Biofilm forming potentiality of Escherichia coli isolated from bovine endometritis and their antibiotic resistance profiles

Abstract

Objective: The objectives of this study were to determine the biofilm-forming capability and antimicrobial susceptibility of Escherichia coli recovered from bovine endometritis samples. Materials and Methods: A total of 120 uterine specimens were collected from cows suffering from endometritis for bacteriological examination. Antimicrobial susceptibility testing was carried out for all isolated E. coli by using the disc diffusion method. The isolates were phenotypically studied for biofilm-forming ability by cultivation on yeast extract -casamino acids Congo red agar (CRA). Some randomly selected isolates were chosen for the molecular identification of some virulence and resistance genes. Results: A total of 58(48.3%) E. coli isolates could be isolated from the 120 samples. Antimicrobial susceptibility testing exhibited that 91.4%, 79.3%, 79.3%, 74.1%, and 58.6% of the isolates were sensitive to gentamicin, amoxicillin-clavulanic acid, ciprofloxacin, cephalexin, and sulfamethoxazole- trimethoprim, respectively. On the other hand, 91.4% and 70.7% isolates were resistant to cefotaxime and doxycycline, respectively. Cultivation on CRA revealed that 46.6% of isolates were biofilm producers. The molecular detection of resistance and virulence genes declared that all isolates harbored bla TEM , sul 1, tet A, qnr S, bla CTX-M , and fim H with a percentage of 100%, pap C (40%), and hly A (10%). Fim H was the most prevalent biofilm-associated gene. Conclusion: The present study highlights the high prevalence of multi-drug- resistant E. coli associated with bovine endometritis. The detection of the fim H gene is circumstantial evidenced that this gene has a crucial role in biofilm formation in intrauterine pathogenic E. coli .

Effect of aromatic oils on the expression of some virulence-associated and antimicrobial resistance genes of Escherichia coli isolated from broilers

“…In the present study, 60.7% of E. coli isolates were phenotypically positive biofilm formers on YESCA/CR agar. These results are higher than those of Amer et al [ 24 ], 35.7%, and Raheel et al [ 30 ], 46.6%, and lower than that obtained by Wang et al [ 31 ], 81.64%.…”

In-Depth Analysis of an Obligate Anaerobe Paraclostridium bifermentans Isolated from Uterus of Bubalus bubalis

“…The biofilm form by pathogenic bacteria helps to sequester antimicrobial agents and makes it difficult to clear the infections. P. melaninogenica , A. pyogenes , and E. coli have all been reported to infect uterine linings in cattle, and have also been found to produce biofilm in uterine linings, suggesting that biofilm formation could be a major cause of persistent uterine infections [ 44 , 45 ].…”

Differential efficacies of marigold leaves and turmeric paste on the healing of the incised wound in sheep

“…Then, the inoculated samples were incubated overnight to grow bacterial colonies at 37°C [ 17 ]. Gram stain protocol was conducted to know the staining and morphological characteristics of specific bacteria [ 18 ].…”