomonas variabilis as an efficient biosorbent to remove methylene blue dye from aqueous solutions

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