Biological Laboratory X-ray Microscopy

Kördel, Mikael; Fogelqvist, Emelie; Carannante, Valentina; Önfelt, Björn; Reddy, Hemanth K. N.; Okamoto, Kenta; Svenda, Martin; Sellberg, Jonas A.; Hertz, Hans M.

doi:10.1017/s1431927618014022

Cited by 2 publications

(1 citation statement)

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“…This limits access to the technique, with demand outstripping available hours of experimental access. However, tremendous advances are being made in developing "tabletop" soft X-ray sources 96,97 (such as the SiriusXT source 98 ; https://siriu sxt.com), slated to become commercially available soon. As their name suggests, these new sources are sized to fit in a typical academic or industrial laboratory.…”

Section: Discussionmentioning

confidence: 99%

Soft X‐ray tomograms provide a structural basis for whole‐cell modeling

et al. 2022

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Developing in silico models that accurately reflect a whole, functional cell is an ongoing challenge in biology. Current efforts bring together mathematical models, probabilistic models, visual representations, and data to create a multi‐scale description of cellular processes. A realistic whole‐cell model requires imaging data since it provides spatial constraints and other critical cellular characteristics that are still impossible to obtain by calculation alone. This review introduces Soft X‐ray Tomography (SXT) as a powerful imaging technique to visualize and quantify the mesoscopic (~25 nm spatial scale) organelle landscape in whole cells. SXT generates three‐dimensional reconstructions of cellular ultrastructure and provides a measured structural framework for whole‐cell modeling. Combining SXT with data from disparate technologies at varying spatial resolutions provides further biochemical details and constraints for modeling cellular mechanisms. We conclude, based on the results discussed here, that SXT provides a foundational dataset for a broad spectrum of whole‐cell modeling experiments.

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Section: Discussionmentioning

confidence: 99%