Biofilm formation by Staphylococcus aureus from food contact surfaces in a meat-based broth and sensitivity to sanitizers

Abstract

This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 × 2 cm) when cultivated in a meat-based broth at 28 and 7 °C. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered on polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacity to adhere and form biofilm on polypropylene and stainless steel surfaces under different growth conditions. Moreover, the cells in biofilm matrix were resistant for total removal when submitted to the exposure to sanitizers.

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

“…Moreover, the ability of S. aureus isolated from industrial surfaces to form biofilm depends on the environmental conditions. The hygienic condition of food handlers is also associated with the propagation of S. aureus biofilm producer (Gutiérrez et al 2012, Souza et al 2014, Di Ciccio et al 2015. Several authors have reported E. faecalis strains isolated from food samples with ability to form biofilms (Medeiros et al 2014, Jahan and Holley 2014, Fernandes et al 2015.…”

Contaminantes bacterianos y perfil de susceptibilidad del semen porcino en centros de recogida em Brasil

“…Corroborando esta hipótesis, en el cultivo de células en caldo de soja tríptico a temperaturas inferiores a las óptimas (20, 25 y 30°C), Rode et al (21) encontraron la mayor capacidad de fijación de S. aureus en poliestireno. De Souza et al (22) observaron que las cepas de S. aureus se adhirieron en grandes cantidades independientemente del tipo de superficie evaluada y de la temperatura de incubación (7 o 28ºC). Por lo tanto, nuestros resultados sugieren la necesidad de mejores procesos de inactivación o eliminación de agentes contaminantes en las superficies de suppression of motility, changes in morphology, and fertilizing capacity.…”

Detection of exopolysaccharide production and biofilm-related genes in Staphylococcus spp. isolated from a poultry processing plant

“…The assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers such as sodium hypochlorite and peracetic acid (Souza et al, 2014;Jerônimo et al, 2012).…”

“…Thus, it is assumed that cell aggregates or biofilm formation by S. aureus can be a potential risk for food cross-contamination (Souza et al, 2014).…”

“…Understanding the processes of biofilm formation by S. aureus can facilitate the study on the ability of wild strains to adhere and to form biofilms when they are exposed to conditions simulating the environments found in food processing plants, and it allows a better definition of control strategies (Souza et al, 2014). PCR analysis only reveals the genetic predisposition for biofilm formation and expression of ica genes; thus, the real biofilm formation must be confirmed by additional phenotypic methods (Melo et al, 2013).…”