Biological and RNA regulatory function of MOV10 in mammalian germ cells

Abstract

Background RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous RNA helicases implicated in distinct intracellular pathways. MOV10L1 participates specifically in Piwi-interacting RNA (piRNA) biogenesis and protects mouse male fertility. In contrast, the functional complexity of MOV10 remains incompletely understood, and its role in the mammalian germline is unknown. Here, we report a study of the biological and molecular functions of the RNA helicase MOV10 in mammalian male germ cells. Results MOV10 is a nucleocytoplasmic protein mainly expressed in spermatogonia. Knockdown and transplantation experiments show that MOV10 deficiency has a negative effect on spermatogonial progenitor cells (SPCs), limiting proliferation and in vivo repopulation capacity. This effect is concurrent with a global disturbance of RNA homeostasis and downregulation of factors critical for SPC proliferation and/or self-renewal. Unexpectedly, microRNA (miRNA) biogenesis is impaired due partially to decrease of miRNA primary transcript levels and/or retention of miRNA via splicing control. Genome-wide analysis of RNA targetome reveals that MOV10 binds preferentially to mRNAs with long 3′-UTR and also interacts with various non-coding RNA species including those in the nucleus. Intriguingly, nuclear MOV10 associates with an array of splicing factors, particularly with SRSF1, and its intronic binding sites tend to reside in proximity to splice sites. Conclusions These data expand the landscape of MOV10 function and highlight a previously unidentified role initiated from the nucleus, suggesting that MOV10 is a versatile RBP involved in a broader RNA regulatory network. Electronic supplementary material The online version of this article (10.1186/s12915-019-0659-z) contains supplementary material, which is available to authorized users.

Identification of genes involved in inbreeding depression of reproduction in Langshan chickens

“…In addition to PTGDS, MOV10L1, and DEUP1 were also reported to be specifically expressed in testicular tissue [ 19 , 20 ]. The expressions of MOV10L1 and DEUP1 between the two chicken groups were extremely different, and they were in the top five ranked by FDR values.…”

“…The expressions of MOV10L1 and DEUP1 between the two chicken groups were extremely different, and they were in the top five ranked by FDR values. MOV10L1 participates in piwi-interacting RNA biogenesis and protects mouse male fertility, enhancing the proliferation capacities of spermatogonial progenitor cells [ 19 ]. A study on human tissue-specific expression reported that DEUP1 was specifically highly expressed in testicular tissues, indicating DEUP1 played an important role in male fertility [ 20 ].…”

“…The results of KEGG enrichment analysis showed that DEGs were significantly enriched in 10 pathways (P<0.05) ( Figure 43b, Table S6). These pathways In addition to PTGDS, MOV10L1 and DEUP1 were also reported to be specifically expressed in testicular tissue [1925,206]. The expressions of MOV10L1 and DEUP1 between the two chicken groups were extremely different, and they were in the top five ranked by FDR values.…”

Roles of MOV10 in Animal RNA Virus Infection

“…In the non-canonical pathway, pre-miRNAs are formed by splicing, debranching, and trimming of short introns without Drosha processing (74). In this process to form pre-miRNAs, MOV10 typically binds splicing factors, such as SRSF1 and DDX5 (68,75), forming a splicing complex, which completes the pre-mRNA and pri-miRNA splicing process after binding intronic regions (Figure 2).…”

Caracterização de um novo RNA longo não codificador e seu provável papel no desenvolvimento neuronal

“…Um importante mecanismo de ação dos lncRNAs é a sua capacidade de interação com proteínas e, uma vez identificadas essas interações, é possível compreender as potenciais funções dos lncRNAs(ZHANG et al, 2019a). Para tal análise, foi utilizado o banco de dados NPInter 4.0, que possui interações de ncRNAs validadas experimentalmente(TENG et al, 2020), encontrando interação do RP11-1082A3.1 com 6 proteínas (Tabela 2).As proteínas identificadas até o momento como parceiras de interação do lncRNA, de acordo com o os bancos de dados utilizados, estão localizadas principalmente no núcleo celular e estão, na Estudo recente demonstrou que a proteína MOV10 pode ser encontrada tanto no núcleo quanto no citoplasma de células germinativas masculinas primordiais, apresentando um papel fundamental na determinação de linhagem e na proliferação de células progenitoras espermatogoniais(FU et al, 2019). Além disso, um outro estudo, em camundongos, mostrou que essa proteína é essencial para o desenvolvimento embrionário e para o desenvolvimento e funcionamento saudável do sistema nervoso central(SKARIAH et al, 2017).…”