Biodiversity conservation and conservation biotechnology tools

Reed, Barbara M.; Sarasan, Viswambharan; Kane, Michael E.; Bunn, Eric; Pence, Valerie C.

doi:10.1007/s11627-010-9337-0

Cited by 170 publications

(132 citation statements)

References 17 publications

(13 reference statements)

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“…Despite the progress of plant conservation through the use of in situ and ex situ protective strategies in botanical gardens and seed banks, new approaches are needed for some of the most endangered species. Ex situ seed conservation strategies, such as in vitro storage and cryopreservation, can complement in situ strategies and ensure the biodiversity preservation of species whose habitats are very threatened (Reed et al, 2011). The establishment of a genetic bank through seed storage is extremely important, both for wild species, that carry their own characteristics, and for breed species (Yam et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Hosomi¹,

Souza²,

Custódio³

et al. 2017

Aust J Crop Sci

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The evaluation of seed physiological quality can help taking quick decision about the use or disposal of lots for conservation. The tetrazolium test is one of the most traditional in the analysis of seeds quality. Optional preconditioning procedures have improved the efficiency of this test. This work compared the efficiency of different preconditioning procedures, the buffered solution, concentrations and exposure time to tetrazolium solution in assessing the quality of orchid seeds of Cattleya labiata and Cattleya tigrina lots. The analysis was made through magnification of digital images. For preconditioning evaluation, two seed lots were aliquoted and submitted to different treatments: 10% sucrose solution, 10% glucose solution and water at room temperature for 24 hours. After preconditioning, seeds were immersed in tetrazolium solutions -prepared with buffer or water -at concentrations of 0.1, 0.5 and 1.0% for periods of 3, 12 and 24 hours, at 40 °C, in a water bath, in the dark. The experiment design was arranged in a factorial 4x3x3x2 and the mean comparison was made by Tukey test at 5% probability. Tetrazolium test in preconditioned orchid seeds allowed better visualization of red viable seeds. Tetrazolium test in orchid seeds preconditioned in glucose 10% solution or sucrose 10% solution was more effective for viable seeds visualization. Diluting tetrazolium salt in buffered solution or water made no difference for the results. Maintaining seeds in contact with tetrazolium 1% solution for 24 hours provided the higher viability values for both seed lots.

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Section: Introductionmentioning

confidence: 99%

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Hosomi¹,

Souza²,

Custódio³

et al. 2017

Aust J Crop Sci

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“…The most important aspects of this protection are the development of efficient regeneration systems for many endangered species, that is micropropagation, and the protection of the morphogenetic potential of cell cultures, tissue or organs (Rybczyński and Mikuła 2006). Although standard in vitro propagation methods are available, endangered species may have unusual growth requirements and thus may require modified procedures for in vitro culture (Reed et al 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitro slow growth storage of Senecio macrophyllus shoots

2015

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Advances in biotechnology, especially in the field of in vitro culture techniques, led to the development of procedures that can be used as an excellent tool in plant conservation. The present study describes in vitro conservation of Senecio macrophyllus by slow-growth storage. Various sugar treatments, concentration of abscisic acid (ABA), light intensity and type of containers were tested. Viability and proliferation rate of shoots were evaluated 4 weeks after regrowth. The results obtained showed that polycarbonate boxes were a better type of containers for storage of S. macrophyllus shoots than glass vessels. Light negatively affected culture viability, multiplication rate and rooting response. In the case of Senecio macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on medium without ABA. Shoots of S. macrophyllus were effectively stored for 6 months. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization.

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“…The in-vitro techniques for conserving plant biodiversity include shoot apical or axillary-meristem-based micro-propagation, somatic embryogenesis, cell culture technologies and embryo rescue techniques, as well as a range of in-vitro cold storage and cryopreservation (Engelmann 1997, Reed et al 2011, in-vitro collection, pathogen free plantlet maintenance and efficient germplasms exchange.…”

Section: A In-vitro Tissue Conservationmentioning

confidence: 99%

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

Joshi

2017

J. Nep. Agric. Res. Counc.

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Agricultural biodiversity is the basis of human life and food security. Nepal with 577 cultivated species possesses huge diversity at varietal as well as landrace levels. In most agricultural crops the rapid genetic erosion due to several reasons is a common phenomenon. Thus, considering the importance of agricultural biodiversity declared by Convention on Biological Diversity for sustainable food production, National Agriculture Genetic Resources Center (NAGRC) has been established for conservation and sustainable utilization of agricultural biodiversity. This paper thus delineates the application of biotechnological tools adopted by NAGRC for effective and efficient conservation and use of agricultural plant genetic resources (APGRs). Among the adopted technologies, tissue bank using shoot tip culture of vegetatively propagating and recalcitrant crops eg potato, sugarcane, banana, sweet potato, etc are in function. Under the molecular marker technology, currently random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers have been used for developing DNA profiles, identifying duplicates in the collections, assessing genetic diversity and screening accessions against economic traits. DNA bank has also been created for storing DNA of indigenous crops and these DNA can be accessed for research and study. Genotypic database has been developed for chayote, finger millet, wheat and maize for identification and selection of the accessions.

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Biodiversity conservation and conservation biotechnology tools

Cited by 170 publications

References 17 publications

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

In vitro slow growth storage of Senecio macrophyllus shoots

Biotechnology for Conservation and Utilization of Agricultural Plant Genetic Resources in Nepal

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