Biodeinking of waste papers using combinatorial fungal enzymes and subsequent production of butanol from effluent

Mondal, Subhadeep; Biswal, Divyajyoti; Pal, Kalyanbrata; Rakshit, Subham; Halder, Suman Kumar; Mandavgane, Sachin A.; Bera, Debabrata; Hossain, Maidul; Mondal, Keshab Chandra

doi:10.1016/j.biortech.2022.127078

Cited by 32 publications

(2 citation statements)

References 34 publications

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“…Finally, the upper phase was treated and injected into the HPLC for the analysis of ginsenosides. To obtain the optimal recovery concentration of ginsenosides, the effects of DES (Bet: EG, 2:1) concentration (28,30,32,34,36,38, and 40 wt %), K 2 HPO 4 concentration (60, 65, 70, 75, and 80 wt %), and extraction time (0.25, 0.5, 1, 2, 4, 6, 12, and 24 h) were investigated sequentially.…”

Section: Des-based Atpsmentioning

confidence: 99%

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High-Value Bioconversion of Ginseng Extracts in Betaine-Based Deep Eutectic Solvents for the Preparation of Deglycosylated Ginsenosides

Yang

Zhou

et al. 2023

Foods

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Deep eutectic solvents (DES), as a green alternative to traditional organic solvents in biocatalysis, not only activate proteins but even increase the efficiency of enzymatic reactions. Here, DES were used in a combinatorial enzyme-catalyzed system containing β-glucosidase BGLAt and β-galactosidase BGALAo to produce deglycosylated ginsenosides (De-g) from ginseng extracts (GE). The results showed that DES prepared with betaine and ethylene glycol (molar ratio, 1:2) could significantly stimulate the activity of the combinatorial enzymes as well as improve the acid resistance and temperature stability. The DES-based combinatorial enzyme-catalyzed system could convert 5 g of GE into 1.24 g of De-g (F1, F2, 20 (S)-PPT, and CK) at 24 h, which was 1.1 times that of the buffer sample. As confirmed by the spectral data, the changes in the conformations of the combinatorial enzymes were more favorable for the binding reaction with the substrates. Moreover, the constructed DES-based aqueous two-phase system enabled the recovery of substantial amounts of DES and De-g from the top phase. These results demonstrated that DES shows great application as a reaction solvent for the scale-up production of De-g and provide insights for the green extraction of natural products.

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Section: Des-based Atpsmentioning

confidence: 99%

“…It is well known that the optimum pH and temperature could make the enzymatic performance more effective [38,39]. Therefore, the effects of DES on the optimal pH and temperature of BGLAt and BGALAo were initially investigated.…”

Section: Effects Of Des (Bet: Eg 2:1) On Biocatalystsmentioning

confidence: 99%