Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Üzülmez, Betül; Demirsoy, Zeynep; Can, Özge; Gülseren, Gülcihan

doi:10.1002/mabi.202300057

Cited by 4 publications

(3 citation statements)

References 42 publications

(60 reference statements)

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“…Peptide cleavage and removal of the protecting groups were performed with 95:2.5:2.5 trifluoroacetic acid (TFA)/triisopropylsilane (TIS)/water for 2.5 h at room temperature. TFA was rotary-evaporated, and evaporation was followed by the addition of ice-cold diethyl ether for precipitating the resulting peptide yield. , …”

Section: Methodsmentioning

confidence: 99%

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Gülseren,

Demirsoy,

Şeker

et al. 2024

Mol. Pharmaceutics

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Unlocking the potential of metal nanoparticles (NPs) in biomedical applications represents a leading endeavor in contemporary research. Among these, gold NPs (AuNPs) and silver NPs (AgNPs) have shown promising strides in combatting complex neurodegenerative ailments like Alzheimer's disease. Yet, the unexplored realm of bimetallic Au/Ag-NP harbors immense potential, concealing undiscovered opportunities for enhanced therapeutic effectiveness through the synergistic interaction of metal ions. Nonetheless, the limitations of traditional synthesis methods have restricted the preparation, biocompatibility, and versatility of these NPs, prompting an urgent requirement for innovative approaches. Biobased synthetic methodologies have emerged as a noteworthy solution to address these challenges. Our study ventures into uncharted terrain, harnessing collagen-mimicking peptide nanofibers as a bioactive template for the synthesis of bimetallic NPs. These green NPs exhibit remarkable activity in inhibiting amyloid β (Aβ) protein aggregation with almost 74% inhibition, surpassing the individual impacts of Au and Ag NPs, which show inhibition percentages of 66 and 43, respectively. The bimetallic Au/Ag-NPs not only demonstrate powerful inhibition of Aβ, but they also demonstrate inhibitory activity against esterase (∼50%) and against reactive oxygen species (ROS) (∼75%), metamorphosing into multifaceted therapeutic agents for Alzheimer's disease. Au/Ag-NPs have proven highly beneficial in surpassing cellular barriers, as evidenced by studies on tissue penetration, 3D uptake, and endosomal escape, and these attributes also hold promise for the future treatment modalities. The findings indicate that the intrinsic traits of Au/Ag-NPs provide numerous mechanistic benefits, such as inhibiting Aβ and acetylcholinesterase (AChE), and reducing stress related to ROS, in addition to their advantageous internalization properties. This research represents a notable advancement in the development of multitargeted treatments for neurodegenerative disorders using bimetallic NPs, diverging from the prevalent emphasis on AuNPs in the current literature.

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Section: Methodsmentioning

confidence: 99%

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Gülseren,

Demirsoy,

Şeker

et al. 2024

Mol. Pharmaceutics

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“…At the end of incubation, 10 μL 10% Alamar blue solution prepared with 0.9% NaCl solution was added to each well and fluorescence quantification was made using a fluorescence microplate reader (BioTek Synergy H1 Hybrid Multi-Mode Reader) with an excitation wavelength of 560 nm and emission wavelength of 590 nm. The percent viability of the experimental groups was calculated by taking the viability of the control group as being 100% …”

Section: Materials and Methodsmentioning

confidence: 99%

“…The percent viability of the experimental groups was calculated by taking the viability of the control group as being 100%. 28 For the colony-counting assay, 2 × 10 4 bacterial cells were inoculated with 600, 300, and 150 nM AuNP@NisPec nanoparticles and incubated at 37 °C for 4 h with 250 rpm shaking. At the end of 4 h, samples were diluted 10 6 times and seeded onto TSA Petri dishes.…”

Section: Antibacterial Studiesmentioning

confidence: 99%

Ultrasmall Gold Nanospheres Engineered with Nisin Pectin Bioconjugate: A Multifunctional Solution for Dye Removal, Detoxification, and Antimicrobial Applications

Demirsoy,

Sara,

Aysan

et al. 2023

ACS Appl. Eng. Mater.

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Contamination of water sources with effluents like toxic dye molecules, organophosphorus (OP) compounds, and pathogenic bacteria is a major public concern, since those contaminants cause serious health problems in humans, animals, and plants. Thus, there is an urgent need for solutions to clean water sources from toxic and harmful pollutants. Gold nanoparticles exhibit remarkable potential for the remediation of contaminated water sources due to their essential catalytic and antimicrobial attributes. Nevertheless, the existing synthesized gold nanoparticles have not yet achieved the desired triple-action capability of simultaneously eliminating toxic dye molecules, organophosphorus (OP) compounds, and pathogenic bacteria. To address this challenge, we designed ultrasmall gold nanospheres that incorporate a bioconjugate of nisin antimicrobial peptide and pectin (NisPec). The resulting nanoparticles, termed AuNP@NisPec, demonstrated the ability to generate reactive oxygen species (ROS), facilitating the decolorization and detoxification of harmful dye molecules. The synergistic relationship between the gold nanospheres and the NisPec polymer allowed these nanoparticles to hydrolyze the phosphoester bond, rendering them a potential solution for OP toxicity. Additionally, the NisPec-decorated gold nanospheres exhibited antimicrobial activity against both Gram-positive and Gram-negative bacteria. This innovative approach offers a comprehensive and holistic solution to address water contamination by simultaneously tackling multiple challenges.

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Recent developments in functional organic polymer coatings for biomedical applications in implanted devices

Yang,

Jia,

Zhao

et al. 2024

Friction

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Organic polymer coatings have been commonly used in biomedical field, which play an important role in achieving biological antifouling, drug delivery, and bacteriostasis. With the continuous development of polymer science, organic polymer coatings can be designed with complex and advanced functions, which is conducive to the construction of biomedical materials with different performances. According to different physical and chemical properties of materials, biomedical organic polymer coating materials are classified into zwitterionic polymers, non-ionic polymers, and biomacromolecules. The strategies of combining coatings with substrates include physical adsorption, chemical grafting, and self-adhesion. Though the coating materials and construction methods are different, many biomedical polymer coatings have been developed to achieve excellent performances, i.e., enhanced lubrication, anti-inflammation, antifouling, antibacterial, drug release, anti-encrustation, anti-thrombosis, etc. Consequently, a large number of biomedical polymer coatings have been used in artificial lungs, ureteral stent, vascular flow diverter, and artificial joints. In this review, we summarize different types, properties, construction methods, biological functions, and clinical applications of biomedical organic polymer coatings, and prospect future direction for development of organic polymer coatings in biomedical field. It is anticipated that this review can be useful for the design and synthesis of functional organic polymer coatings with various biomedical purposes.

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Bioinspired Multi‐Layer Biopolymer‐Based Dental Implant Coating for Enhanced Osseointegration

Cited by 4 publications

References 42 publications

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Exploring Bimetallic Nanoparticles in Alzheimer’s Therapy: A Novel Bio-Assisted Synthesis with Multitarget Potential

Ultrasmall Gold Nanospheres Engineered with Nisin Pectin Bioconjugate: A Multifunctional Solution for Dye Removal, Detoxification, and Antimicrobial Applications

Recent developments in functional organic polymer coatings for biomedical applications in implanted devices

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