Biogeography, Speciation and Taxonomy within the genus Bactrocera Macquart with application to the Bactrocera dorsalis (Hendel) complex of fruit flies (Diptera: Tephritidae: Dacinae)

Drew, R. A. I.; Hancock, D. L.

doi:10.11646/zootaxa.5190.3.2

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…But Drew and Romig proposed the withdrawal of this result (Drew and Romig, 2016). Further evidence based on the male aedeagus showed that B. phillipinensis and B. invadens differed from B. dorsalis (Drew et al, 2022), confirming this withdrawal. Our results show that B. dorsalis is more closely related to B. thailandica than B. phillipinensis and B. invadens (Figure 2; Supplementary Figures S9-S11).…”

Section: Phylogeny Of the Fruit Fliesmentioning

confidence: 55%

Phylogenomic analysis and molecular identification of true fruit flies

He,

Wang,

et al. 2024

Front. Genet.

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The family Tephritidae in the order Diptera, known as true fruit flies, are agriculturally important insect pests. However, the phylogenetic relationships of true fruit flies, remain controversial. Moreover, rapid identification of important invasive true fruit flies is essential for plant quarantine but is still challenging. To this end, we sequenced the genome of 16 true fruit fly species at coverage of 47–228×. Together with the previously reported genomes of nine species, we reconstructed phylogenetic trees of the Tephritidae using benchmarking universal single-copy ortholog (BUSCO), ultraconserved element (UCE) and anchored hybrid enrichment (AHE) gene sets, respectively. The resulting trees of 50% taxon-occupancy dataset for each marker type were generally congruent at 88% nodes for both concatenation and coalescent analyses. At the subfamily level, both Dacinae and Trypetinae are monophyletic. At the species level, Bactrocera dorsalis is more closely related to Bactrocera latifrons than Bactrocera tryoni. This is inconsistent with previous conclusions based on mitochondrial genes but consistent with recent studies based on nuclear data. By analyzing these genome data, we screened ten pairs of species-specific primers for molecular identification of ten invasive fruit flies, which PCR validated. In summary, our work provides draft genome data of 16 true fruit fly species, addressing the long-standing taxonomic controversies and providing species-specific primers for molecular identification of invasive fruit flies.

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Section: Phylogeny Of the Fruit Fliesmentioning

confidence: 55%

Phylogenomic analysis and molecular identification of true fruit flies

He,

Wang,

et al. 2024

Front. Genet.

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“…Lalat buah (Bactrocera spp) berpotensi dalam menurunkan hasil produksi usaha tani (Drew & Hancock, 2022). Serangan lalat buah mengakibatkan kerugian baik dari sisi kualitas maupun kuantitas hasil produksi dengan intensitas serangan mencapai 100% (Kaurow et al, 2015) sehingga dapat menyebabkan gagal panen.…”

Section: Pendahuluanunclassified

“…Selain itu, Tanaman cabai merupakan tanaman inang utama dari spesies Bactrocera dorsalis (Al Rahmat et al, 2021). Sedangkan, Bactrocera carambolae menyerang tanaman cabai walaupun bukan tanaman inang utama, tetapi spesies lalat ini memiliki sifat polifag atau dapat hidup lebih dari satu tanaman inang (Drew & Hancock, 2022). Jenis lalat buah yang memiliki sifat polifag pada fase bertelur akan meletakan telurnya ke dalam buah.…”

Section: Identifikasi Jenis Lalat Buahunclassified

Uji Efektivitas Tanaman Refugia dalam Menurunkan Intensitas Serangan Lalat Buah pada Cabai Rawit

