Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Ji, Xiaodong; Lu, Guobing; Gai, Yingping; Zheng, Chengchao; Mu, Zhimei

doi:10.1111/j.1574-6941.2008.00543.x

Cited by 145 publications

(85 citation statements)

References 41 publications

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“…subtilis was the other bio-control agent of this research. There are many studies showing that Bacillus occurring naturally on the surface of fruits or vegetables is used as a bio-control agent of postharvest diseases (Kotan et al 2009;Elshafei et al 2012;Ketabchi et al 2012;Mohammadi et al (Nagorska et al 2007;Ji et al 2008;Francis et al 2010). The bacteria of the genus Bacillus have a a great potential as a biological control agent because they keep their viability with long-term storage (Nagorska et al 2007;Ongena & Jacques 2008).…”

Section: Resultsmentioning

confidence: 99%

Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

Mohammadi¹,

Tozlu²,

Kotan³

et al. 2017

Plant Prot. Sci.

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Mohammadi P., Tozlu E., Kotan R., Kotan Şenol M. (2017): Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum. Plant Protect. Sci., 53: 134-143.Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum.

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Section: Resultsmentioning

confidence: 99%

Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

Mohammadi¹,

Tozlu²,

Kotan³

et al. 2017

Plant Prot. Sci.

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“…Various biocontrol agents are used to control the bacterial wilt caused by R. solanacearum. Trichoderma harzianum 10,11 , Trichoderma viride 12 , Trichoderma asperellum 13 , Trichoderma virens 14 , Pseudomonas fluorescence 15,16 and Bacillus subtilis 17 are used as biocontrol agents to control bacterial wilt. Combination treatment methods using two or more of these agents are more effective in managing the disease than treatment using a single biocontrol agent 10,18,19 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Trichoderma spp., Pseudomonas fluorescence and Bacillus subtilis for biological control of Ralstonia wilt of tomato

Yendyo

G.C.²,

Pandey³

2017

F1000Res

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Abstractis the major bacterial disease in tomato, Background: Ralstonia solanacearum which invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions. R.produces several toxins helping it to invade the plant's natural solanacearum defense mechanism. Native isolates of Trichoderma spp., Pseudomonas and can be used as biocontrol agents to control fluorescence Bacillus subtilis the bacterial wilt and combined application of these beneficial microbes can give better results.Bacterial wilt infection in the field was identified by field experts and Methods: the infected plant part was used to isolate in CPG Ralstonia solanacearum media and was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (10 cfu/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method.It was observed that the isolated spp. AA2 and Results: Trichoderma PFS were most potent in inhibiting the growth of Pseudomonas fluorescence R. showing ZOI 20.67 mm and 22.33 mm, respectively. Digital solanacearum, microscopy showed distinct inhibitory effect on the growth and survival of R.The results from the field data indicated that spp. solanacearum. Trichoderma and alone were able to prevent 92% and 96% of Pseudomonas fluorescence the infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection.Results showed the bio-efficacy of the native isolates. The Conclusions: various level of antagonistic effect against shown by all 13 R. solanacearum isolates, including the results shown by native isolates in the field, manifested the promising potential of the biocontrol agents, like and Trichoderma against controlling the bacterial wilt infection. Pseudomonas

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“…An abundance of beneficial soil organisms could suppress pathogens and diseases, improve nutrient availability, promote plant growth, and thus increased the crop yield (Ji et al 2008;Ramesh et al 2009;Liu et al 2012;Yuan et al 2014). Streptomyces sp.…”

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confidence: 99%

Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system

Liu

Wang

et al. 2015

Plant Soil

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Background and aims Peanut yield and quality are seriously compromised by continuous monoculturing in the red soil region of southern China. Monoculturing can cause soil degradation and an increase in soil-borne diseases. This research aimed to investigate the influence of long-term peanut monocropping and different fertilization treatments on peanut growth, soil physical and chemical properties and soil microbial community. Methods A long-term fertilization experiment established in 1996 was utilized to examine the effect of various fertilization treatments including chemical and organic fertilizers treatments. Deep 16S rRNA gene pyrosequencing highlighted changes in the abundance and structure of bacterial communities, especially of the pathogenic and beneficial bacterial communities in long term chemical fertilizer treatment in comparison to the organic manure treatment. Results Chemical fertilizer treatment causes a shift in bacterial community structure and decrease in diversity under the long-term monocropping in comparison to organic fertilizer. The abundance of the bacterial pathogen Ralstonia solanacearum, a causative agent of peanut wilt, was found to be associated with a loss of community diversity and loss of the peanut yield. Conclusions The organic fertilizers more effectively increase microbial diversity in the soil and changed the community structure. Long-term use of the chemical fertilizer leads to a decrease in microbial diversity of the soil and an increase in R. solanacearum with associated increase of peanut wilt. The potential decrease in diversity and competition between the bacterial community and the pathogen may be a contributing factor to increased disease during long-term chemical fertilizer use.

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Biological control against bacterial wilt and colonization of mulberry by an endophytic Bacillus subtilis strain

Cited by 145 publications

References 41 publications

Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

Evaluation of Trichoderma spp., Pseudomonas fluorescence and Bacillus subtilis for biological control of Ralstonia wilt of tomato

Changes in the abundance and structure of bacterial communities under long-term fertilization treatments in a peanut monocropping system

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