Biological parameters influencing the human umbilical cord‐derived mesenchymal stem cells' response to retinoic acid

Saraee, Farnoosh; Sagha, Mohsen; Kouchesfahani, Homa Mohseni; Abdanipour, Alireza; Maleki, Masoud; Nikougoftar, Mahin

doi:10.1002/biof.1192

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…These findings propose the importance of logarithmic phase of the growth kinetics in cellular response to cytotoxic agents such as RA. This was confirmed by the telomerase activity assay in hUCMSCs at P2 found the higher telomerase activity rate than the other passages [16] .…”

Section: Research Highlightsupporting

confidence: 59%

“…In contrast to neural differentiation role of RA in previous described studies, our recently published work addressed the opposite function for RA on fetal cells that it promotes apoptosis and no differentiation on human umbilical cord -derived mesenchymal stem cells (hUCMSCs) [16] . Apoptosis is the process of programmed cell death which occurs in normal physiological condition such as cell turnover, immunity system regulation and embryogenesis.…”

Section: Research Highlightmentioning

confidence: 95%

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Opposing Biological Functions of Retinoic Acid in Normal Embryonic Development

Sagha

Mohammadzadeh-Vardin²

2015

Stem Cell Transl Invest

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Vitamin A and its metabolic derivative, retinoic acid (RA) are essential biomolecules for normal development regulation and regeneration in different organs. Too much and deficiency of vitamin A can be toxic for fetuses lead to birth defects or is associated with many congenital malformations. It has been known that RA alone or in combination with other morphogens promotes the developmental program such as neural differentiation and patterning. Here, we discussed RA role in the neural patterning of the embryonic stem cells and its function in promoting the neural differentiation in neural plate of the developing embryo through attenuating the fibroblast growth factor (FGF) signaling. By using different techniques, we also argued the opposite function of RA in inducing apoptosis in the human umbilical cord-derived mesenchymal stem cells (hUCMSCs) shown by upregulating the caspase expression. Finally, we discussed that some biological parameters including cell density and passage appeared to be involved in this cytotoxicity response.

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Section: Research Highlightsupporting

confidence: 59%

Section: Research Highlightmentioning

confidence: 95%

Opposing Biological Functions of Retinoic Acid in Normal Embryonic Development

Sagha

Mohammadzadeh-Vardin²

2015

Stem Cell Transl Invest

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“…The cytotoxicity of retinoic acid on mesenchymal stem cells derived from umbilical cord is highly dependent on the duration of treatment, status of cells, passages, time of culture, etc. [8,9].…”

Section: Resultsmentioning

confidence: 99%

Biochemical Effects of Retinoid Derivatives on Mesenchymal Stem Cells In Vitro

Vasincu¹,

Iancu²,

Cazan³

et al. 2018

Rev. Chim.

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The studies we performed targeted the effects of all-trans retinol (vitamin A) and some retinoid derivatives (including tretinoin or all-trans retinoic acid, retinyl propionate, 9-cis retinoic acid, 13-cis retinoic acid), as well as of tazarotenic acid on apoptosis of rat mesenchymal stem cells, cultured after isolation. Tazarotenic acid is considered to be relatively selective and a potent agonist for RARb and RARg and less for RARa. The same time, tazarotenic acid is not binding to RXRs (retinoid X receptors). The relevant analysis of our experimental results demonstrated that 13-cis retinoic acid was the most potent inducer of apoptosis of cultured mesenchymal stem cells of rat origin when compared to other retinoid derivatives, as follows: 13-cis retinoic acid ] 9-cis retinoic acid ] tazarotenic acid ] all-trans retinoic acid ] retinyl propionate ] retinol (or vitamin A). Very interesting and unexpected were the apoptotic effects of 1 �M tazarotenic acid for 24 hours in our experiments, very close to those induced by all-trans retinoic acid (tretinoin). The apoptosis induced by 13-cis retinoic acid, a principal activator of RARb and RARg, and that induced by 9-cis retinoic acid, a major activator of RXRs, suggests different pathways activated by these retinoid derivatives.

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