Bioethanol production from wheat straw by phosphoric acid plus hydrogen peroxide (PHP) pretreatment via simultaneous saccharification and fermentation (SSF) at high solid loadings

Qiu, Jingwen; Tian, Dong; Shen, Fei; Hu, Jinguang; Zeng, Yongmei; Yang, Gang; Zhang, Yanzong; Deng, Shihuai; Zhang, Jing

doi:10.1016/j.biortech.2018.08.009

Cited by 66 publications

(15 citation statements)

References 34 publications

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“…The results indicate diversity of hemicelluloses among herbaceous and woody plant SCWs, corroborating the idea that the molecular heterogeneity of hemicelluloses plays a key role in modulating the interactions with cellulose microfibrils [45] and the occurrence of covalent linkages with lignin [12]. The composition of the crop residues was also determined (Figure 4), and the derived contents of cellulose, hemicelluloses, and lignin were in the range reported in previous studies [46][47][48][49][50][51][52][53][54]. While slightly reduced levels of hemicelluloses were observed in the herbaceous residues of WS (Figure 4A), SG (Figure 4B), and RS (Figure 4C), hemicellulase pretreatment had no significant effect on the level of hemicelluloses in the woody residue of AB (Figure 4D).…”

Section: Hemicellulase Pretreatment Modulated Hydrolytic Preferences Of Pathogenic Fungi For Biomass Typesupporting

confidence: 87%

Comparative Analysis of Herbaceous and Woody Cell Wall Digestibility by Pathogenic Fungi

et al. 2021

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Fungal pathogens have evolved combinations of plant cell-wall-degrading enzymes (PCWDEs) to deconstruct host plant cell walls (PCWs). An understanding of this process is hoped to create a basis for improving plant biomass conversion efficiency into sustainable biofuels and bioproducts. Here, an approach integrating enzyme activity assay, biomass pretreatment, field emission scanning electron microscopy (FESEM), and genomic analysis of PCWDEs were applied to examine digestibility or degradability of selected woody and herbaceous biomass by pathogenic fungi. Preferred hydrolysis of apple tree branch, rapeseed straw, or wheat straw were observed by the apple-tree-specific pathogen Valsa mali, the rapeseed pathogen Sclerotinia sclerotiorum, and the wheat pathogen Rhizoctonia cerealis, respectively. Delignification by peracetic acid (PAA) pretreatment increased PCW digestibility, and the increase was generally more profound with non-host than host PCW substrates. Hemicellulase pretreatment slightly reduced or had no effect on hemicellulose content in the PCW substrates tested; however, the pretreatment significantly changed hydrolytic preferences of the selected pathogens, indicating a role of hemicellulose branching in PCW digestibility. Cellulose organization appears to also impact digestibility of host PCWs, as reflected by differences in cellulose microfibril organization in woody and herbaceous PCWs and variation in cellulose-binding domain organization in cellulases of pathogenic fungi, which is known to influence enzyme access to cellulose. Taken together, this study highlighted the importance of chemical structure of both hemicelluloses and cellulose in host PCW digestibility by fungal pathogens.

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Section: Hemicellulase Pretreatment Modulated Hydrolytic Preferences Of Pathogenic Fungi For Biomass Typesupporting

confidence: 87%

Comparative Analysis of Herbaceous and Woody Cell Wall Digestibility by Pathogenic Fungi

et al. 2021

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“…When cellulase was applied at very low loading, the enzymatic hydrolysis of DryPB corn stover was limited, but lipid production at 37 • C could benefit from higher hydrolytic activity of cellulase. Similar results have been observed for cellulosic ethanol fermentation [32]. At a high cellulase loading, enzymatic hydrolysis secured sugar release such that the lipid production process was depending on yeast cell activity.…”

Section: Lipid Production Using Presslp Processsupporting

confidence: 85%

Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process

Dai

Shen

et al. 2019

Energies

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Dry acid pretreatment and biodetoxification (DryPB) has been considered as an advanced technology to treat lignocellulosic materials for improved downstream bioconversion. In this study, the lipid production from DryPB corn stover was investigated by the oleaginous yeast Rhodosporidium toruloides using a new process designated prehydrolysis followed by simultaneous saccharification and lipid production (PreSSLP). The results found that prehydrolysis at 50 °C and then lipid production at 30 °C improved lipid yield by more than 17.0% compared with those without a prehydrolysis step. The highest lipid yield of 0.080 g/g DryPB corn stover was achieved at a solid loading of 12.5%. The fatty acid distribution of lipid products was similar to those of conventional vegetable oils that are used for biodiesel production. Our results suggested that the integration of DryPB process and PreSSLP process can be explored as an improved technology for microbial lipid production from lignocellulosic materials.

