Bioinformatics analysis of differentially expressed genes in human embryonic stem cells and their differentiated dorsal root ganglion

Abstract

To explore the difference of gene expression between undifferentiated hESCs and DRG induced by hESCs differentiation for 1 day by bioinformatics technology, and find out the key differential genes and their potential molecular regulation mechanism; The functions involved are analyzed and predicted. Download the gene chip data series GSE75582 related to stem cells and dorsal root ganglion from the geo expression database, import the gene chip online analysis tool GEO2R, screen out the differential genes, and construct the volcano map. Webgestalt database was used for GO function analysis and KEGG pathway enrichment analysis, and string analysis and Cytoscape software were used to construct PPI network. Results 4102 differentially expressed genes were screened, of which 2674 were up-regulated and 1428 were down regulated. Bioinformatics analysis of differentially expressed genes showed that the genes were mainly enriched in axon guidance, signaling pathways regulating pluripotency of stem cells, pathways in cancer, ECM receiver interaction, focal adhesion, cell adhesion molecules (CAMs), PI3K Akt signaling pathway, MAPK signaling pathway, TGF beta signaling pathway Ras signaling pathway, which extracts differential genes in stem cell related signaling pathways, including 34 up-regulated genes and 13 down regulated genes. Conclusion BMP4, Sox2, FGF2, Nanog, Smad3, KLF4, gsk3b and FGFR2 in undifferentiated hESCs are closely related to stem cell self-renewal; BMP4, Sox2, FGF2, Smad3, gsk3b and FGFR2 were mostly related to neuropathic pain and neurite growth. However, the functions of these key genes need to be further confirmed by future experimental studies.