Biological differences between FIM2 and FIM3 fimbriae of Bordetella pertussis: not just the serotype

Matczak, Soraya; Bouchez, Valérie; Leroux, Pauline; Douché, Thibaut; Collinet, Nils; Landier, Annie; Gianetto, Quentin Giai; Guillot, Sophie; Chamot‐Rooke, Julia; Hasan, Milena; Matondo, Mariette; Brisse, Sylvain; Toubiana, Julie

doi:10.1016/j.micinf.2023.105152

Cited by 4 publications

(2 citation statements)

References 66 publications

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“…In France, one vaccine frequently used for the primary schedule in infants, and some booster vaccines do not contain pertactin, which may have created an opportunity for pertactin-positive Bp isolates to spread more efficiently. We also show that FIM2 fimbriae expression is predominant, whereas nearly two thirds of Bp isolates before the COVID-19 pandemic expressed FIM3 [ 4 ], as in other countries [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 68%

Resurgence of Bordetella pertussis, including one macrolide-resistant isolate, France, 2024

Rodrigues,

Bouchez,

Soares

et al. 2024

Eurosurveillance

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As other European countries, France is experiencing a resurgence of pertussis in 2024. Between 1 January and 31 May 2024, 5,616 (24.9%) positive Bordetella pertussis qPCR tests were identified, following a 3-year period of almost null incidence. Of 67 cultured and whole genome sequenced B. pertussis isolates, 66 produced pertactin and 56 produced FIM2, in contrast to pre-COVID-19 years. One isolate of genotype Bp-AgST4 was resistant to macrolides. Pertussis resurgence may favour isolates that produce FIM2 and pertactin.

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Section: Discussionmentioning

confidence: 68%

Resurgence of Bordetella pertussis, including one macrolide-resistant isolate, France, 2024

Rodrigues,

Bouchez,

Soares

et al. 2024

Eurosurveillance

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“…We then added 4 mL of the adjusted concentration of the bacterial suspension to the test tube, let it stand for 1, 3, and 5 h at room temperature, respectively, and measured its OD 600 nm by absorbing the upper solution after standing, which was recorded as OD 2 , and measurement of each sample was repeated 3 times. According to the difference in absorbance of the bacterial solution before and after standing, the self-agglutination rate of each strain was calculated, and the formula was as follows [24]:…”

Section: Determination Of Surface Hydrophobicity Of L Monocytogenes (...mentioning

confidence: 99%

Differences in Biofilm Formation of Listeria monocytogenes and Their Effects on Virulence and Drug Resistance of Different Strains

Yang,

Kong,

Niu

et al. 2024

Foods

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Listeria monocytogenes is recognized as one of the primary pathogens responsible for foodborne illnesses. The ability of L. monocytogenes to form biofilms notably increases its resistance to antibiotics such as ampicillin and tetracycline, making it exceedingly difficult to eradicate. Residual bacteria within the processing environment can contaminate food products, thereby posing a significant risk to public health. In this study, we used crystal violet staining to assess the biofilm-forming capacity of seven L. monocytogenes strains and identified ATCC 19112 as the strain with the most potent biofilm-forming. Subsequent fluorescence microscopy observations revealed that the biofilm-forming capacity was markedly enhanced after two days of culture. Then, we investigated into the factors contributing to biofilm formation and demonstrated that strains with more robust extracellular polymer secretion and self-agglutination capabilities exhibited a more pronounced ability to form biofilms. No significant correlation was found between surface hydrophobicity and biofilm formation capability. In addition, we found that after biofilm formation, the adhesion and invasion of cells were enhanced and drug resistance increased. Therefore, we hypothesized that the formation of biofilm makes L. monocytogenes more virulent and more difficult to remove by antibiotics. Lastly, utilizing RT-PCR, we detected the expression levels of genes associated with biofilm formation, including those involved in quorum sensing (QS), flagellar synthesis, and extracellular polymer production. These genes were significantly upregulated after biofilm formation. These findings underscore the critical relationship between extracellular polymers, self-agglutination abilities, and biofilm formation. In conclusion, the establishment of biofilms not only enhances L. monocytogenes’ capacity for cell invasion and adhesion but also significantly increases its resistance to drugs, presenting a substantial threat to food safety.

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Bordetella pertussis isolates in Finland after acellular vaccination: serotype change and biofilm formation

Niinikoski,

Barkoff,

Mertsola

et al. 2024

Clinical Microbiology and Infection

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Biological differences between FIM2 and FIM3 fimbriae of Bordetella pertussis: not just the serotype

Cited by 4 publications

References 66 publications

Resurgence of Bordetella pertussis, including one macrolide-resistant isolate, France, 2024

Resurgence of Bordetella pertussis, including one macrolide-resistant isolate, France, 2024

Differences in Biofilm Formation of Listeria monocytogenes and Their Effects on Virulence and Drug Resistance of Different Strains

Bordetella pertussis isolates in Finland after acellular vaccination: serotype change and biofilm formation

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