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“…Among various chemical methods, solvent-based pretreatments have been recognized to be effective in disrupting the hydrogen bonding network of the cellulosic component through the dissolution–regeneration mechanism [ 13 ]. Previous work has shown that the concentrated H 3 PO 4 plus H 2 O 2 (PHP) pretreatment exhibited good performance on removing hemicellulose and lignin fractions from lignocellulose biomass through an aggressive oxidation process while enhancing the subsequent enzymatic saccharification of cellulose component, even at high solids loadings [ 14 ]. Whereas, concentrated H 3 PO 4 or H 2 O 2 alone showed much lower ability in biomass deconstruction and delignification [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…At the same time, the residual phosphorus in the wastewater could be removed using biochar adsorption to reduce phosphorus emissions [ 6 ]. At the optimized conditions, rather high cellulose hydrolysis and bioethanol conversion were achieved using simultaneous saccharification and fermentation process (SSF), e.g., 15.5 g ethanol was produced from 100 g wheat straw at rather high solid loadings [ 14 , 17 ]. It has been evidenced the strong oxidation reaction that occurred in PHP pretreatment system played an important role in biomass deconstruction and delignification to ease cellulose hydrolysis [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Self-generated peroxyacetic acid in phosphoric acid plus hydrogen peroxide pretreatment mediated lignocellulose deconstruction and delignification

Tian

Chen

Shen

et al. 2021

Biotechnol Biofuels

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Background Peroxyacetic acid involved chemical pretreatment is effective in lignocellulose deconstruction and oxidation. However, these peroxyacetic acid are usually artificially added. Our previous work has shown that the newly developed PHP pretreatment (phosphoric acid plus hydrogen peroxide) is promising in lignocellulose biomass fractionation through an aggressive oxidation process, while the information about the synergistic effect between H3PO4 and H2O2 is quite lack, especially whether some strong oxidant intermediates is existed. In this work, we reported the PHP pretreatment system could self-generate peroxyacetic acid oxidant, which mediated the overall lignocellulose deconstruction, and hemicellulose/lignin degradation. Results The PHP pretreatment profile on wheat straw and corn stalk were investigated. The pathways/mechanisms of peroxyacetic acid mediated-PHP pretreatment were elucidated through tracing the structural changes of each component. Results showed that hemicellulose was almost completely solubilized and removed, corresponding to about 87.0% cellulose recovery with high digestibility. Rather high degrees of delignification of 83.5% and 90.0% were achieved for wheat straw and corn stalk, respectively, with the aid of peroxyacetic acid oxidation. A clearly positive correlation was found between the concentration of peroxyacetic acid and the extent of lignocellulose deconstruction. Peroxyacetic acid was mainly self-generated through H2O2 oxidation of acetic acid that was produced from hemicellulose deacetylation and lignin degradation. The self-generated peroxyacetic acid then further contributed to lignocellulose deconstruction and delignification. Conclusions The synergistic effect of H3PO4 and H2O2 in the PHP solvent system could efficiently deconstruct wheat straw and corn stalk lignocellulose through an oxidation-mediated process. The main function of H3PO4 was to deconstruct biomass recalcitrance and degrade hemicellulose through acid hydrolysis, while the function of H2O2 was to facilitate the formation of peroxyacetic acid. Peroxyacetic acid with stronger oxidation ability was generated through the reaction between H2O2 and acetic acid, which was released from xylan and lignin oxidation/degradation. This work elucidated the generation and function of peroxyacetic acid in the PHP pretreatment system, and also provide useful information to tailor peroxide-involved pretreatment routes, especially at acidic conditions. Graphical abstract

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Bioethanol production from wheat straw by phosphoric acid plus hydrogen peroxide (PHP) pretreatment via simultaneous saccharification and fermentation (SSF) at high solid loadings

Cited by 66 publications

References 34 publications

Comparative Analysis of Herbaceous and Woody Cell Wall Digestibility by Pathogenic Fungi

Comparative Analysis of Herbaceous and Woody Cell Wall Digestibility by Pathogenic Fungi

Microbial Lipid Production from Corn Stover by the Oleaginous Yeast Rhodosporidium toruloides Using the PreSSLP Process

Self-generated peroxyacetic acid in phosphoric acid plus hydrogen peroxide pretreatment mediated lignocellulose deconstruction and delignification

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