Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well

Abstract

Cancer cell lines are important tools for anticancer drug research and assessment. Impedance measurements can provide valuable information about cell viability in real time. This work presents the proof-of-concept development of a bioelectrical, impedance-based analysis technique applied to four adherent mammalian cancer cells lines immobilized in a three-dimensional (3D) calcium alginate hydrogel matrix, thus mimicking in vivo tissue conditions. Cells were treated with cytostatic agent5-fluoruracil (5-FU). The cell lines used in this study were SK-N-SH, HEK293, HeLa, and MCF-7. For each cell culture, three cell population densities were chosen (50,000, 100,000, and 200,000 cells/100 μL). The aim of this study was the extraction of mean impedance values at various frequencies for the assessment of the different behavior of various cancer cells when 5-FU was applied. For comparison purposes, impedance measurements were implemented on untreated immobilized cell lines. The results demonstrated not only the dependence of each cell line impedance value on the frequency, but also the relation of the impedance level to the cell population density for every individual cell line. By establishing a cell line-specific bioelectrical behavior, it is possible to obtain a unique fingerprint for each cancer cell line reaction to a selected anticancer agent.

Evaluation of Cancer Cell Lines by Four-Point Probe Technique, by Impedance Measurements in Various Frequencies

“…Bioelectric profiling of cancer cell responses to chemotherapeutic agents is an emerging research field, as indicated by another report [ 32 ] by our group of authors where impedance spectroscopy was applied in order to study interactions between the anticancer cytostatic agent 5-fluoruracil (5-FU) and four adherent mammalian cancer cells lines were immobilized in a three-dimensional (3D) calcium alginate hydrogel matrix, thus mimicking in vivo tissue conditions.…”

“…Human neuroblastoma cell line SK-N-SH (ATCC ® HTB-11™), HeLa cervical cancer cells (ATCC ® CRM-CCL-2™), triple positive breast cell line MCF-7 (ATCC ® HTB-22™), and triple negative MDA-MB-231 (ATCC ® HTB-26™) were cultured in an incubator at 37 °C, 5% CO 2 as described previously [ 32 ]. Doxorubicin hydrochloride (>98.0%) (DOX) (Fluka Chemie GmbH, Buchs, Switzerland) was initially dissolved in dimethylsulfoxide (DMSO).…”

Farm or Lab? A Comparative Study of Oregano’s Leaf and Callus Volatile Isolates Chemistry and Cytotoxicity

“…The cells were cultured in T-75 flasks (Sarstedt AG & Co. KG, Nümbrecht, Germany) in an appropriate incubator with humidified atmosphere at 37 °C and 5% CO 2 . Regular subcultures (twice a week) were conducted at a 1:10 ratio [ 106 ] and the adherent cells were detached from the flask by trypsinization using trypsin/ethylenediaminetetraacetic acid (EDTA) for 10 min at 37 °C.…”

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

“…Our research group has previously employed this approach to characterize interactions between breast cancer cells and different chemotherapeutical agents [24,25] as well as neuronal cells and neurotransmitters [26]. In the present study, we compared the Salmonella infection progression in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with a different bacterial multiplicity of infection (MOI) by assessing the alterations of frequencies recordings with impedance spectroscopy measurements.…”