“…If this more conventionally applied bioflocs-to-AGS transition setup were applied, the external physical selector would be the only driving force to transform flocculent sludge to AGS, and thus a much more radical biofloc washout type of short SRT would have to be employed ( Sun et al., 2019 ). Even with the sidestream hydrocyclone installation, only 5–7% of the RAS can be subjective to physical selection ( Avila et al., 2021 ; Partin, 2019 ; Regmi et al., 2022 ), leaving majority of the bioflocs and AGS unseparated in the RAS returned to the feast zone where bioflocs gain more growth advantage over AGS, which explains the slow startup period, e.g., one year, needed to observe sludge settleability improvement ( Ford et al., 2016 ; Kent et al., 2018 ; Roche et al., 2022 ; Welling et al., 2015 ; Wett et al., 2015 ).…”