Biodentine Inhibits the Initial Microbial Adhesion of Oral Microbiota In Vivo

Abstract

This study aimed to evaluate the in vivo initial microbial adhesion of oral microorganisms on the biomaterial Biodentine compared to MTA and AH Plus. Cylindrical samples of the materials were prepared, and dentin slabs served as a control. An individual intraoral lower jaw splint served as a carrier for the samples and was worn by six volunteers. The specimens were worn for 120 min. Adherent bacteria were quantified by determining the colony-forming units (CFUs), while the visualization and quantification of total adherent microorganisms were facilitated by using DAPI and live/dead staining combined with fluorescence microscopy. Bovine dentin had a significantly higher number of aerobic CFUs compared to Biodentine (p = 0.017) and MTA (p = 0.013). The lowest amounts of DAPI-stained adherent microorganisms were quantified for Biodentine (15% ± 9%) and the control (18% ± 9%), while MTA showed the highest counts of initially adherent microorganisms (38% ± 10%). Significant differences were found for MTA and Biodentine (p = 0.004) as well as for MTA and the control (p = 0.021) and for AH Plus and the control (p = 0.025). Biodentine inhibited microbial adherence, thereby yielding an antimicrobial effectivity similar to that of MTA.

Comparative Evaluation of a New Tricalcium Silicate Cement With Calcium Hydroxide as Direct Pulp Capping Agents: A Clinical Study

“…The main characteristics of this product are the advent of reactive dentin to preserve the vitality of the pulp, complete remineralization with the development of a calcific barrier, antimicrobial characteristics owing to an elevated pH level of 12.5, natural micro-mechanical anchorage for improved sealing with no need for surface treatment, comparable mechanical characteristics and behavior as human dentin, and an aluminum radiopacity of 3.5 mm, which allows for easy short-and long-term monitoring. Additionally, it can be used in a single visit as a base or alternative for dentin under permanent restorations such as composites for vital pulp therapy [14,15].…”

Optimizing the use of low-frequency ultrasound for bacterial detachment of in vivo biofilms in dental research—a methodological study

“…Since initial microbial colonization takes place within minutes, many studies focus on biofilms due to their constant presence in the oral cavity [ 20 , 21 ]. Various samples, such as dentures [ 22 ], material samples [ 23 ], dentin slabs [ 24 ], or enamel slabs, have been examined through sonication [ 25 , 26 ]. The sonicate was used to determine viable bacteria [ 22 – 25 ] or for subsequent PCR analysis [ 26 ].…”

“…The sonicate was used to determine viable bacteria [ 22 – 25 ] or for subsequent PCR analysis [ 26 ]. Splint systems are often used to collect in vivo biofilm samples [ 24 – 30 ], since in vitro samples differ significantly [ 31 ]. Areas of oral biofilm research include dental material research [ 24 ], development of methods [ 28 ], new anti-caries strategies [ 32 ], characterization of the microbiota on dentures [ 22 ], and research on new antimicrobial agents [25, 27].…”

“…The duration of ultrasound treatment varied greatly, ranging from 20 s [ 30 ] to 20 min [ 26 ]. Most studies utilized a simple sonication of 1 to 5 min [ 22 , 24 , 25 , 27 – 29 , 36 , 37 ], except for one study that used repeated sonication [ 23 ]. The frequencies and intensities of ultrasound used were usually not specified.…”