Kurniawan,

Setiawan

2024

J. Ilm. Membangun Desa Pertanian

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Fruit flies (Bactrocera spp.) are pests that attack cayenne peppers, with attacks reaching 100%. Generally, farmers use synthetic insecticides, but in the long term this can disrupt the balance of the ecosystem and trigger pest outbreaks and resistance—integrated pest control by planting refugia as a micro-habitat for natural enemies. Marigolds and zinnias were chosen as refugia plants. The aim of the research was to determine the types of fruit flies that attack chili plants, the symptoms of fruit fly attacks on chili fruit, the effectiveness of marigold and zinnia plants in reducing the intensity of chili plant attacks, and external factors that influence the efficacy of refugia marigold and zinnia plants. The research was conducted in July – October 2023 in Jlarem Village, Gladaksari District, Boyolali Regency, using a Randomized Block Design (RAK) with four treatments, namely control, marigold, zinnia, and a combination of both, using six replications so that there were 24 experimental units with ten plants in each experimental unit. This research used a hygrometer thermometer (HTC2), camera, net, and binocular microscope. Observation variables include the type of fruit fly, attack symptoms, attack intensity, and external factors. The types of fruit flies found in the study area were Bactrocera dorsalis, Bactrocera umbrosa, and Bactrocera carambolae. Symptoms that arise due to fruit fly attacks on chili fruit are black spots on the skin of the fruit, which become more expansive over time, causing the fruit to rot and fall prematurely. Refugia planting effectively reduced the intensity of fruit fly attacks, with the lowest attack intensity in the combination treatment of marigold and zinnia at 22.16%. Several external factors influence the effectiveness of refugia in controlling fruit flies, namely environmental temperature and humidity and the types of plants growing around the cultivated land.

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“…Bactrocera philippinensis , B. papaya , and B. invadens were previously considered junior synonyms of B. dorsalis ( Schutze et al., 2015 ; Zeng et al., 2019 ). Nonetheless, Drew and Hancock (2022) have delineated them as distinct entities from B. dorsalis based on differences in the shapes and dimensions of the glans and preglans appendix. The maggot of B. dorsalis poses the most significant threat, with adult females utilizing their spiny ovipositors to penetrate the fruit skin and laying eggs beneath the rind.…”

Section: Introductionmentioning

confidence: 99%

A simple PCR-based quick detection of the economically important oriental fruit fly, Bactrocera dorsalis (Hendel) from India

Arya,

Narayana,

Sinha

et al. 2024

Front. Plant Sci.

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The oriental fruit fly, Bactrocera dorsalis (Hendel), is a significant economic and quarantine pest due to its polyphagous nature. The accurate identification of B. dorsalis is challenging at the egg, maggot, and pupal stages, due to lack of distinct morphological characters and its similarity to other fruit flies. Adult identification requires specialized taxonomist. Existing identification methods are laborious, time consuming, and expensive. Rapid and precise identification is crucial for timely management. By analyzing the variations in the mitochondrial cytochrome oxidase-1 gene sequence (Insect barcoding gene), we developed a species-specific primer (SSP), DorFP1/DorRP1, for accurate identification of B. dorsalis. The optimal annealing temperature for the SSP was determined to be 66°C, with no cross-amplification or primer-dimer formation observed. The SSP was validated with B. dorsalis specimens from various locations in northern and eastern India and tested for cross-specificity with six other economically significant fruit fly species in India. The primer specificity was further confirmed by the analysis of critical threshold (Ct) value from a qPCR assay. Sensitivity analysis showed the primer could detect template DNA concentrations as low as 1 pg/µl, though sensitivity decreased at lower concentrations. Sequencing of the SSP-amplified product revealed over >99% similarity with existing B. dorsalis sequences in the NCBI GenBank. The developed SSP reliably identifies B. dorsalis across all developmental stages and sexes. This assay is expected to significantly impact pest identification, phytosanitary measures, and eradication programs for B. dorsalis.

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Biogeography, Speciation and Taxonomy within the genus Bactrocera Macquart with application to the Bactrocera dorsalis (Hendel) complex of fruit flies (Diptera: Tephritidae: Dacinae)

Cited by 11 publications

References 25 publications

Phylogenomic analysis and molecular identification of true fruit flies

Phylogenomic analysis and molecular identification of true fruit flies

Uji Efektivitas Tanaman Refugia dalam Menurunkan Intensitas Serangan Lalat Buah pada Cabai Rawit

A simple PCR-based quick detection of the economically important oriental fruit fly, Bactrocera dorsalis (Hendel) from India

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