Biofuel cell based on direct bioelectrocatalysis

Ramanavičius, Arūnas; Kaušaitė, Asta

doi:10.1016/j.bios.2004.08.032

Cited by 191 publications

(98 citation statements)

References 28 publications

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“…These membrane-bound quinohaemoproteins have been studied for alcohol biosensing and alcohol-based EFCs [86,87]. Although PQQ-ALDH is a quinohaemoprotein and it has been used for studying the effect of orientation of the direct bioelectrocatalysis with multi-subunit proteins [88], PQQ-ADH is the more common enzyme studied electrochemically.…”

mentioning

confidence: 99%

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality

Milton

Minteer

2017

J. R. Soc. Interface.

166

148

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Enzymatic bioelectrocatalysis is being increasingly exploited to better understand oxidoreductase enzymes, to develop minimalistic yet specific biosensor platforms, and to develop alternative energy conversion devices and bioelectrosynthetic devices for the production of energy and/or important chemical commodities. In some cases, these enzymes are able to electronically communicate with an appropriately designed electrode surface without the requirement of an electron mediator to shuttle electrons between the enzyme and electrode. This phenomenon has been termed direct electron transfer or direct bioelectrocatalysis. While many thorough studies have extensively investigated this fascinating feat, it is sometimes difficult to differentiate desirable enzymatic bioelectrocatalysis from electrocatalysis deriving from inactivated enzyme that may have also released its catalytic cofactor. This article will review direct bioelectrocatalysis of several oxidoreductases, with an emphasis on experiments that provide support for direct bioelectrocatalysis versus denatured enzyme or dissociated cofactor. Finally, this review will conclude with a series of proposed control experiments that could be adopted to discern successful direct electronic communication of an enzyme from its denatured counterpart.

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mentioning

confidence: 99%

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality

Milton

Minteer

2017

J. R. Soc. Interface.

166

148

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“…The application of soluble redox mediators and/or detection of reaction substrates/products were applied in each case. However, direct (label-free) detection of analytes by bioelectronic devices [10] is the most promising and challenging opportunity in biosensorics. One of the most promising labelfree detection methods in biosensorics is based on detection of surface plasmon resonance since this method allows one to monitor binding events in real time without labeling.…”

mentioning

confidence: 99%

Surface plasmon resonance label‐free monitoring of antibody antigen interactions in real time

Kaušaitė

Dijk²,

Castrop³

et al. 2007

Biochem Molecular Bio Educ

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Detection of biologically active compounds is one of the most important topics in molecular biology and biochemistry. One of the most promising detection methods is based on the application of surface plasmon resonance for label-free detection of biologically active compounds. This method allows one to monitor binding events in real time without labeling. The system can therefore be used to determine both affinity and rate constants for interactions between various types of molecules. Here, we describe the application of a surface plasmon resonance biosensor for label-free investigation of the interaction between an immobilized antigen bovine serum albumin (BSA) and antibody rabbit anti-cow albumin IgG1 (anti-BSA). The formation of a self-assembled monolayer (SAM) over a gold surface is introduced into this laboratory training protocol as an effective immobilization method, which is very promising in biosensing systems based on detection of affinity interactions. In the next step, covalent attachment via artificially formed amide bonds is applied for the immobilization of proteins on the formed SAM surface. These experiments provide suitable experience for postgraduate students to help them understand immobilization of biologically active materials via SAMs, fundamentals of surface plasmon resonance biosensor applications, and determination of non-covalent biomolecular interactions. The experiment is designed for master and/or Ph.D. students. In some particular cases, this protocol might be adoptable for bachelor students that already have completed an extended biochemistry program that included a background in immunology.

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“…물리적 흡착만으로 제조된 촉매(GOx/PEI/CNT)는 Hyun 등에 의하 여 XPS, TEM, Electrophoretic light scattering spectrophotometer (Zeta-potential)으로 검증되었으며, 안정적으로 최대 담지가능한 GOx의 양은 CNT 2 mg당 2 mg으로 알려져 있다 [10]. 가교제 투입 효과를 확인하기 위하여 동일량의 GOx(CNT 2 mg [11,12]. …”

Section: 결과 및 토론unclassified

A Study on Performance Improvement of Glucose Sensor Adopting a Catalyst Using New Cross Liker

Chung¹,

Kwon²

2015

Korean Chemical Engineering Research

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− In this study, we synthesized a new biocatalyst consisting of glucose oxidase (GOx), polyethyleneimine (PEI) and carbon nanotube (CNT) with addition of terephthalaldehyde (TPA) (TPA/GOx/PEI/CNT) for fabrication of glucose sensor that shows improved sensing ability and stability compared with that using other biocatalysts. Main bonding of the new TPA/GOx/PEI/CNT catalyst is formed by Aldol condensation reaction of functional end groups between GOx/ PEI and TPA. Such formed bonding structure promotes oxidation reaction of glucose. Catalytic activity of TPA/GOx/PEI/CNT is evaluated quantitatively by electrochemical measurements. As a result of that, large sensitivity value of 41 µAcm -2 mM -1 is gained. Regarding biosensor stability of TPA/GOx/PEI/CNT catalyst, covalent bonding formed between GOx/PEI and TPA prevents GOx molecules from becoming leaching-out and contributes improvement in biosensor stability. With estimation of the biosensor stability, it is found that the TPA/GOx/PEI/CNT catalyst keeps 94.6% of its initial activity even after three weeks.

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Biofuel cell based on direct bioelectrocatalysis

Cited by 191 publications

References 28 publications

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality

Direct enzymatic bioelectrocatalysis: differentiating between myth and reality

Surface plasmon resonance label‐free monitoring of antibody antigen interactions in real time

A Study on Performance Improvement of Glucose Sensor Adopting a Catalyst Using New Cross Liker

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