Bioengineered Trachea with Fibroblasts in a Rabbit Model

Okano, Wataru; Nomoto, Yukio; Wada, Ikuo; Kobayashi, Ken; Miyake, Masakazu; Nakamura, Tatsuo; Omori, Koichi

doi:10.1177/000348940911801109

Cited by 28 publications

(15 citation statements)

References 19 publications

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“…Okano et al reported that measurement of the average thickness of the regenerated epithelium showed that the tracheal fibroblasts accelerated regeneration of the tracheal epithelium [7]. In the present study, the difference in the thickness of the epithelial layer was observed at 7, 14, and 28 days post-implantation.…”

Section: Discussionsupporting

confidence: 55%

“…In response to this problem for regenerative medicine of the trachea, our group developed a collagen sponge scaffold with cultured fibroblasts, adipose tissue-derived stem cells, and induced pluripotent stem cells into rat or rabbit tracheal defects and reported the effective epithelial regeneration [6][7][8][9][10][11]. To promote regeneration of the tracheal epithelium without a cell source, we developed a collagen vitrigel scaffold possessing a smooth surface to facilitate epithelial cell migration after implantation [12].…”

Section: Introductionmentioning

confidence: 99%

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Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Tada

Takezawa

Tani

et al. 2012

Acta Oto-Laryngologica

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Section: Discussionsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Tada

Takezawa

Tani

et al. 2012

Acta Oto-Laryngologica

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“…7 To promote regeneration of the tracheal epithelium, our group has studied the effects of fibroblasts, adipose-derived stem cells, and basic-fibroblast growth factor in artificial grafts and shown that they shorten the time for epithelialization and cilia formation. [8][9][10][11][12][13][14] Collagen scaffolds are widely used in regenerative medicine because of their high biocompatibility, but no studies have examined the most suitable structure of collagen scaffold for tracheal regeneration.…”

Section: Introductionmentioning

confidence: 99%

Effect of Structural Differences in Collagen Sponge Scaffolds on Tracheal Epithelium Regeneration

Nakaegawa

Nakamura

Tada

et al. 2015

Ann Otol Rhinol Laryngol

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Objective: We developed an in situ regeneration-inducible artificial trachea composed of a porcine collagen sponge and polypropylene framework and used it for tracheal reconstruction. In the present study, collagen sponges with different structures were prepared from various concentrations of collagen solutions, and their effect on the regeneration of tracheal epithelium was examined. Methods: Collagen sponges were prepared from type I and III collagen solutions. The structures of the sponges were analyzed using scanning electron microscopy (SEM). Artificial tracheae, which were formed using the collagen sponges with different structures, were implanted into rabbits, and regeneration of the tracheal epithelium on the artificial tracheae was evaluated by SEM analysis and histological examination. Results: The SEM analysis showed that collagen sponges prepared from 0.5% and 1.0% collagen solutions had a porous structure. However, the sponges prepared from a 1.5% collagen solution had a nonporous structure. After implantation of artificial tracheae prepared from 0.5% and 1.0% collagen solutions, their luminal surfaces were mostly covered with epithelium within 14 days. However, epithelial reorganization occurred later on artificial tracheae prepared from the 1.5% collagen solution. Conclusion:Collagen sponges with a porous structure are suitable for regeneration of the tracheal epithelium in our artificial trachea.

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“…63 Differentiated targets for reepithelialization have reported success with tracheal epithelial cells, tracheal fibroblasts, gingival fibroblasts, buccal mucosa, and skin. 3,8,26,28,29,49,64,65 …”

Section: Discussionmentioning

confidence: 99%

“…However, complete epithelialization has been observed in constructs using bone marrow–derived mesenchymal stem cells (BM-MSC), autologous epithelial cells, buccal mucosal graft, or tracheal fibroblasts. 6,8,29,49 It is unclear if respiratory epithelialization represents survival of donor epithelium or regeneration by the recipient modulated by a paracrine effect from seeded cells. However, epithelial migration from the anastomotic sites has been demonstrated in some studies, suggesting recipient derived neotissue.…”

Section: Discussionmentioning

confidence: 99%

Clinical Translation of Tissue Engineered Trachea Grafts

Chiang

Pepper

Best

et al. 2016

Ann Otol Rhinol Laryngol

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Objective To provide a state-of-the-art review discussing recent achievements in tissue engineered tracheal reconstruction. Data Sources and Review Methods A structured PubMed search of the current literature up to and including October 2015. Representative articles that discuss the translation of tissue engineered tracheal grafts (TETG) were reviewed. Conclusions The integration of a biologically compatible support with autologous cells has resulted in successful regeneration of respiratory epithelium, cartilage, and vascularization with graft patency, although the optimal construct composition has yet to be defined. Segmental TETG constructs are more commonly complicated by stenosis and delayed epithelialization when compared to patch tracheoplasty. Implications for Practice The recent history of human TETG recipients represents revolutionary proof of principle studies in regenerative medicine. Application of TETG remains limited to a compassionate use basis; however, defining the mechanisms of cartilage formation, epithelialization, and refinement of in vivo regeneration will advance the translation of TETG from the bench to the bedside.

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Bioengineered Trachea with Fibroblasts in a Rabbit Model

Abstract: This study indicated that fibroblasts had a stimulatory effect that hastened regeneration of the epithelium in large tracheal defects.

Cited by 28 publications

References 19 publications

Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Effect of Structural Differences in Collagen Sponge Scaffolds on Tracheal Epithelium Regeneration

Clinical Translation of Tissue Engineered Trachea Grafts

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