Biological characteristics, bioactive compounds, and antioxidant activities of off-season mulberry fruit

Liu, Peigang; Zhu, Yan; Ye, Jingjing; Lin, Tianbao; Lv, Zhiqiang; Xu, Zi-Long; Xu, Lushan; Chen, Leyang; Wang, Jia

doi:10.3389/fpls.2022.1034013

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Mulberry ( Morus atropurpurea ) belongs to the Moraceae family and is considered the sole crop of economic importance in sericulture [ 8 ], and its various parts (leaves, stems and roots) are used in the preparation of various products for the pharmaceutical, food, cosmetic and health care industries [ 9 , 10 ]. As an important economic forest species, mulberry is able to adapt to adverse abiotic stress conditions, including salinity, flooding, drought and metal stress, making it an excellent material for woody plant stress tolerance studies [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Wang,

Jiang,

et al. 2024

BMC Plant Biol

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Seed propagation is the main method of mulberry expansion in China, an important economic forest species. However, seed germination is the most sensitive stage to various abiotic stresses, especially salinity stress. To reveal the molecular regulatory mechanism of mulberry seed germination under salt stress, flavonoid metabolomics and transcriptomics analyses were performed on mulberry seeds germinated under 50 and 100 mmol/L NaCl stress. Analysis of the flavonoid metabolome revealed that a total of 145 differential flavonoid metabolites (DFMs) were classified into 9 groups, 40 flavonols, 32 flavones, 16 chalcones and 14 flavanones. Among them, 61.4% (89) of the DFMs accumulated continuously with increasing salt concentration, reaching the highest level at a 100 mmol/L salt concentration; these DFMs included quercetin-3-O-glucoside (isoquercitrin), kaempferol (3,5,7,4'-tetrahydroxyflavone), quercetin-7-O-glucoside, taxifolin (dihydroquercetin) and apigenin (4',5,7-trihydroxyflavone), indicating that these flavonoids may be key metabolites involved in the response to salt stress. Transcriptional analysis identified a total of 3055 differentially expressed genes (DEGs), most of which were enriched in flavonoid biosynthesis (ko00941), phenylpropanoid biosynthesis (ko00940) and biosynthesis of secondary metabolites (ko01110). Combined analysis of flavonoid metabolomic and transcriptomic data indicated that phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), flavonol synthase (FLS), bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase (DFR) and anthocyanidin reductase (ANR) were the key genes involved in flavonoid accumulation during mulberry seed germination under 50 and 100 mmol/L NaCl stress. In addition, three transcription factors, MYB, bHLH and NAC, were involved in the regulation of flavonoid accumulation under salt stress. The results of quantitative real-time PCR (qRT‒PCR) validation showed that the expression levels of 11 DEGs, including 7 genes involved in flavonoid biosynthesis, under different salt concentrations were consistent with the transcriptomic data, and parallel reaction monitoring (PRM) results showed that the expression levels of 6 key enzymes (proteins) involved in flavonoid synthesis were consistent with the accumulation of flavonoids. This study provides a new perspective for investigating the regulatory role of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress at different concentrations.

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Section: Introductionmentioning

confidence: 99%

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Wang,

Jiang,

et al. 2024

BMC Plant Biol

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“…Greenhouse cultivation is an effective and widely utilized technology that provides plants with optimally controlled microclimate growth conditions, extending the production season and protecting against diseases and insects. Greenhouses are predominantly used for off-season fruit and early spring fruit production [9][10][11]. Therefore, information on the agronomic characteristics of mulberry trees under greenhouse conditions is necessary for producers to understand the cultivars that are most ideally suited to their applications.…”

Section: Introductionmentioning

confidence: 99%

Multivariate Analysis of the Phenological Stages, Yield, Bioactive Components, and Antioxidant Capacity Effects in Two Mulberry Cultivars under Different Cultivation Modes

Zhang,

Li,

Qiu

et al. 2023

Horticulturae

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Mulberry fruits are rich in bioactive components renowned for their antioxidant properties and potential health benefits. This study thoroughly investigated the impact of cultivation modes on the phenological stages, yield, bioactive components, and antioxidant activity of two mulberry cultivars, Yueshen Dashi (YS-DS) and Xinjiang Baisang (XJ-BS). Notably, greenhouse cultivation led to earlier phenology and shorter fruit development maturation durations compared to field cultivation. Despite a decrease in fruit production and firmness, the greenhouse-grown mulberries exhibited higher individual fruit fresh weight. The content of bioactive components, encompassing anthocyanins, polyphenols, flavonoids, and vitamin C, and of antioxidant activity (measured in the FRAP and DPPH radical scavenging assays) was found to be lower in the greenhouse-grown mulberries than in those cultivated in the field. The contents of total polyphenols and flavonoids showed robust positive correlations in the FRAP and DPPH radical scavenging assays, which suggests that the antioxidant activity of mulberry fruit might be primarily attributable to the bioactive components of total polyphenols and flavonoids. Interestingly, the sugar content and hydroxyl radical scavenging activity (HRSA) displayed an inverse relationship between the two cultivars in the greenhouse versus field conditions. The multivariate analysis highlighted distinct patterns for different cultivars under varying cultivation modes. This study underscores the potential to enhance bioactive components and antioxidant activity through effective manipulation of climate conditions, thereby unlocking the full nutritional potential of mulberry fruits on a large scale in greenhouse environments.

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Bioaffinity ultrafiltration combined with UPLC-ESI-QTrap-MS/MS for screening of xanthine oxidase inhibitors from Paederia foetida L. leaves

Liu,

Hu,

et al. 2024

Arabian Journal of Chemistry

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Biological characteristics, bioactive compounds, and antioxidant activities of off-season mulberry fruit

Cited by 4 publications

References 36 publications

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress

Multivariate Analysis of the Phenological Stages, Yield, Bioactive Components, and Antioxidant Capacity Effects in Two Mulberry Cultivars under Different Cultivation Modes

Bioaffinity ultrafiltration combined with UPLC-ESI-QTrap-MS/MS for screening of xanthine oxidase inhibitors from Paederia foetida L. leaves

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