Biofunctional Protein Fraction From Red Algae (Rhodophyta) Eucheuma Spinosum as an Antibacterial and Anticancer Drug Agent

Abstract

Protein fractions were isolated from red algae Eucheuma spinosum, this alga was derived from coast Takalar, Punaga Village, District Mangarabombang, Takalar. Protein fractions were macerated with Tris-HCl buffer. Ammonium sulfate was used to fractionation of proteins while determinations of protein content used Lowry method. The protein fractions were tested their activity against pathogenic bacteria. The fractions with the highest activity were combined with chloramphenicol and then was tested their activities back. The last test for protein fractions was screening toxicity; the method screening used Brine Shrimp Lethality Test (BSLT) method. The highest protein concentration on the crude extract was 96.76 mg/ml and the highest protein fraction concentration on a fraction of 20-40% (F2) was 88.42 mg/ml. The strongest protein fraction activity on a fraction 0-20% (F1) with inhibition zone is 9.09 mm to Escherichia coli bacteria and 9.56 mm to Staphylococcus aureus bacteria because the protein fraction F1 is highest so that it combined with chloramphenicol. All of a combination antibacterial activity was show an antagonistic effect against E. coli and S. aureus but did not for a combination of Protein Fraction F1: Chloramphenicol (95.35µg: 7.5µg), they shown additive effect (indifference) to S. aureus. The Protein fraction F1 from the Eucheuma spinosum algae was toxic to the Artemia salina Leach larvae; the value of LC50 was 7.252μg/mL. This fraction may be a potential anticancer drug agent, to prove it needs further research.

The Lebanese Red Algae Jania rubens: Promising Biomolecules against Colon Cancer Cells

“…Indeed, previous studies have shown that algal proteins possess significant biological potential as anti-inflammatory, antibacterial, and antioxidant compounds [ 27 , 28 ]. Moreover, the proteins extracted from red algae also have a potential antitumor effect [ 29 , 30 ]. Phycocyanin, a phycobiliprotein present in red algae, possesses an effective anticancer effect against MCF-7 breast cancer cells [ 31 ], HT29 colorectal carcinoma cells [ 32 ], A549 lung adenocarcinoma cells [ 33 ] and others.…”

Potential of Red Algae Eucheuma spinosum as Antibacterial to Pseudomonas aeruginosa

“…1 E. spinosum belongs to the Rhodophyceae group which is capable of producing carrageenan in large quantities. 2 This type of algae is used for traditional medicine because it does not cause side effects on health. 3 The ability of red algae E. spinosum to produce bioactive secondary metabolites are very likely due to extreme environmental conditions such as high salinity or used to defend itself from predators.…”