Biological activities and DNA interactions of aqueous extract of Phlomis linearis (Boiss. & Bal.)

Abstract

Phlomis linearis Boiss. & Bal. of the Lamiaceae family is one of the endemic species in Turkey, i.e., growing in the east, central, and southeast parts of Anatolia and used for herbal tea. This study was designed to identify the biochemical and bioactivity properties of this endemic species by DPPH scavenging activity, metal chelating activity, total phenolic content, HPLC-DAD analysis, and MTT assay. Furthermore, the plant extract was evaluated for its antimicrobial activity against bacteria and fungi by using the microdilution method. The interactions between extract and plasmid DNA and their restriction endonuclease reactions were investigated by agarose gel electrophoresis. To support our hypothesis, we performed a molecular docking analysis. The DPPH scavenging activity of the plant extract was 53.86 ± 0.50 µg/ml in terms of IC50 value. The IC50 value of the plant extract was determined as 14.71 ± 4.01 mg/ml for metal chelating assay. The phenolic content of the extract was 231.55 ± 2.11 mg/g dry weight expressed as gallic acid equivalents (GAE). HPLC-DAD results revealed that the phenolic compounds were mainly derivatives of rosmarinic acid, chlorogenic acid, luteolin, luteolin-7-glycoside, luteolin derivatives, rutin derivatives, and apigenin derivatives. Besides, the cytotoxic activity of the plant extract against L929 fibroblast, H1299 non-small-cell lung carcinoma, and Caco-2 colorectal adenocarcinoma cell lines was determined by MTT assay. Phenolic content and molecular docking results correlated with each other.

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Bioactive Compounds of Rosa canina L. and Their Effect on Tumor Necrosis Factor-α and Interleukin-1β Activity in Diabetes-Induced Rats

“…DNA damage inhibition potential of MRE and WRE of R. canina was evaluated using plasmid DNA. Oxidative damage to plasmid DNA was induced using hydroxyl (•OH) radical generated from the ultraviolet (UV)/H 2 O 2 -radical system as described (24). A reaction mixture containing 20 μL of plant extract of different concentrations (25-50 μg/mL), pBR322 DNA (0.25 μg), and 20 μL Fenton's reagent (30 mmol/L H 2 O 2 , 500 μmol/ L-ascorbic acid and 800 μmol /L FeCl3 was incubated for 40 min at 37°C and analyzed on a 1% agarose gel by staining with ethidium bromide.…”