“…The impact of toxic compounds on activated sludge is in#uenced by several factors such as the acclimation of biomass to these substances (Chang et al, 1986), the sludge age of biomass (Lombrana et al, 1993), the concentration of biomass (Lamp and Tollefson, 1973), the length of exposure of biomass to the toxic compound (Madoni et al, 1999), the species of microorganisms present in activated sludge (Shuttleworth and Unz, 1991), and the pH (Villaescusa et al, 1997). Therefore, the e!ects of these factors on the determined toxicity should be investigated.…”