Biological characterization of oxidized hyaluronic acid/resveratrol hydrogel for cartilage tissue engineering

Abstract

Osteoarthritis is a type of arthritis that is caused by breakdown of cartilage, with eventual loss of the cartilage of the joints. The ability of self-repair in damaged cartilage tissue is limited; the aim of this work is to fabricate and characterize an oxidized hyaluronic acid/resveratrol (Oxi-HA/Res) hydrogel for future applications in cartilage tissue engineering. Under physiological conditions, the Oxi-HA/Res hydrogel was prepared by chemical crosslinking of Oxi-HA with resveratrol solution and characterized by Fourier transform infrared spectrometry assay; the biocompatibility and gene expression of chondrocytes within the Oxi-HA-Res hydrogel then analyzed. The cell viability and cytotoxicity assays showed that the Oxi-HA/Res hydrogel has good biocompatibility. Oxi-HA/Res hydrogel can upregulate expression of type II collagen, aggrecan, and Sox-9 genes; while down-regulating IL-1β, MMP-1, MMP-3, MMP13 gene expression. It can also reduce LPS-induced inflammation and chondrocyte damage. The results of this study showed that the Oxi-HA/Res hydrogel is biocompatible with chondrocytes, allows for extracellular matrix synthesis, and also reduce LPS-induced inflammation and damage. These results suggest that Oxi-HA/Res hydrogel may be a potential suitable cell carrier for chondrocyte cells in the treatment of cartilage defect. However, further in vivo study is mandatory for future possible clinical applications.

An anti-inflammatory cell-free collagen/resveratrol scaffold for repairing osteochondral defects in rabbits

“…For these reasons, it is of clinical significance to find natural remedies to inhibit the release of these pro-inflammatory cytokines. In this research, antiinflammatory scaffolds (Coll/PAA-Res) showed an enhanced ability to capture DPPH free radicals, and eventually offered a protective effect on the chondrocytes and BMSCs in a ROS environment, as reported previously [16,20]. Then, an acute trauma defect in the rabbit joint was created to evaluate the anti-inflammatory effect of the Coll/PAA-Res scaffold.…”

“…S.2) suggested that the Res was incorporated into Coll. Compared with the pure Coll scaffold, the peaks at 1147 and 950 cm À1 separately attributable to the CAO stretch of phenol and the CAH stretch of the benzene ring in Res [16,25] appear in Coll/PAA-Res.…”

“…The clinical results showed that the inhibition of IL-1b could reduce pain and swelling of patients with RA [15]. Recently, resveratrol (trans-3,5,4 0 -trihydroxystilbene, Res), a natural polyphenolic compound, has been shown to have anti-inflammatory, antioxidant, antiaging, anticarcinogenic and immunmodulatory properties [16]. Res has demonstrated anti-inflammatory effects through inhibition of TNF-a, IL-1b and COX-2 by blocking NF-jB activation [17][18][19]; it can inhibit the IL-1b-induced degradation of mitochondria and apoptosis in chondrocytes and bone marrow stromal stem cell (BMSC)-derived chondrocytes [20,21].…”

“…Therefore, preventing the rapid metabolism of Res is essential for its application. Sheu et al fabricated oxidized hyaluronic acid (Oxi-HA) hydrogel with chemically bonded Res, which was shown to protect the chondrocytes against lipopolysaccharide (LPS) [16]. Li et al used Res to modify the surface of porous polycaprolactone (PCL).…”

Significance of Epigenetic Landscape in Cartilage Regeneration from the Cartilage Development and Pathology Perspective

“…Resveratrol exerts its chondroprotective function in human chondrocytes in vitro by deactivating p53-induced apoptosis [165] or inhibiting IL-1b-induced apoptosis [166]. Evidence shows that oxidized hyaluronic acid/resveratrol hydrogel is a promising cell carrier for chondrocytes to repair cartilage defects [167].…”