Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci

He, Yubin; Li, Xiaolu; Xue, Xiaoyun; Swita, Marie; Schmidt, Andrew J.; Yang, Bin

doi:10.1016/j.biortech.2016.10.059

Cited by 45 publications

(8 citation statements)

References 32 publications

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“…Thus, biological lignin valorization is a challenging process. The bioconversion of biomass-derived lignin to value-added products by Pseudomonas putida has been recently demonstrated [11,36,37]. The bioprocessing of low-cost lignin to PHA can potentially reduce costs for both biomass biorefineries and the glycerol-to-bioplastic process.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

Pan

et al. 2021

Biotechnol Biofuels

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Background Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic responses that enable Pseudomonas putida to utilize mixed carbon sources to generate reducing power and polyhydroxyalkanoate (PHA) remain unclear. Previous research has mainly focused on different fermentation strategies, including the sequential feeding of xylose as the growth stage substrate and octanoic acid as the PHA-producing substrate, feeding glycerol as the sole carbon substrate, and co-feeding of lignin and glucose. This study developed a new strategy—co-feeding glycerol and lignin derivatives such as benzoate, vanillin, and vanillic acid in Pseudomonas putida KT2440—for the first time, which simultaneously improved both cell biomass and PHA production. Results Co-feeding lignin derivatives (i.e. benzoate, vanillin, and vanillic acid) and glycerol to P. putida KT2440 was shown for the first time to simultaneously increase cell dry weight (CDW) by 9.4–16.1% and PHA content by 29.0–63.2%, respectively, compared with feeding glycerol alone. GC–MS results revealed that the addition of lignin derivatives to glycerol decreased the distribution of long-chain monomers (C10 and C12) by 0.4–4.4% and increased the distribution of short-chain monomers (C6 and C8) by 0.8–3.5%. The 1H–13C HMBC, 1H–13C HSQC, and 1H–1H COSY NMR analysis confirmed that the PHA monomers (C6–C14) were produced when glycerol was fed to the bacteria alone or together with lignin derivatives. Moreover, investigation of the glycerol/benzoate/nitrogen ratios showed that benzoate acted as an independent factor in PHA synthesis. Furthermore, 1H, 13C and 31P NMR metabolite analysis and mass spectrometry-based quantitative proteomics measurements suggested that the addition of benzoate stimulated oxidative-stress responses, enhanced glycerol consumption, and altered the intracellular NAD+/NADH and NADPH/NADP+ ratios by up-regulating the proteins involved in energy generation and storage processes, including the Entner–Doudoroff (ED) pathway, the reductive TCA route, trehalose degradation, fatty acid β-oxidation, and PHA biosynthesis. Conclusions This work demonstrated an effective co-carbon feeding strategy to improve PHA content/yield and convert lignin derivatives into value-added products in P. putida KT2440. Co-feeding lignin break-down products with other carbon sources, such as glycerol, has been demonstrated as an efficient way to utilize biomass to increase PHA production in P. putida KT2440. Moreover, the involvement of aromatic degradation favours further lignin utilization, and the combination of proteomics and metabolomics with NMR sheds light on the metabolic and regulatory mechanisms for cellular redox balance and potential genetic targets for a higher biomass carbon conversion efficiency.

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Section: Introductionmentioning

confidence: 99%

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

Pan

et al. 2021

Biotechnol Biofuels

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“…In hydrothermal liquefaction processes, biomass is pyrolyzed to decompose the solid polymeric structure into a mixture of gas, biocrude, solid residue, and aqueous waste. Generally, this aqueous waste contains high concentrations of organic acids, alcohols, ketones, ammonia and lignin-based aromatics, which can be converted into lipids by some bacteria, along with the reduction of chemical oxygen demand (COD) [105]. Biomass gasification is another thermo-chemical process where biomass is converted to synthetic gas at a higher temperature.…”

Section: Application Of Multiple Metabolic Pathways For Lignin Valorimentioning

confidence: 99%

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Lei²,

Zhai

et al. 2019

Biotechnol Biofuels

211

119

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Lignin is the most abundant aromatic substrate on Earth and its valorization technologies are still under developed. Depolymerization and fragmentation are the predominant preparatory strategies for valorization of lignin to chemicals and fuels. However, due to the structural heterogeneity of lignin, depolymerization and fragmentation typically result in diverse product species, which require extensive separation and purification procedures to obtain target products. For lignin valorization, bacterial-based systems have attracted increasing attention because of their diverse metabolisms, which can be used to funnel multiple lignin-based compounds into specific target products. Here, recent advances in lignin valorization using bacteria are critically reviewed, including lignin-degrading bacteria that are able to degrade lignin and use lignin-associated aromatics, various associated metabolic pathways, and application of bacterial cultures for lignin valorization. This review will provide insight into the recent breakthroughs and future trends of lignin valorization based on bacterial systems.

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“…To the date, various types of biomass, such as plants, algae, etc., have been effectively converted to the liquid by means of liquefaction techniques, i.e. solvent-and hydrothermal liquefaction (HTL) [296][297][298]. In HTL, wet biomass slurries are heated (200-500 °C) under high pressure (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) to produce a mixture of products (e.g.…”

Section: Liquefactionmentioning

confidence: 99%

Biofuel Production Using Thermochemical Conversion of Heavy Metal-Contaminated Biomass (HMCB) Harvested from Phytoextraction Process

Dastyar

Raheem

et al. 2019

Chemical Engineering Journal

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Over the past few decades, bioenergy production from heavy metalcontaminated biomass (HMCB) has been drawing increasing attention from scientists in diverse disciplines and countries owing to their potential roles in addressing both energy crisis and environmental challenges. In this review, bioenergy recovery from HMCBs, i.e. contaminated plants and energy crops, using thermochemical processes (pyrolysis, gasification, combustion, and liquefaction) has been scrutinized. Furthermore, the necessity of the implementation of practical strategies towards sustainable phytoextraction and metalfree biofuels production has been critically discussed. To meet this aim, the paper firstly delivers the fundamental concepts regarding phytoremediation approach, and then, reviews

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Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci

Cited by 45 publications

References 32 publications

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products

Biofuel Production Using Thermochemical Conversion of Heavy Metal-Contaminated Biomass (HMCB) Harvested from Phytoextraction Process

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