Biologic variability and correlation of platelet function testing in healthy dogs

Blois, Shauna L.; Lang, Sean; Wood, Richard D.; Monteith, Gabrielle

doi:10.1111/vcp.12290

Cited by 24 publications

(34 citation statements)

References 31 publications

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“…Additionally, CV G > CV I for all CAT parameters in the present study shows that thrombin generation among this dog population is variable. This finding is consistent with other studies examining the biological variability of hemostatic tests in dogs, where platelet function tests and TEG had high CV G . The utility of RIs for interpreting CAT can be further examined by calculating the index of individuality, which incorporates CV A , CV I , and CV G as previously reported .…”

Section: Discussionsupporting

confidence: 89%

“…CV A , CV G , and CV I were calculated by dividing their respective variance component by the square root of the log of the mean for any datasets that were log transformed. Maximum imprecision (CV Max ), maximum inaccuracy (B Max ), maximum total error (TE Max ), and indices of individuality, as well as the reference change value (RCV 95% ) were calculated for each variable using previously reported formulas …”

Section: Methodsmentioning

confidence: 99%

“…Based on these variance calculations, an index of individuality is calculated for each parameter. High indices of individuality, and therefore high biological variability, have been reported for most hemostatic and platelet function tests in dogs, but has not yet been reported for CAT in dogs. The purpose of this study was (1) to investigate the impact of preanalytical factors such as sample collection methods and storage time variation prior to running the CAT assay, and (2) to evaluate the biological variability of CAT in healthy dogs.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

Cuq

Blois

Wood

et al. 2018

Veterinary Clinical Pathol

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

Cuq

Blois

Wood

et al. 2018

Veterinary Clinical Pathol

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“…For example, in our study, we used the collagen/epinephrine PFA‐100 cartridge to assess drug‐induced platelet dysfunction. The collagen/epinephrine cartridge has a wide reference interval in dogs, and is susceptible to greater biological variation than the collagen/ADP PFA‐100 cartridge . The collagen/ADP cartridge could have detected some changes in platelet function that were not observed with the collagen/epinephrine cartridge, and the collagen/ADP cartridge could be used in future studies to assess the effects of immunosuppressive treatment on platelet function.…”

Section: Discussionmentioning

confidence: 99%

Effects of immunosuppressive agents on the hemostatic system in normal dogs

Thomason

Archer

Wills

et al. 2018

Veterinary Internal Medicne

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BackgroundIn dogs, the effects of immunosuppressive medications on hemostasis are not well known.Hypothesis/ObjectivesThe objective was to determine the effects of immunosuppressive medications on primary and secondary hemostasis. Our hypothesis was that cyclosporine and prednisone would increase markers of hypercoagulability and thromboxane synthesis, while azathioprine, mycophenolate mofetil, and leflunomide would have minimal effects on hemostasis.AnimalsEight healthy dogs.MethodsA randomized, cross‐over study used aggregometry, the PFA‐100 platelet function analyzer, viscoelastometry, platelet count, and prothrombin and activated partial thromboplastin times to evaluate hemostasis during the administration of prednisone, azathioprine, cyclosporine, mycophenolate mofetil, and leflunomide for 1 week each at standard oral doses. Urine 11‐dehydro‐thromboxane‐B2 (11‐dTXB2) and 6‐keto‐prostaglandin‐F1α (6‐keto‐PGF1α) concentrations, normalized to urine creatinine concentration, were measured.ResultsThe aggregometry amplitude decreased from 51 ± 21 to 27 ± 14 (P = .002) during leflunomide treatment (ADP activation), but there were no differences in amplitude (P = .240) for any medications when platelets were activated with collagen. For all medications, there were no significant differences in viscoelastometry indices (ACT, P = .666; ClotRate, P = .340; and platelet function, P = .411) and platelet count (P = .552). Compared with pretreatment values, urinary 11‐dTXB2‐to‐creatinine ratio increased (P = .001) after drug administration (from 3.7 ± 0.6 to 5.6 ± 1.1). Cyclosporine was associated with an increase (P < .001) in the 6‐keto‐PGF1α‐to‐creatinine ratio (from 10.3 ± 4.6 to 22.1 ± 5.3).Conclusions and Clinical ImportanceMost immunosuppressive drugs do not enhance platelet function or coagulation in healthy dogs, suggesting that these medications might not predispose hypercoagulable dogs to thromboembolism. The results of our study need to be correlated with the clinical outcomes of hypercoagulable dogs.

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“…No such correlation was observed when ARA was used as the inducer of platelet aggregation in LTA testing. This suggests that the difference between impedance aggregometry and LTA shows different predictive values for various platelet function tests [25, 26]. Several reports have shown that analgesic therapy with NSAIDs have a significant effect on the efficacy of aspirin [10-14].…”

Section: Discussionmentioning

confidence: 99%

Role of Dipyrone in the High On-Treatment Platelet Reactivity amongst Acetylsalicylic Acid-Treated Patients Undergoing Peripheral Artery Revascularisation

et al. 2018

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Objective: To evaluate the effects of dipyrone on sensitivity to aspirin (acetylsalicylic acid [ASA]) in patients who underwent peripheral artery vascular reconstruction. Subjects and Methods: Impedance aggregometry and light transmission aggregometry were used to determine the effects of dipyrone on ASA treatment in 21 patients. Blood samples were drawn in a 7-day period after the surgery. The cut-off value for high on-treatment platelet reactivity (HTPR) was set at < 65% of aggregation inhibition for impedance aggregometry. For light transmission aggregometry the cut-off value for arachidonic acid-induced aggregation was set at > 20% of aggregating platelets, and the cut-off value for epinephrine-induced aggregation was > 44% of aggregating platelets. The cut-off value for each method was derived from a large number of patients treated with a daily dose of 100 mg of ASA. Results: We found HTPR in 14 (67%) of the 21 patients. None had primary resistance to ASA, i.e., after the addition of ASA in vitro all samples showed antiplatelet efficacy. Regression analysis showed a possible correlation between lower efficacy of ASA treatment and higher daily doses of dipyrone (p = 0.005 for impedance aggregometry, p = 0.04 for light transmission aggregometry), higher platelet count (p = 0.005 for impedance aggregometry), and shorter time from surgery (p = 0.03 for impedance aggregometry). Conclusion: HTPR occurs in 67% of ASA-treated patients after lower limb vascular surgery. The occurrence of HTPR correlates with the daily dose of dipyrone. Therefore, dipyrone should not be used as a postoperative analgesic in ASA-treated patients after peripheral artery revascularisation due to its influence on the effectiveness of ASA.

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Biologic variability and correlation of platelet function testing in healthy dogs

Cited by 24 publications

References 31 publications

Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

Reproducibility, stability, and biological variability of thrombin generation using calibrated automated thrombography in healthy dogs

Effects of immunosuppressive agents on the hemostatic system in normal dogs

Role of Dipyrone in the High On-Treatment Platelet Reactivity amongst Acetylsalicylic Acid-Treated Patients Undergoing Peripheral Artery Revascularisation

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