Bioimprinting of Polymers and Sol−Gel Phases. Selective Detection of Yeasts with Imprinted Polymers

and, F. L. Dickert; Hayden, Oliver

doi:10.1021/ac010642k

Cited by 240 publications

(169 citation statements)

References 27 publications

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“…As already shown in previous studies, different types of microorganisms can also be detected by molecular imprinted polymers [7,15,16]. Detection and quantification of whole yeast cells imprinted on films made of polyurethane and alkoxide through sol-gel technique was obtained by application of QCM [14].…”

Section: Introductionmentioning

confidence: 75%

“…Barreau et al [11] and Livage [12] showed that entrapment of parasitic protozoa in sol-gel does not affect cells' structure. Imprinting of whole cells in sol-gel was reported by Chia [13], and further developed by Dickert [14,15]. These authors manufactured a polyurethane surface imprinted with S. cerevisiae yeasts allowing a selective and highly sensitive enrichment of the microorganisms under flow conditions.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Physical Parameters on Bacterial Cell Adsorption onto Pre-Imprinted Sol-Gel Films

Starosvetsky¹,

Cohen²,

Cheruti³

et al. 2012

JBNB

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Organically modified silica (ORMOSILS) thin films produced by sol-gel method were imprinted with two bacterial strains as whole cells in order to develop an easy, fast and specific probe to detect and specifically identify these microorganisms when present in water samples. An important feature of the imprinting process was the molecular fingerprints left by these microorganisms alongside morphology, into imprinted film cavities. The films also showed high selectivity toward the imprinted template and were able to discriminate between two very close bacterial species (E. coli and S. typhimurium). In addition, several central physical parameters of the experimental water solution were examined (i.e., pH, ionic strength and the organic load exemplified by NaCl and TOC concentration, respectively). The method sensitivity to different bacterial concentrations was studied by confocal microscopy (CLSM) and quartz crystal microbalance (QCM) tools. Results showed that increased bacterial concentrations favor rapid adsorption onto imprinted sol-gel films with high affinity, while low pH, increased organic load and high ionic concentrations (i.e., seawater) interfere with bacteria re-adsorption, reducing detection capability. Under average drinking water chemical composition the method proved to be highly efficient.

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Effects of Physical Parameters on Bacterial Cell Adsorption onto Pre-Imprinted Sol-Gel Films

Starosvetsky¹,

Cohen²,

Cheruti³

et al. 2012

JBNB

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show abstract

“…Several approaches to the development of antibody-like (and therefore biomimetic) binders have also been described in recent years. These include bioimprinted sol-gel polymers for binding whole yeast cells (83), peptide-based binders (84,85,86) and "affibodies" -non-antibody binders derived from randomizable small protein scaffolds (e.g. staphylococcal protein A) (87,88).…”

Section: Biomimeticsmentioning

confidence: 99%

Methods for Whole Cell Detection of Microorganisms

Brehm-Stecher

2008

ACS Symposium Series

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Microbes are ubiquitous, and can be found occupying nearly every imaginable niche on Earth. These include organic and inorganic surfaces, interfacial boundaries and within macroscopically solid matrices, such as the pore space of rocks. Because phylogenetically divergent microbes may be visually indistinguishable, understanding the species distribution and ecological significance of environmental microbes requires diagnostic tools that extend beyond simple phenotypic description. Methods for microbial diagnostics can be divided into two broad categories: cellular and acellular. Acellular techniques, such as the polymerase chain reaction or certain immunoassay formats may be effective at detecting molecular, structural or biochemical targets associated with specific cell types, but this information is provided out of its "natural", and arguably most meaningful context -that of the individual microbial cell. In contrast, cellular methods have the potential to preserve an abundance of valuable information. Apart from molecular identity, this includes information regarding cell morphology and other physiological characteristics, cell number and distribution within a sample, and physical or spatial associations with other cell types. The aim of this chapter is to provide an overview of whole cell methods for microbial detection, including both existing approaches and those still in development. The tools described here are expected to find wide application for the detection of microbes on surfaces or within complex matrices across a number of parallel or allied fields, including environmental, food and clinical microbiologies.

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“…The major impetus in the research and development of such virus sensors is their practical features such as the ease of operation, simple and straightforward device fabrication, possibility to integrate synthetic or natural antibodies, rapid response, high selectivity and cross-sensitivity, portability, and miniaturization capability. It is due to these advantages that biosensors have not only been widely employed for virus recognition [14], but have also been applied for the detection of microorganism such as pathogenic bacteria [15,16] and yeast [17][18][19][20], living blood cells [21][22][23], and different diseases biomarkers [24,25].…”

Section: Introductionmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Bioimprinting of Polymers and Sol−Gel Phases. Selective Detection of Yeasts with Imprinted Polymers

Cited by 240 publications

References 27 publications

Effects of Physical Parameters on Bacterial Cell Adsorption onto Pre-Imprinted Sol-Gel Films

Effects of Physical Parameters on Bacterial Cell Adsorption onto Pre-Imprinted Sol-Gel Films

Methods for Whole Cell Detection of Microorganisms

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

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