Biological activities and physicochemical characterization of alkaline lignins obtained from branches and leaves of Buchenavia viridiflora with potential pharmaceutical and biomedical applications

Araújo, Denise Maria Figueiredo; Filho, Iranildo José da Cruz; Santos, Tiago Felipe de Abreu; Pereira, Daniel Tarciso Martins; Marques, Denílson Bezerra; Lima, Alice da Conceição Alves de; Aquino, Thiago Mendonça de; Rocha, George Jackson de Moraes; Lima, Maria do Carmo Alves de; Nogueira, Fátima

doi:10.1016/j.ijbiomac.2022.07.225

Cited by 11 publications

(13 citation statements)

References 91 publications

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“…The antimalarial activity was estimated by previously described protocols with adjustments in at least two experiments, each in triplicate [53,54]. In short, unsynchronized cultures with 2% hematocrit and 1% parasitemia of P. falciparum 3D7-GFP and Dd2 strains were incubated for 72h (37 °C and 5% CO2) in a 96-well flat-bottom plate with BITC in 2-fold serial dilutions ranging from 0.50 μg/ml to 0.0005 μg/ml (3.32 μM to 0.0032 μM).…”

Section: Dose-response Evaluationmentioning

confidence: 99%

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Pintão,

Santos,

Nogueira

2024

Preprint

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Malaria remains an important and challenging infectious disease, and novel antimalarials are required. Benzyl isothiocyanate (BITC), the main breakdown product of benzyl glucosinolate, is present in all parts of Tropaeolum majus L. (T. majus) and has antibacterial and antiparasitic activities. To our knowledge, there is no information on the effects of BITC against malaria. The present study evaluates the antimalarial activity of BITC and T. majus seeds, leaves, and stems aqueous extracts. We used flow cytometry to calculate the growth inhibition (GI) percentage of the extracts and BITC against unsynchronized cultures of chloroquine-susceptible Plasmodium falciparum (P. falciparum) 3D7-GFP strain. Extracts and/or compounds with at least 70% GI were validated by IC50 estimation against P. falciparum 3D7-GFP and Dd2 (chloroquine-resistant strain) unsynchronized cultures by flow cytometry, and the resistance index (RI) was determined. T. majus aqueous extracts showed some antimalarial activity higher in seeds than in leaves or stems. BITC’s GI was comparable to chloroquine’s. BITC’s IC50 was similar in both strains, thus a cross-resistance absence with aminoquinolines was found (RI < 1). BITC presented features that could open new avenues for malaria drug discovery.

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Section: Dose-response Evaluationmentioning

confidence: 99%

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Pintão,

Santos,

Nogueira

2024

Preprint

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“…A saline solution was the negative control 0% hemolysis and triton X was the positive control 100% hemolysis. All experiments were performed in triplicate (Araujo et al 2022).…”

Section: In Vitro Hemolytic Activitymentioning

confidence: 99%

“…Lignins are described as promising antimicrobial agents (Melo et al 2020, Araujo et al 2022. Table III presents the results of in vitro antimicrobial activity promoted by lignin from Morinda citrifolia leaves against different species of bacteria and fungi.…”

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

“…However, it is known that lignins that present greater amounts of phenolic fragments containing a C-C double bond in the α and β positions of the side chain and a methyl group in the γ position promote greater inhibition of microbial growth when compared to lignins that present higher levels of phenolic fragments with functional groups containing oxygen (-OH, -CO, COOH) in the side chain (Melo et al 2020, Araujo et al 2022. Araujo et al (2022) evaluating alkaline lignins obtained from the branches and leaves of Buchenavia viridiflora verified that these were able to promote antimicrobial activity, presenting a minimum inhibitory concentration of 512 μg/mL for the bacteria Enterococcus faecalis UFPEDA-69, Staphylococcus aureus UFPEDA-709, Pseudomonas aeruginosa UFPEDA-261 and Candida albicans 4664 yeast. Furthermore, the authors observed that none of the evaluated lignins showed minimal bactericidal or fungicidal concentration.…”

Section: In Vitro Antimicrobial Activitymentioning

confidence: 99%

“…However, when recovered, they can be used as raw material to obtain products with high added value (Agustiany et al 2022). The literature has presented several applications for lignins, among them applications related to biomedical areas (Sugiarto et al 2022), such as antioxidant, antitumor (Araujo et al 2022), immunostimulant (Cruz Filho et al 2019, Nascimento Santos et al 2020), antiparasitic (Silva et al 2021), antiviral (Kimura et al 2022 among others. And all these applications are directly related to the chemical structure (Kimura et al 2022, Sugiarto et al 2022.…”

Section: Introductionmentioning

confidence: 99%

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Alkaline lignins from Morinda citrifolia leaves are potential immunomodulatory, antitumor, and antimicrobial agents

CRUZ FILHO,

REIS,

NASCIMENTO

et al. 2023

An. Acad. Bras. Ciênc.

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Morinda citrifolia, commonly known as noni, is a plant belonging to the Rubiaceae family. This plant has a high biological potential, which has different biological properties, including antioxidant, antibacterial, antiviral, antifungal, antitumor and anti-inflammatory. In this work, the immunomodulatory, antitumor and antimicrobial activities of lignin isolated from Morinda citrifolia leaves were investigated. The results showed that this lignin was not cytotoxic and that it was able to promote activation and differentiation of immune cells in addition to inducing the production of antiinflammatory cytokines. Furthermore, it was able to inhibit the growth of different tumor and microbial cells in vitro. This pioneering study on these different activities shows that the lignin isolated in this study can be used as a raw material to obtain biomedical and pharmaceutical products.

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Evaluation of the hydroalcoholic extract of Clarisia racemosa as an antiparasitic agent: an in vitro approach

da Cruz Filho,

Duarte,

Marques

et al. 2023

3 Biotech

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Biological activities and physicochemical characterization of alkaline lignins obtained from branches and leaves of Buchenavia viridiflora with potential pharmaceutical and biomedical applications

Cited by 11 publications

References 91 publications

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Antimalarial Activity of Aqueous Extracts of Nasturtium (Tropaeolum majus L.) and Benzyl Isothiocyanate

Alkaline lignins from Morinda citrifolia leaves are potential immunomodulatory, antitumor, and antimicrobial agents

Evaluation of the hydroalcoholic extract of Clarisia racemosa as an antiparasitic agent: an in vitro approach

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