Bioinspired gold nanoparticles-based colorimetric nanosensor for detection of antimony in plastic bottled water

Refera, Berhanu; Bekana, Deribachew; Temesgen, Ayalew; Teju, Endale; Jabessa, Abdisa

doi:10.1007/s11164-023-05037-z

Cited by 4 publications

(1 citation statement)

References 38 publications

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“…Currently, colorimetric methods deserve special attention as they offer a visual assessment of color change, allowing an easy readout of analytical signals. Colorimetric techniques for Sb 3+ detection using indicator dyes [14][15][16] and nanoparticles [17][18][19] were developed. The application of dyes such as bis [2-(5-chloro-2-pyridylazo)-5-diethylaminophenola to]cobalt(III), bromopyrogallol red and pyrene causes the formation of complexes with Sb 3+ and is followed by photo-or fluorometric detection.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Silver-Enhanced Microplate Apta-Enzyme Assay of Sb3+ Ions in Drinking and Natural Waters

Komova,

Serebrennikova,

Berlina

et al. 2023

Molecules

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The toxic effects of antimony pose risks to human health. Therefore, simple analytical techniques for its widescale monitoring in water sources are in demand. In this study, a sensitive microplate apta-enzyme assay for Sb3+ detection was developed. The biotinylated aptamer A10 was hybridized with its complementary biotinylated oligonucleotide T10 and then immobilized on the surface of polysterene microplate wells. Streptavidin labeled with horseradish peroxidase (HRP) bound to the biotin of a complementary complex and transformed the 3,3′,5,5′-tetramethylbenzidine substrate, generating an optical signal. Sb3+ presenting in the sample bounded to an A10 aptamer, thus releasing T10, preventing streptavidin-HRP binding and, as a result, reducing the optical signal. This effect allowed for the detection of Sb3+ with a working range from 0.09 to 2.3 µg/mL and detection limit of 42 ng/mL. It was established that the presence of Ag+ at the stage of A10/T10 complex formation promoted dehybridization of the aptamer A10 and the formation of the A10/Sb3+ complex. The working range of the Ag+-enhanced microplate apta-enzyme assay for Sb3+ was determined to be 8–135 ng/mL, with a detection limit of 1.9 ng/mL. The proposed enhanced approach demonstrated excellent selectivity against other cations/anions, and its practical applicability was confirmed through an analysis of drinking and spring water samples with recoveries of Sb3+ in the range of 109.0–126.2% and 99.6–106.1%, respectively.

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