Biofunctionalized nanodot zirconia-based efficient biosensing platform for noninvasive oral cancer detection

Kumar, Suveen; Chauhan, Dipti; Renugopalakrishnan, V.; Malhotra, Bansi D.

doi:10.1557/mrc.2020.75

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…(5) Immunohistochemistry . Dewaxing Paraffin sections of 4 thickness were sequentially placed in 100% xylene (a) and 100% xylene (b) at 37°C for 15 min each The slices were sequentially placed in 100% ethanol-100% ethanol-95% ethanol-80% ethanol-70% ethanol-plus % ethanol for 2 minutes each The slices were soaked in double-distilled water for 2 minutes and repeated 3 times [ 15 ] (ii) Antigen Retrieval After dewaxing, the samples were placed in 3% H 2 O, treated at room temperature for 15 minutes, and then soaked in double-distilled water for 2 minutes, repeating 3 times Preheat the 0.1 m citric acid solution in the microwave for 3 minutes, quickly put the slices into the sodium citrate solution, and continue to boil for 15 to 20 minutes (iii) Serum Blocking The samples were cooled for 30 minutes and then soaked in double-distilled water for 2 minutes, repeating 3 times After the double-distilled water on the tissue evaporated, immediately add serum-containing peroxidase blocking agent to block, and incubate at 37°C for 30 minutes (iv) Antibody Incubation Incubate with primary antibody, incubate at 37°C for 30 minutes, discard the blocking solution, add TNF- α or IL-6 antibody, and incubate at 4°C overnight After incubation with the secondary antibody, after recovering the primary antibody, the samples were washed three times with PBS for 5 minutes each time. After washing, biotinylated rabbit anti-goat secondary antibody was added and incubated at room temperature for 15 minutes (v) Color Rendering …”

Section: Methodsmentioning

confidence: 99%

Clinical Analysis of Echocardiography and Serum IL-6 and TNF-α Changes in Pregnant Women with Hypertension

Liu

Hou

et al. 2022

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In order to explore the changes and clinical significance of serum TNF-α and IL-6 and ET levels in the pathogenesis of hypertensive disorders of pregnancy (HDIP), echocardiography, and serum IL-6 and TNF-α changes in pregnant women with a hypertensive disorder, a clinical analysis method was proposed. A retrospective analysis of 59 pregnant women who visited the obstetrics department of a provincial hospital was divided into 2 groups. The normal control group consisted of 32 normal, uncomplicated pregnant women; the preeclampsia group included 27 patients with systolic blood pressure > 140 mmHg and/or diastolic blood pressure > 90 mmHg who developed proteinuria after 20 weeks of gestation. The levels of TNF-α and IL-6 in serum of normal pregnant women and pregnant women with preeclampsia were detected by enzyme-linked immunosorbent assay (ELISA). The results showed that compared with normal pregnant women, the serum levels of TNF-α and IL-6 in the early pregnant women of Zizhi were significantly increased, and the trend of increased TNF-α and IL-6 levels was related to the severity of complications. With the mean pulmonary artery pressure > 50 mmHg , the serum TNF-α level of pregnant women was significantly higher than that of pregnant women with mean pulmonary artery pressure < 50 mmHg . The analysis found that the serum levels of TNF-α and IL-6 in patients with hypoxic gestational hypertension were significantly increased, and the results of lung tissue immunohistochemistry also showed that serum TNF-α and IL-6 levels in patients with hypoxic gestational hypertension were significantly increased. And serum TNF-α and IL-6 levels were positively correlated with right ventricular systolic blood pressure (RVSP). Conclusion. This study revealed that the elevated levels of serum TNF-α and IL-6 are closely related to the pathophysiological process of gestational hypertension. Serum levels of TNF-α and IL-6 and ET were significantly increased, and the changes of serum TNF-α and IL-6 and ET levels had important clinical value for closely monitoring the severity of the disease and the development of the disease.

