Biological Activities of Aerial Parts Extracts of<i>Euphorbia characias</i>

Pisano, Maria Barbara; Cosentino, Sofia; Viale, Silvia; Spanò, Delia; Corona, Angela; Esposito, Francesca; Tramontano, Enzo; Montoro, Paola; Tuberoso, Carlo Ignazio Giovanni; Medda, Rosaria; Pintus, Francesca

doi:10.1155/2016/1538703

Cited by 35 publications

(45 citation statements)

References 46 publications

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“…Total content of polyphenols and flavonoids in the extracts was determined as previously reported [21]. Polyphenol concentration was calculated using gallic acid as a referred standard and was expressed as mg of gallic acid equivalent (GAE) per 1 g of dry weight (dw).…”

Section: Methodsmentioning

confidence: 99%

Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts

Petrillo

González-Paramás

Era

et al. 2016

BMC Complement Altern Med

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110

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Background Asphodelus microcarpus belongs to the family Liliaceae that include several medicinal plants. In the traditional medicine plants of the genus Asphodelus are used to treat skin disorders such as ectodermal parasites, psoriasis, microbial infection and for lightening freckles. In order to find novel skin depigmenting agents, the present work was carry out to evaluate antioxidant activity and tyrosinase inhibitory potential of leaves, flowers and tubers extracts of A. microcarpus. The phytochemical composition of the active extract was also evaluated.MethodsThree different extracts (water, methanol and ethanol) from leaves, flowers and tubers of A. microcarpus were evaluated for their inhibitory effect on tyrosinase activity using l-3,4-dihydroxyphenylalanine (l-DOPA) as substrate. Inhibition of cellular tyrosinase activity and melanin production was also investigated in melanoma B16F10 cells. Antioxidant activity, total phenolic and flavonoids contents were determined using standard in vitro methods. HPLC-DAD-MS was used to identify phenolic profile of the active extract.ResultsThe results showed that all extracts have a direct inhibitory anti-tyrosinase activity, with ethanolic extract from flowers (FEE) exhibiting the stronger effect. Kinetic analysis revealed that FEE acts as an uncompetitive inhibitor with a Ki value of 0.19 mg/mL. The same effect was observed in murine melanoma B16F10 cells. Cellular tyrosinase activity as well as melanin content were reduced in FEE-treated cells. The results were comparable to that of the standard tyrosinase inhibitor (kojic acid). Furthermore, the same extract showed the highest antioxidant activity and an elevated levels of total phenolics and flavonoid content. Eleven phenolic components were identified as chlorogenic acid, luteolin derivates, naringenin and apigenin.ConclusionsOur findings showed that FEE from A. microcarpus inhibits tyrosinase and exerted antimelanogenesis effect in B16F10 cells. This extract also showed the highest scavenging activity, which could be mainly attributed to its high levels of total polyphenols and flavonoids. These results suggest that A. microcarpus has a great potential as sources of bioactive compounds which could be used as depigmenting agents in skin disorders.

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Section: Methodsmentioning

confidence: 99%

Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts

Petrillo

González-Paramás

Era

et al. 2016

BMC Complement Altern Med

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110

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“…Other fragment ions at m/z 149 and 134, formed after the loss of a methyl group, was led to the identification of this peak as 3-feruloylquinic acid (1) [23]. Compounds 2-4 were determined as quercetin derivatives which were previously identified in Euphorbia species [24]. Compound 2 showed pseudo molecular ion peak at m/z 463 and a base peak ion at m/z 300 which was formed after the loss of a glucose unit.…”

Section: Resultsmentioning

confidence: 96%

Chemical Composition and Mosquitocidal Activity of n-Hexane and Methanolic Extracts from Euphorbia anacampseros var. tmolea: An Endemic Species of Turkey against Aedes aegypti

Demiray¹,

Tabanca²,

Göğer³

et al. 2017

Asian J. Chem.

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Volatile composition of the n-hexane and methanol extracts from E. anacampseros var. tmolea was analyzed by head space-solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Chemical characterization of the methanolic extract was determined by LC-ESI-MS/MS. Both extracts were bioassayed against 1 st instar larvae and adult female Ae. aegypti. The main components identified from the E. anacampseros var. tmolea n-hexane fraction were 1,8-cineole (27.5 %), p-cymene (25 %), γ-terpinene (12.8 %), limonene (9.9 %). Methyl hexanoate (18.8 %), methyl nonanoate (13.3 %), dimethyl succinate (6.5 %), methyl octanoate (6 %) and methyl phenylacetate (5.3 %) were identified from the methanolic fraction. The n-hexane extract showed 100 % mortality at 0.1 µg/ µL against 1 st instar larvae of Ae. aegypti and the methanolic extract exhibited 83.3 % mortality at 5 µg/mosquito against adult female Ae. aegypti. The bioassay-guided study demonstrates that n-hexane and methanol extracts of E. anacampseros var. tmolea contain compounds with natural mosquito larvicidal and adulticidal activity.

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“…The 96-well microplates were incubated aerobically at 28 • C, the results were measured after 72 h. MIC was defined as the lowest concentration that showed a visual turbidity of less than or equal to 80% inhibition compared with that produced by the control well. The lowest concentration of the antifungal substance that killed ≥99.9% of the initial inoculum was defined as the MFC end point (Pisano et al, 2016).…”

Section: Antifungal Activity Assaymentioning

confidence: 99%

Identification of the Potential Biological Preservative Tetramycin A-Producing Strain and Enhancing Its Production

Ding

et al. 2020

Front. Microbiol.

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The aim of this study was to develop a potential microbial preservative to prevent the growth of fungi in food. The isolate ZC-G-5 showed strong antifungal activity against food spoilage fungi and Streptomyces albulus was identified on the basis of morphologic, culture, and 16S rDNA sequence analyses. The active metabolite was elucidated as tetramycin A (TMA) through spectroscopic techniques, including HR-ESI-MS, 1D-NMR, and 2D-NMR. An antifungal activity assay revealed that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of TMA were 1.50-2.50 and 3.00-5.00 µg/ml, respectively. In situ antifungal activity analyses demonstrated that 90.0 µg/ml of TMA could inhibit the growth of fungi for over 14 days. In order to enhance TMA production, the high-yield mutant strain YB101 was screened, based on the isolate ZC-G-5, using a high-throughput screening method. The best metabolic precursor was selected during fermentation, when the concentration of glycerol was 8% (v/v) in Gauze's broth medium to cultivate the mutant strain YB101; the concentration of TMA could be increased to 960.0 µg/ml, compared with the original isolate ZC-G-5, where the concentration of the TMA was only 225.0 µg/ml. Our study may contribute to the application of S. albulus and its active metabolite as a potential bio-preservative in the food industry.

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Biological Activities of Aerial Parts Extracts ofEuphorbia characias

Cited by 35 publications

References 46 publications

Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts

Tyrosinase inhibition and antioxidant properties of Asphodelus microcarpus extracts

Chemical Composition and Mosquitocidal Activity of n-Hexane and Methanolic Extracts from Euphorbia anacampseros var. tmolea: An Endemic Species of Turkey against Aedes aegypti

Identification of the Potential Biological Preservative Tetramycin A-Producing Strain and Enhancing Its Production

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