Biodegradation of polyester polyurethane by Cladosporium sp. P7: Evaluating its degradation capacity and metabolic pathways

Liu, Jiawei; Qing-yuan, Zeng; Lei, Huirui; Xin, Kaiyuan; Xu, Anming; Wei, Ren; Li, Ding; Zhou, Jie; Dong, Weiliang; Jiang, Min

doi:10.1016/j.jhazmat.2023.130776

Cited by 37 publications

(13 citation statements)

References 35 publications

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“…Very recently, Liu et al 121 isolated 20 strains (namely, 9 fungi and 11 bacteria) and assessed their suitability for biodegrading a poly(1,4-butylene adipate)-based polyurethane. Among the different tested strains, Cladosporium sp.…”

Section: Polyester Polyurethane Coatingmentioning

confidence: 99%

Recycling of polyurethanes: where we are and where we are going

Rossignolo,

Malucelli,

Lorenzetti

2024

Green Chem.

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Section: Polyester Polyurethane Coatingmentioning

confidence: 99%

Recycling of polyurethanes: where we are and where we are going

Rossignolo,

Malucelli,

Lorenzetti

2024

Green Chem.

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“…The self-synthesized film was prepared by dissolving poly(1,4butylene adipate)-PU (PBA-PU) in chloromethane and then dried by volatilization. [22,23] The LB medium was used for expressing a recombinant enzyme using isopropyl-β-D-thiogalactoside (IPTG) as the inducer. [24] SDS-PAGE was performed to determine whether the recombinant enzymes were expressed, which were subsequently purified with Ni 2+ -NTA resin in a Biomolecular Liquid Chromatography System (AKTA design, GE Healthcare, US) using 0-300 mM imidazole in a buffer.…”

Section: Chemicals and Mediamentioning

confidence: 99%

Depolymerization of the polyester–polyurethane by amidase GatA250 and enhancing the production of 4,4′‐methylenedianiline with cutinase LCC

Xin,

Lu,

Zeng

et al. 2024

Biotechnology Journal

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Polyurethane (PU) is a complex polymer synthesized from polyols and isocyanates. It contains urethane bonds that resist hydrolysis, which decreases the efficiency of biodegradation. In this study, we first expressed the amidase GatA250, and then, assessed the enzymatic characterization of GatA250 and its efficiency in degrading the polyester‐PU. GatA250 degraded self‐synthesized thermoplastic PU film and postconsumption foam with degradation efficiency of 8.17% and 4.29%, respectively. During the degradation, the film released 14.8 µm 4,4′‐methylenedianiline (MDA), but 1,4‐butanediol (BDO) and adipic acid (AA) were not released. Our findings indicated that GatA250 only cleaved urethane bonds in PU, and the degradation efficiency was extremely low. Hence, we introduced the cutinase LCC, which possesses hydrolytic activity on the ester bonds in PU, and then used both enzymes simultaneously to degrade the polyester‐PU. The combined system (LCC‐GatA250) had higher degradation efficiency for the degradation of PU film (42.2%) and foam (13.94%). The combined system also showed a 1.80 time increase in the production of the monomer MDA, and a 1.23 and 3.62 times increase in the production of AA and BDO, respectively, compared to their production recorded after treatment with only GatA250 or LCC. This study provides valuable insights into PU pollution control and also proposes applicable solutions to manage PU wastes through bio‐recycling.

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“…In the next few years, it will be crucial to isolate the microbial enzymatic machinery involved in the degradation of less recalcitrant polyester PURs by using model PUR substrates and developing new specific assays (as presented by Liu et al [ 169 ]). This will allow for mimicking of the whole-organism biodegradation action using an enzymatic cocktail or a stepwise addition of enzymes.…”

Section: Biotechnological Systems Applied To Plastic Depolymerization...mentioning

confidence: 99%

Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives

Orlando

Molla

Castellani

et al. 2023

IJMS

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The accumulation of synthetic plastic waste in the environment has become a global concern. Microbial enzymes (purified or as whole-cell biocatalysts) represent emerging biotechnological tools for waste circularity; they can depolymerize materials into reusable building blocks, but their contribution must be considered within the context of present waste management practices. This review reports on the prospective of biotechnological tools for plastic bio-recycling within the framework of plastic waste management in Europe. Available biotechnology tools can support polyethylene terephthalate (PET) recycling. However, PET represents only ≈7% of unrecycled plastic waste. Polyurethanes, the principal unrecycled waste fraction, together with other thermosets and more recalcitrant thermoplastics (e.g., polyolefins) are the next plausible target for enzyme-based depolymerization, even if this process is currently effective only on ideal polyester-based polymers. To extend the contribution of biotechnology to plastic circularity, optimization of collection and sorting systems should be considered to feed chemoenzymatic technologies for the treatment of more recalcitrant and mixed polymers. In addition, new bio-based technologies with a lower environmental impact in comparison with the present approaches should be developed to depolymerize (available or new) plastic materials, that should be designed for the required durability and for being susceptible to the action of enzymes.

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Biodegradation of polyester polyurethane by Cladosporium sp. P7: Evaluating its degradation capacity and metabolic pathways

Cited by 37 publications

References 35 publications

Recycling of polyurethanes: where we are and where we are going

Recycling of polyurethanes: where we are and where we are going

Depolymerization of the polyester–polyurethane by amidase GatA250 and enhancing the production of 4,4′‐methylenedianiline with cutinase LCC

Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives

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