Bioimaging with Micro/Nanoelectrode Systems

Matsue, Tomokazu

doi:10.2116/analsci.29.171

“…[5][6][7][8] They can be functionalized in manifold ways to comprise electrochemical sensors 9 to access the chemical microenvironment 10,11 and probe the interior of single living cells. Our approach is based on the use of bifunctional carbon nanoelectrodes.…”

Section: Introductionmentioning

confidence: 99%

The oxygen reduction reaction at the three-phase boundary: nanoelectrodes modified with Ag nanoclusters

Clausmeyer

¹

,

Botz

²

,

Öhl

³

et al. 2016

View full text Add to dashboard Cite

Silver nanoclusters are deposited on bifunctional Θ-shaped nanoelectrodes consisting of a carbon nanoelectrode combined with a hollow nanopipette. The Θ-nanoelectrodes are used as model systems to study interfacial mass transport in gas diffusion electrodes and in particular oxygen-depolarized cathodes (ODC) for the oxygen reduction reaction (ORR) in chlor-alkali electrolysers. By local delivery of O gas to the electroactive Ag nanoclusters through the adjacent nanopipette, enhanced currents for the ORR at the Ag nanoparticles are recorded which are not accountable when considering the low solubility and slow diffusion of O in highly alkaline media. Instead, local oversaturation of O leads to current enhancement at the Ag nanoclusters. Due to the intrinsic high mass transport rates at the nanometric electrodes accompanied by local delivery of reactants, the method generally allows to study electrochemical reactions at single nanoparticles beyond the limitations induced by slow diffusion and low reactant concentration. Kinetic and mechanistic information, for instance derived from Tafel slopes, can be obtained from kinetic regimes not accessible to standard techniques.

show abstract

“…Micrometer spatial resolution with electrochemical methods can be achieved by using scanning electrochemical microscopy (SECM) where microelectrodes are laterally translated in close vicinity to a substrate inside an electrolyte solution containing redox active species [32][33][34][35][36][37][38]. Typical SECM applications range from bio-imaging, surface reactivity mapping to the characterization of liquid-liquid interfaces [39][40][41][42]. Conventionally, SECM is operated in constant height mode requiring flat and non-tilted substrates.…”

Section: Introductionmentioning

confidence: 99%

Mapping the Antioxidant Activity of Apple Peels with Soft Probe Scanning Electrochemical Microscopy

Lin

¹

2018

Soft Probes for Bio-Electrochemical Imaging

View full text Add to dashboard Cite

We present a non-invasive electrochemical strategy for mapping the antioxidant (AO) activity of apple peels, which counterbalances oxidative stress caused by various external effectors. Soft carbon microelectrodes were used for soft probe scanning electrochemical microscopy (SECM) enabling the gentle and scratch-free contact mode scanning of rough and delicate apple peels in an electrolyte solution. The SECM feedback mode was applied using ferrocene methanol (FcMeOH) as redox mediator that gets electrochemically oxidized at the soft probe and diffuses towards the apple peel where it gets regenerated by certain AOs leading to a redox mediator recycling and increased current signal. The global AO activity in the apple peel including lenticels and regions with artificially degraded AOs were mapped using the soft microelectrodes. Finally, in an apple cross-section the higher and homogeneous AO concentration in the peel with a heterogeneously decaying AO gradient towards the apple inward was visualized, demonstrating the adequate micrometer resolution of the SECM probe and the possibility to get information of the interior AO activity of the apple.

show abstract

“…Micrometer spatial resolution with electrochemical methods can be achieved by using scanning electrochemical microscopy (SECM) where microelectrodes are laterally translated in close vicinity to a substrate inside an electrolyte solution containing redox active species [32][33][34][35][36][37][38]. Typical SECM applications range from bio-imaging, surface reactivity mapping to the characterization of liquid-liquid interfaces [39][40][41][42]. Conventionally, SECM is operated in constant height mode requiring flat and non-tilted substrates.…”

Section: Introductionmentioning

confidence: 99%

Mapping the antioxidant activity of apple peels with soft probe scanning electrochemical microscopy

Lin

¹

,

Lesch

²

,

Li

³

et al. 2017

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

We present a non-invasive electrochemical strategy for mapping the antioxidant (AO) activity of apple peels, which counterbalances oxidative stress caused by various external effectors. Soft carbon microelectrodes were used for soft probe scanning electrochemical microscopy (SECM) enabling the gentle and scratch-free contact mode scanning of rough and delicate apple peels in an electrolyte solution. The SECM feedback mode was applied using ferrocene methanol (FcMeOH) as redox mediator that gets electrochemically oxidized at the soft probe and diffuses towards the apple peel where it gets regenerated by certain AOs leading to a redox mediator recycling and increased current signal. The global AO activity in the apple peel including lenticels and regions with artificially degraded AOs were mapped using the soft microelectrodes. Finally, in an apple cross-section the higher and homogeneous AO concentration in the peel with a heterogeneously decaying AO gradient towards the apple inward was visualized, demonstrating the adequate micrometer resolution of the SECM probe and the possibility to get information of the interior AO activity of the apple.

show abstract

Bioimaging with Micro/Nanoelectrode Systems

Cited by 27 publications

References 44 publications

The oxygen reduction reaction at the three-phase boundary: nanoelectrodes modified with Ag nanoclusters

The oxygen reduction reaction at the three-phase boundary: nanoelectrodes modified with Ag nanoclusters

Mapping the Antioxidant Activity of Apple Peels with Soft Probe Scanning Electrochemical Microscopy

Mapping the antioxidant activity of apple peels with soft probe scanning electrochemical microscopy

Contact Info

Product

Resources

About