Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates

Damayanti, Astrilia; Sarto, Sarto; Syamsiah, Siti; Sediawan, Wahyudi Budi

doi:10.1063/1.4938299

Cited by 3 publications

(6 citation statements)

References 16 publications

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“…Mixed culture was obtained from biodigester of tofu waste, cow dung, and fruit waste in Indonesia. The characteristics and pretreatment of mixed culture were similar to the previous experiment (Damayanti et al, 2015).…”

Section: Mixed Culturesupporting

confidence: 73%

“…The initial concentrations of glucose (Merck, 97.5%) and DL-limonene (Merck, >95%) was 10,000 ppm and 60 ppm, respectively. The same compositions of the fermentation enrichment medium and nutrients were used on the previous experiment (Damayanti et al, 2015).…”

Section: Materials and Methods Substrate And Medium Compositionmentioning

confidence: 99%

“…The bead making in this study was similar to that of the previous experiment (Damayanti et al, 2015). Beads consisted of three types i.e no mixed culture of immobilization and co-immobilization beads, and at last, free mixed culture co-immobilization beads.…”

Section: Preparation Of Immobilization and Coimmobilization Beadsmentioning

confidence: 96%

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Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads

Damayanti

Sarto

Sediawan

2018

Reaktor

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Rotten oranges contain glucose and limonene, in which limonene is an inhibitor of microorganisms. Immobilization of mixed culture used the entrapment method is the easiest method of protecting the mixed culture from inhibitors. Entrapment method with extrusion drip is an efficient and effective technique to produce beads. This study aims to determine the adsorption rate of adsorbate (glucose and limonene) on the adsorbent surface (beads). Materials used in this study were glucose, DL-limonene, mixed culture, and beads. Three types of beads consisted of alginate - no mixed culture (A), alginate and activated carbon - no mixed culture (CA), alginate and activated carbon - free mixed culture (CB). Adsorption column consist of 30 ml nutrient, 15 mL substrate, and 5 mL beads. If the beads do not contain mixed culture, nutrients and substrate were replaced by aquadest. The reactor was done in a batch system at 37oC. The lowest order of beads ability to adsorb glucose were AG followed by CAG and finally CBG, whereas to limonene solution were AL followed by CBL and finally CAL. Lagergren model was used to determined kinetic bioadsorption on limonene and glucose. The adsorption rate value in the pseudo-second order (k2,ad) for the glucose solution was ranged between 0.025 to 0.087 min-1, while the D-limonene was in the range between 2.084 to 5.233 min-1. Adsorption of glucose and limonene on the surface of the three types of adsorbents was reached steady state at the 60th minute.Keywords: orange, limonene, immobilization, adsorption, Lagergren model.

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Section: Mixed Culturesupporting

confidence: 73%

Section: Materials and Methods Substrate And Medium Compositionmentioning

confidence: 99%

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Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads

Damayanti

Sarto

Sediawan

2018

Reaktor

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“…The composition of the enrichment medium and fermentation nutrients used were similar to previous experiment [33].…”

Section: Substrate and Composition Mediummentioning

confidence: 98%

“…The number of beads used was the same as the previous experiments [33]. The average weight of immobilized beads and coimmobilization for the three concentrations used in hydrogen production were 3 g and 3.…”

Section: Batch Hydrogen Productionmentioning

confidence: 99%

Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production

Damayanti¹,

Sarto²,

Sediawan³

et al. 2018

J. MECH. ENG. SCI.

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This paper presents an experimental investigation on using mixed culture for immobilization and co-immobilization for hydrogen production. The shape and diameter of the beads were investigated. Hydrogen was produced from 10 g.L 1 glucose in anaerobic batch using immobilized mixed culture with extrusion dripping method. The alginate concentrations as immobilization material were 1%, 2%, and 3%. The mixed culture had three different biodigester sources consisting of cow dung, tofu waste, and fruit waste. The pretreatment of each mixed culture was acidification and enrichment. Then the mixed culture were mixed with immobilization material and inserted into a syringe, then dropped into 0.1M CaCl 2 . Activated carbon was added to alginate (coimmobilization) with ratio 1:1. The results showed that bead using 1% and 2% alginate concentrations were a pear-shaped. The highest concentration of hydrogen (mol H 2 /mol glucose) was 0.029 for immobilized beads with 2% alginate concentration and the lowest hydrogen (molH 2 /mol glucose) was 0.009 for immobilized beads with 3% alginate concentration. Acetic acid was the most dominant.

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Biohydrogen from the Organic Fraction of Municipal Solid Waste

Salazar-Batres

Quijano

Moreno‐Andrade

2022

Clean Energy Production Technologies

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Biohydrogen production from rotten orange with immobilized mixed culture: Effect of immobilization media for various composition of substrates

Cited by 3 publications

References 16 publications

Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads

Kinetic Study of limonene and glucose adsorption on immobilization and coimmobilization beads

Performance analysis of immobilized and co-immobilized enriched-mixed culture for hydrogen production

Biohydrogen from the Organic Fraction of Municipal Solid Waste

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