Bioinformatics Analysis for the Antirheumatic Effects of Huang-Lian-Jie-Du-Tang from a Network Perspective

Fang, Haiyang; Wang, Yichuan; Yang, Tinghong; Ga, Yang; Zhang, Yi; Liu, Runhui; Zhang, Guoqing; Zhao, Jing

doi:10.1155/2013/245357

Cited by 24 publications

(19 citation statements)

References 56 publications

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“… Han et al (2017) showed that combination treatment with metformin, a classic antidiabetic drug, and Scutellariae Radix could improve obesity-induced insulin resistance and lipid metabolism by maintaining bile acid homeostasis through reducing the amount of harmful bacteria and altering the gut microbiota structure. From the perspective of holistic view of TCM, different herbs and multiple components in HLJDD could act on multiple targets and exert an enhancing synergistic effect ( Fang et al, 2013 ). Thus, the results based on the previous studies are partial and indirect.…”

Section: Introductionmentioning

confidence: 99%

Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Chen

Liao

et al. 2018

Front. Microbiol.

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Background: Huang-Lian-Jie-Du-Decoction (HLJDD), a prescription of traditional Chinese medicine, has been clinically used to treat diabetes for thousands of years and its mechanism was reported to be related to gut microbiota. However, no study has explored the effect of HLJDD on the gut microbiota in type 2 diabetes mellitus (T2DM) yet. Therefore, in this study, we investigated the modulation of gut microbiota induced by HLJDD treatment in T2DM in order to unveil the underlying mechanism.Methods: A combination of high-fat diet (HFD) and streptozotocin (STZ) was used to induce T2DM in rats. Bacterial communities in the fecal samples from the control group, the T2DM model group, and the HLJDD-treated T2DM group were analyzed by 16S gene sequencing, followed with a subset sample analyzed by shotgun sequencing.Results: The HLJDD treatment significantly ameliorated hyperglycemia and inflammation in T2DM rats. Additionally, our results indicated that HLJDD treatment could not only restore the gut dysbiosis in T2DM rats, which was proved by an increasing amount of short chain fatty acids (SCFAs)-producing and anti-inflammatory bacteria such as Parabacteroides, Blautia, and Akkermansia as well as a decreasing amount of conditioned pathogenic bacteria (e.g., Aerococcus, Staphylococcus, and Corynebacterium), but also modulate the dysregulated function of gut microbiome in T2DM rats, including an up-regulation in bile acid biosynthesis as well as a reduction in glycolysis/gluconeogenesis and nucleotide metabolism.Conclusion: HLJDD treatment could ameliorate hyperglycemia and restore the dysregulated microbiota structure and function to a normal condition mainly by increasing SCFAs-producing bacteria and reducing conditioned pathogenic bacteria in T2DM rats, which provides insights into the mechanism of HLJDD treatment for T2DM from the view of gut microbiota.

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Section: Introductionmentioning

confidence: 99%

Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Chen

Liao

et al. 2018

Front. Microbiol.

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“…The screening conditions were based on oral bioavailability (OB; ≥30%) and drug-likeness (DL ≥0.18), so as to obtain the drug composition and targets. Numerous recent studies have shown that the screening criteria can screen out effective pharmaceutical ingredients (19)(20)(21)(22).…”

Section: Methodsmentioning

confidence: 99%

Network pharmacological systems study of Huang‑Lian‑Tang in the treatment of glioblastoma multiforme

Liu

et al. 2020

Oncol Lett

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Glioblastoma multiforme (GBM) has a poor prognosis and its recurrence and mortality rates are high. At present, there is no effective clinical method to control its progression and recurrence. Traditional Chinese Medicine has a high status not only in China, but also in the world. Certain drugs are also used in the clinical treatment of tumor diseases. In clinical practice, Huang-Lian-Tang (HLT) has proven efficacy in treating brain diseases and preventing tumor recurrence. However, the mechanisms of action have remained elusive. The present study explored the potential mechanisms of HLT in the treatment of gliomas based on network pharmacology. First, information on the composition of HLT was obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, and the composition and targets of the chemical substances contained in the herbs were analyzed. Subsequently, a pharmacological interaction network for HLT was built. Furthermore, the expressed genes of patients with GBM were obtained from Gene Expression Omnibus database and screened. A protein-protein interaction network was then constructed for both sets of data and they were combined with a topology method for analysis. Finally, the screened genes were subjected to enrichment analysis and pathway analysis. A total of 386 candidate targets and 7 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were screened, which were mainly associated with amino acid metabolism. Gene Ontology enrichment analysis and KEGG signal pathway analysis indicated that these targets are involved in anti-apoptosis, anti-oxidative stress, multicellular biological processes and other physiological and pathological processes related to the occurrence and development of GBM. In conclusion, the present results indicated that the mechanisms of action of HLT against GBM involve multiple targets and signaling pathways that are related to tumorigenesis and progression. The present study not only provided a novel theoretical basis for Traditional Chinese Medicine to treat tumors but also novel ideas for the treatment of GBM.

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“…It is important to consider the relevance between the formula targets and disease genes for the elucidation of the associations between the formula and disease. The basic assumption is that if the formula is effective for the treatment of the disease, the formula targets are likely to be close to disease genes in the PPI network [ 55 ]. In this study, two network-based proximity measure approaches were used to capture such relatedness: the shortest path distance (SPD)[ 56 ] and a random walk with restart (RWR)[ 57 ] based scoring method.…”

Section: Methodsmentioning

confidence: 99%

A Network Pharmacology Approach to Uncover the Molecular Mechanisms of Herbal Formula Ban‐Xia‐Xie‐Xin‐Tang

Yang

Chen

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Ban-Xia-Xie-Xin-Tang (BXXXT) is a classical formula from Shang-Han-Lun which is one of the earliest books of TCM clinical practice. In this work, we investigated the therapeutic mechanisms of BXXXT for the treatment of multiple diseases using a network pharmacology approach. Here three BXXXT representative diseases (colitis, diabetes mellitus, and gastric cancer) were discussed, and we focus on in silico methods that integrate drug-likeness screening, target prioritizing, and multilayer network extending. A total of 140 core targets and 72 representative compounds were finally identified to elucidate the pharmacology of BXXXT formula. After constructing multilayer networks, a good overlap between BXXXT nodes and disease nodes was observed at each level, and the network-based proximity analysis shows that the relevance between the formula targets and disease genes was significant according to the shortest path distance (SPD) and a random walk with restart (RWR) based scores for each disease. We found that there were 22 key pathways significantly associated with BXXXT, and the therapeutic effects of BXXXT were likely addressed by regulating a combination of targets in a modular pattern. Furthermore, the synergistic effects among BXXXT herbs were highlighted by elucidating the molecular mechanisms of individual herbs, and the traditional theory of “Jun-Chen-Zuo-Shi” of TCM formula was effectively interpreted from a network perspective. The proposed approach provides an effective strategy to uncover the mechanisms of action and combinatorial rules of BXXXT formula in a holistic manner.

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Bioinformatics Analysis for the Antirheumatic Effects of Huang-Lian-Jie-Du-Tang from a Network Perspective

Cited by 24 publications

References 56 publications

Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Huang-Lian-Jie-Du-Decoction Ameliorates Hyperglycemia and Insulin Resistant in Association With Gut Microbiota Modulation

Network pharmacological systems study of Huang‑Lian‑Tang in the treatment of glioblastoma multiforme

A Network Pharmacology Approach to Uncover the Molecular Mechanisms of Herbal Formula Ban‐Xia‐Xie‐Xin‐Tang

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