Biogeographical Variation and Population Genetic Structure of<i>Sporisorium scitamineum</i>in Mainland China: Insights from ISSR and SP-SRAP Markers

Xu, Liping; Lu, Yong-Zai; You, Qi; Liu, Xiaolan; Grisham, M. P.; Pan, Yong‐Bao; Que, Youxiong

doi:10.1155/2014/296020

Cited by 12 publications

(21 citation statements)

References 41 publications

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“…Molecular detection of the smut pathogen in sugarcane has become possible by using polymerase chain reaction (PCR) to amplify the bE mating-type gene of S. scitamineum (Byther and Steiner, 1974;Albert and Schenck, 1996;Schenck, 1998;Singh et al, 2004;Su et al, 2013). Random-amplified polymorphic DNA, amplified fragment length polymorphism, sequence-related amplified polymorphism, inter-simple sequence repeat, and internal transcribed spacer (ITS) sequence analysis have been used to evaluate intraspecies diversity within S. scitamineum isolates (Victoria et al, 1997;Bakkeren et al, 2000;Braithwaite et al, 2004;Stoll et al, 2003Stoll et al, , 2005Xu et al, 2004Xu et al, , 2014Singh et al, 2005;Baraket et al, 2009;Que et al, 2012Que et al, , 2014a. Therefore, molecular methods can be applied for phylogeny analysis because the phylogeny of S. scitamineum is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

et al. 2015

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ABSTRACT. Sugarcane smut caused by the fungus Sporisorium scitamineum is a worldwide disease and also one of the most prevalent diseases in sugarcane production in mainland China. To study molecular variation in S. scitamineum, 23 S. scitamineum isolates from the 6 primary sugarcane production areas in mainland, China (Guangxi, Yunnan, Guangdong, Hainan, Fujian, and Jiangxi Provinces), were assessed using internal transcribed spacer (ITS) methods. The results of ITS sequence analysis showed that the organisms can be defined at the genus level, including Ustilago and Sporisorium, and can also differentiate between closely related species. This method was not suitable for phylogenetic relationship analysis of different S. scitamineum isolates and could not provide support regarding their race ascription at the molecular level. The results of the present study will be useful for studies examining the molecular diversity of S. scita-7895 Molecular variation of Sporisorium scitamineum ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7894-7909 (2015) mineum and for establishing a genetic foundation for their pathogenicity differentiation and new race detection. In addition, our results can provide useful information for the pathogen selection principle in sugarcane smut resistance breeding and variety distribution.

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Section: Introductionmentioning

confidence: 99%

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

et al. 2015

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“…In addition, this report also showed that the American and African populations all belonged to a single lineage which was also found among some Asian populations. Previous studies (Que et al, 2012;Shen et al, 2012a;Xu et al, 2014) on genetic diversity of S. scitamineum collected from China showed that the molecular diversity of S. scitamineum was associated with its geographical origin. Shen et al (2012a) also suggested that majority of S. scitamineum in sugarcane-producing regions of Southern China may belong to or are genetically similar to race 3.…”

Section: Introductionmentioning

confidence: 98%

“…The disease has caused serious problems in sugarcane plantation and sugar production, especially in the last 15 years due to the susceptibility of the sugarcane cultivar ROC22 which occupies more than 50% of the total sugarcane planting area in China. The average of smut infection rate in ROC22 is over 8% in plant cane and 15% in ratoon cane, respectively (Shen et al, 2013;Xu et al, 2014). The disease has now caused the most serious economic loss of sugarcane in China.…”

Section: Introductionmentioning

confidence: 99%

“…The investigation of the genetic diversity of S. scitamineum through the use of DNA molecular fingerprinting techniques is extremely important for a better understanding of the structure of pathogen populations and the interactions among host and pathogen and the environment and as a basis for developing smut-resistant sugarcane varieties. During the past ten years, several studies on genetic diversity of S. scitamineum were conducted in Australia (Braithwaite et al, 2004), France (Raboin et al, 2007) and China (Que et al, 2012;Shen et al, 2012a;Xu et al, 2014). Raboin et al (2007) analyzed a collection of S. scitamineum populations from 15 sugarcane-producing countries for polymorphisms at 17 microsatellite loci.…”