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Section: Methodsmentioning

confidence: 99%

Clinical Analysis of Echocardiography and Serum IL-6 and TNF-α Changes in Pregnant Women with Hypertension

Liu

Hou

et al. 2022

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“…Clark and Lyon et al presented the concept of enzyme biosensors utilizing enzyme electrodes for the first time in 1962 (Clark et al, 2021). Horseradish, polyphenol oxidase, tyrosinase, lactate oxidase, peroxidase, alcohol dehydrogenase, cytochrome, glucose oxidase, and glutamate dehydrogenase are all often utilized in the construction of enzymatic biosensors (Kumar et al, 2020).…”

Section: Electrochemical Enzyme Biosensing Of Oral Diseasesmentioning

confidence: 99%

Advances in electrochemical biosensors for the detection of common oral diseases

Han

Liu

Guan

et al. 2023

Preprint

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With the advancement of society and people’s knowledge of oral health, the prevention of oral illnesses such as dental caries, periodontal disease, oral cancer, oral mucosal disease, salivary gland disease, and so on has become the primary focus of current health status and treatment. Although treatment for these diseases has focused on removing lesions or inhibiting progression, early detection has always been the focus of clinical research. And the non-invasive diagnosis is now the primary goal of clinical diagnosis. Meanwhile, the electrochemical approach can satisfy this need while also achieving a speedy and reliable diagnosis, giving patients a more comfortable option. And noninvasive detection of specific diseases can be achieved by detecting markers carried in body fluids. Therefore, in the context of disease, pathophysiological progress and electrochemical-assisted diagnosis form a close relationship, providing a basis for the detection of oral disease related indicators. As a result, this review comprehensively summarizes the detection and diagnosis of common oral diseases using electrochemical sensing technology and provides important insights for the development of electrochemical biosensors for the early detection of common oral diseases.

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“…Different NPs (ZrO 2 , nHfO 2 , and TiO 2 ) have been electrophoretically deposited onto APTES treated ITO coated glass electrode followed by conjugation with mAb‐anti‐CYFRA‐21‐1 and BSA as the blocking agent. (Kumar et al, 2015, 2020; Kumar, Kumar, et al, 2016; Kumar, Kumar, & Sharma, 2018). The measurements were performed in PBS buffer at pH 7 containing [Fe(CN) 6 ] 3−/4− as the electrolyte species.…”

Section: Nano‐biosensors: a Step Towards Advanced Detection Platformsmentioning

confidence: 99%

“…Different NPs (ZrO 2 , nHfO 2 , and TiO 2 ) have been electrophoretically deposited onto APTES treated ITO coated glass electrode followed by conjugation with mAb-anti-CYFRA-21-1 and BSA as the blocking agent. (Kumar et al, 2015(Kumar et al, , 2020 F I G U R E 1 3 (a) Amplified signal generation via nano-ELISA method for detection of oral cancer-specific biomarker and (b) graphical representation of nanosensor-based microfluidic approach for sensitive detection of oral cancer biomarker Kumar, Kumar, & Sharma, 2018). The measurements were performed in PBS buffer at pH 7 containing [Fe(CN) 6 ] 3À/4À as the electrolyte species.…”

Section: Electrochemical Nano-biosensormentioning

confidence: 99%

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

Chakraborty

Ghosh

Kumar

et al. 2022

WIREs Nanomed Nanobiotechnol

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Globally, oral cancer kills an estimated 150,000 individuals per year, with 300,000 new cases being diagnosed annually. The high incidence rate of oral cancer among the South-Asian and American populations is majorly due to overuse of tobacco, alcohol, and poor dental hygiene. Additionally, socioeconomic issues and lack of general awareness delay the primary screening of the disease. The availability of early screening techniques for oral cancer can help in carving out a niche for accurate disease prognosis and also its prevention. However, conventional diagnostic approaches and therapeutics are still far from optimal. Thus, enhancing the analytical performance of diagnostic platforms in terms of specificity and precision can help in understanding the disease progression paradigm. Fabrication of efficient nanoprobes that are sensitive, noninvasive, cost-effective, and less labor-intensive can reduce the global cancer burden. Recent advances in optical, electrochemical, and spectroscopy-based nano biosensors that employ noble and superparamagnetic nanoparticles, have been proven to be extremely efficient. Further, these sensitive nanoprobes can also be employed for predicting disease relapse after chemotherapy, when the majority of the biomarker load is eliminated. Herein, we provide the readers with a brief summary of conventional and new-age oral cancer detection techniques. A comprehensive understanding of the inherent challenges associated with conventional oral cancer detection techniques is discussed. We also elaborate on how nanoparticles have shown tremendous promise and effectiveness in radically transforming the approach toward oral cancer detection.

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Biofunctionalized nanodot zirconia-based efficient biosensing platform for noninvasive oral cancer detection

Cited by 11 publications

References 31 publications

Clinical Analysis of Echocardiography and Serum IL-6 and TNF-α Changes in Pregnant Women with Hypertension

Clinical Analysis of Echocardiography and Serum IL-6 and TNF-α Changes in Pregnant Women with Hypertension

Advances in electrochemical biosensors for the detection of common oral diseases

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

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