Biofilm characteristics and transcriptomic analysis of Haemophilus parasuis

Jiang, Ruijiao; Xiang, Ming; Chen, Wanting; Zhang, Pengfei; Wu, Xulong; Zhu, Guoqiang; Tu, Teng; Jiang, Dike; Yao, Xueping; Luo, Yan; Yang, Zexiao; Chen, Di-Shi; Wang, Yin

doi:10.1016/j.vetmic.2021.109073

Cited by 15 publications

(8 citation statements)

References 56 publications

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“…Herein, the four genes of dltA (ILP77_RS14440), dltB (ILP77_RS14435), dltC (ILP77_RS14430), and dltD (ILP77_RS14425) were all significantly downregulated, which maybe lead to the negative effects of decreasing cell wall stabilization, inhibiting MRSA growth and bacterial biofilm formation. ArtQ encodes an arginine ABC transporter permease and is closely related to bacterial colonization and adhesion ( Jiang et al, 2021 ). Some studies have also found that mutation or deletion of artQ inhibited the intake of antibiotics, leading to the increased bacterial resistance to antibiotics ( Smith et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and antibiofilm activities of protocatechualdehyde and its synergy with ampicillin against methicillin-resistant Staphylococcus aureus

Wang,

Liu,

Song

et al. 2024

Front. Microbiol.

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Protocatechualdehyde (PA) is a phenolic acid present in many plants and has many biological activities. Herein, the antagonistic effects and the action mechanism of PA against methicillin-resistant Staphylococcus aureus (MRSA) were studied. The results showed that PA had both significant antibacterial and anti-biofilm activities against MRSA. Additionally, PA had synergy with ampicillin against MRSA. It was elucidated that PA was prominent in destroying cell membranes, increasing cell membrane permeability and intracellular ROS production, thus leading to bacterial cell damage. Transcriptome analysis showed that PA disrupts many physiological pathways, including increasing cell membrane permeability, inhibiting biofilm formation, decreasing resistance to antimicrobial agents, and impairing DNA replication. Finally, the antimicrobial preservation test showed that PA could inhibit the growth of MRSA and prevent the corruption of beef. In summary, PA is an effective natural antibacterial substance and has a good application potential in food preservation, even in tackling antibiotic resistance problems.

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Section: Resultsmentioning

confidence: 99%

Antibacterial and antibiofilm activities of protocatechualdehyde and its synergy with ampicillin against methicillin-resistant Staphylococcus aureus

Wang,

Liu,

Song

et al. 2024

Front. Microbiol.

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“…Generally, based on numerous transcriptional studies, the genes controlled by QS can be classified into four categories based on their biological functions [ 152 , 160 , 161 ]. The first group includes genes involved in cell life and growth; the second group includes genes controlling the behaviour of cells in the environment; the third group includes genes associated with HGT; and the fourth group includes genes whose expression is correlated with the synthesis of virulence factors [ 152 , 160 ].…”

Section: Molecular Mechanisms Of the Formation And Functioning Of Bac...mentioning

confidence: 99%

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Juszczuk-Kubiak

2024

IJMS

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One of the key mechanisms enabling bacterial cells to create biofilms and regulate crucial life functions in a global and highly synchronized way is a bacterial communication system called quorum sensing (QS). QS is a bacterial cell-to-cell communication process that depends on the bacterial population density and is mediated by small signalling molecules called autoinducers (AIs). In bacteria, QS controls the biofilm formation through the global regulation of gene expression involved in the extracellular polymeric matrix (EPS) synthesis, virulence factor production, stress tolerance and metabolic adaptation. Forming biofilm is one of the crucial mechanisms of bacterial antimicrobial resistance (AMR). A common feature of human pathogens is the ability to form biofilm, which poses a serious medical issue due to their high susceptibility to traditional antibiotics. Because QS is associated with virulence and biofilm formation, there is a belief that inhibition of QS activity called quorum quenching (QQ) may provide alternative therapeutic methods for treating microbial infections. This review summarises recent progress in biofilm research, focusing on the mechanisms by which biofilms, especially those formed by pathogenic bacteria, become resistant to antibiotic treatment. Subsequently, a potential alternative approach to QS inhibition highlighting innovative non-antibiotic strategies to control AMR and biofilm formation of pathogenic bacteria has been discussed.

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“…In previous studies, G. parasuis had the ability to form biofilm ( Jin et al, 2006 ). The part gene expression levels of G. parasuis changed significantly before and after the formation of the biofilm ( Jiang R. et al, 2021 ). For instance, the cytoplasmic heme-binding protein HutX, characterized by transporting the heme-degrading enzyme HutZ, can affect the formation of the biofilm and result in chronic infection of G. parasuis ( Sekine et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…This may provide a clue to the pathogenic mechanism. Glycogen operon protein GlgX, playing an essential role in starch and sucrose metabolism pathways, can maintain the structural integrity of the biofilm, suggesting that metabolism-related genes are similarly closely related to biofilm formation ( Sheppard and Howell, 2016 ; Jiang R. et al, 2021 ). However, these genes related to biofilm formation have still been explored.…”

Section: Introductionmentioning

confidence: 99%

Pan-genome wide association study of Glaesserella parasuis highlights genes associated with virulence and biofilm formation

et al. 2023

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Glaesserella parasuis is a gram-negative bacterium that causes fibrotic polyserositis and arthritis in pig, significantly affecting the pig industry. The pan-genome of G. parasuis is open. As the number of genes increases, the core and accessory genomes may show more pronounced differences. The genes associated with virulence and biofilm formation are also still unclear due to the diversity of G. parasuis. Therefore, we have applied a pan-genome-wide association study (Pan-GWAS) to 121 strains G. parasuis. Our analysis revealed that the core genome consists of 1,133 genes associated with the cytoskeleton, virulence, and basic biological processes. The accessory genome is highly variable and is a major cause of genetic diversity in G. parasuis. Furthermore, two biologically important traits (virulence, biofilm formation) of G. parasuis were studied via pan-GWAS to search for genes associated with the traits. A total of 142 genes were associated with strong virulence traits. By affecting metabolic pathways and capturing the host nutrients, these genes are involved in signal pathways and virulence factors, which are beneficial for bacterial survival and biofilm formation. This research lays the foundation for further studies on virulence and biofilm formation and provides potential new drug and vaccine targets against G. parasuis.

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Biofilm characteristics and transcriptomic analysis of Haemophilus parasuis

Cited by 15 publications

References 56 publications

Antibacterial and antibiofilm activities of protocatechualdehyde and its synergy with ampicillin against methicillin-resistant Staphylococcus aureus

Antibacterial and antibiofilm activities of protocatechualdehyde and its synergy with ampicillin against methicillin-resistant Staphylococcus aureus

Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

Pan-genome wide association study of Glaesserella parasuis highlights genes associated with virulence and biofilm formation

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