Section: Introductionmentioning

confidence: 99%

“…Teliospore and mating-type haploid sporidia have their respective genetic background. In the previous studies (Que et al, 2012;Xu et al, 2014), DNA was extracted from teliospores of S. scitamineum on the basis of genetic evolution of S. scitamineum from Mainland China. Although, DNA was extracted from mating-type haploid sporidia in the report of Shen et al (2012a), only 35 samples collected from 6 sugarcane-producing areas in three provinces of China were analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of genetic diversity and structure of Sporisorium scitamineum from China using inter-simple sequence repeat (ISSR) markers

Xu¹,

Deng²,

Shen³

et al. 2017

Afr. J. Biotechnol.

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In this study, the genetic diversity and structure of 90 monosporidia mating-type isolates of Sporisorium scitamineum collected from China were comprehensively evaluated based on the DNA extracted from mating-type haploid sporidias using inter-simple sequence repeat (ISSR) polymorphism molecular marker technique. Fifteen selected ISSR primers produced 162 amplified DNA fragments, of which 115 (71.0%) were polymorphic. Polymorphic information content (PIC) values ranged from 0.12 to 0.92 with an average of 0.82, indicating that a moderate degree of genetic diversity exists among these 90 monosporidia mating-type isolates of S. scitamineum collected from China. Genetic differentiation coefficient (G st ) was calculated to be 0.320, indicating that the total genetic variation between populations was 32%, while 68% of the total genetic variation was from within populations. Meanwhile, gene flow (N m ) was 1.063, indicating lower rate of gene flow between populations. Based on the ISSR marker data set, the results of clustering by the unweighted pair group method of arithmetic average and principal component analysis were similar and could divide these 90 S. scitamineum isolates into two main groups. The isolates collected from the same province tend to cluster in the same main groups. This study shows that the genetic differentiation of these 90 monosporidia mating-type isolates of S. scitamineum was highly correlated with the ecological environment of collection sites, and the heterogeneity of ecological environment was the main driving force for the differentiation of S. scitamineum.

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Field assessment and combining ability analysis for smut resistance in sugarcane during continuous ratoon planting in China

Xu,

Wu,

Gillani

et al. 2024

Crop Science

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Sugarcane smut, caused by Sporisorium scitamineum, poses a significant threat to sugarcane production in China, resulting in substantial economic losses. This study aims to understand the resistance of sugarcane genotypes to smut and the relationship between smut incidence and yield loss during ratoon cropping. We evaluated the smut resistance of 169 sugarcane genotypes. We assessed the combining ability of nine female and six male parent plants in field trials conducted from 2019 to 2022 in Fusui or Longzhou, China. Our findings reveal increased smut incidence with each successive ratoon cycle, particularly after the second ratoon. Smut infection during the ratoon cycle reduced sugarcane yield by decreasing the number of millable stalks. Variance analysis indicated that genotype, cropping cycle, and location significantly influenced disease incidence. Based on resistance levels, the genotypes were categorized into five groups: 10 highly resistant, 37 resistant, 38 moderately susceptible, 42 susceptible, and 42 highly susceptible. Analysis of general combining ability (GCA) and specific combining ability (SCA) demonstrated that crossing sugarcane parents with smut resistance effectively produced resistant hybrid offspring. Additionally, the maternal inheritance effect was more pronounced than the paternal inheritance effect, indicating that using a resistant genotype as the maternal parent is more advantageous for enhancing smut resistance. These results provide valuable insights into sugarcane smut resistance, aiding in selecting parental lines and breeding new resistant varieties.

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Biogeographical Variation and Population Genetic Structure ofSporisorium scitamineumin Mainland China: Insights from ISSR and SP-SRAP Markers

Cited by 12 publications

References 41 publications

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

Molecular variation of Sporisorium scitamineum in Mainland China revealed by internal transcribed spacers

Assessment of genetic diversity and structure of Sporisorium scitamineum from China using inter-simple sequence repeat (ISSR) markers

Field assessment and combining ability analysis for smut resistance in sugarcane during continuous ratoon planting in China

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