Biological responses induced by high molecular weight chitosan administrated jointly with Platelet-derived Growth Factors in different mammalian cell lines

Santo, Mariana Carolina Di; Alaimo, Agustina; Acebedo, Sofía L.; Spagnuolo, Carla; Pozner, Raúl; Pérez, Oscar E.

doi:10.1016/j.ijbiomac.2020.05.032

Cited by 11 publications

(8 citation statements)

References 88 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…H-CS was purified before its use according to the protocol previously described [ 16 , 20 ]. In a preceding study of our group, the viscosity-average MW of chitosan was determined (300 KDa).…”

Section: Methodsmentioning

confidence: 99%

“…Upon reached the optimal confluence, cells were treated with H-CS couple with the Cyanine (Cyn) fluorescent probe (50 μg/mL), according to Ref. [ 16 ]. After 24 h, culture media was removed, and cells were washed with PBS and fixed with 4% paraformaldehyde.…”

Section: Methodsmentioning

confidence: 99%

“…CS is a linear polysaccharide whose structure is comprised of β-1,4-linked 2-amino-2-deoxy-β- d -glucose (deacetylated d -glucosamine) and N -acetyl- d -glucosamine units [ 15 , 16 ]. The presence of –NH 2 and –OH groups in the CS structure gives it interesting chemical and biological properties.…”

Section: Introductionmentioning

confidence: 99%

“…Particularly, the biocompatibility of H-CS was analysed here. This polysaccharide was obtained from chitin of Pleoticus muelleri shrimp wastes, the main crustacean exploited in Mar del Plata, Argentine maritime platform of the Atlantic Ocean [ 16 , 20 , 21 ]. Those marine exoskeleton wastes are environmental pollutant, but these raw materials could be useful for obtaining of beneficial products for health.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Santo

Alaimo

Rubio

et al. 2020

Biochemistry and Biophysics Reports

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Santo

Alaimo

Rubio

et al. 2020

Biochemistry and Biophysics Reports

Self Cite

View full text Add to dashboard Cite

“…Platelets are known to contain high quantities of growth factors, such as transforming growth factors ß-1 (TGFß-1), platelet-derived growth factor (PDGF), epithelial growth factor (EGF), insulin growth factor-I (IFG-I) and vascular endothelial growth factors (VEGF) which may have a role in stimulating cell proliferation and up regulating angiogenesis (5) . Mineralized plasmatic matrix (MPM) is used as one of the most recent grafting materials.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mineralized Plasmatic Matrix as a grafting material around immediate dental implant in mandibular posterior teeth

Mansour

Khalil

Shuman

2021

Al-Azhar Journal of Dental Science

View full text Add to dashboard Cite

Objective: the present study was performed to evaluate Mineralized Plasmatic Matrix (MPM) as a grafting material around immediate dental implant in mandibular posterior teeth. Subjects and Methods: Eight patients were selected for immediate dental implant in mandibular posterior teeth and use MPM as a grafting material around the implant. After surgery each patient were evaluated clinically at the following interval immediate, one week, two weeks, and three months postoperatively for:: Presence of infection, Edema, Graft exposure and/or loss, Soft tissue dehiscence, Pain, Nerve injury and Implant stability, and radiographically immediate and three months postoperatively interval by CBCT for: Bone density around the dental implant and Horizontal bone loss around the dental implant. Results: All patients hadn't any infection, nerve injury, Graft exposure and /or loss, soft tissue dehiscence and facial edema throughout the study till three months. Osstel at 3 months (81.0±7.68) was higher than immediately (63.40±4.16). Bone density at 3 months (447.0± 28.64) was higher than immediately (243.0±17.18). Patients experienced mild to moderate pain after surgery, at the surgical sites which decrease gradually during four days postoperatively then disappeared completely at the 7 th day. Conclusion: The use of MPM with immediate implant reduces postoperative infection, edema, pain, graft exposure and/or loss and soft tissue dehiscence. Use of MPM with immediate implant enhances the implant stability, improves bone density around it and decrease marginal bone loss around the immediate implant.

show abstract

Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

Atila,

Dalgic,

Krzemińska

et al. 2024

Adv Healthcare Materials

View full text Add to dashboard Cite

An injectable hydrogel formulation was developed utilizing low‐ and high‐molecular‐weight chitosan (LCH and HCH) incorporated with curcumin and α‐tocopherol‐loaded liposomes (Lip/Cur+Toc). Cur and Toc releases were delayed within the hydrogels. The injectability of hydrogels was proved via rheological analyses. In vitro studies were conducted using human dental pulp stem cells (hDPSCs) and human gingival fibroblasts (hGFs) to examine the biological performance of the hydrogels towards endodontics and periodontics, respectively. The viability of hDPSCs treated with the hydrogels with Lip/Cur+Toc was the highest till day 14, compared to the neat hydrogels. During odontogenic differentiation tests, ALP activity of hDPSCs was induced in the Cur‐containing groups. Biomineralization was enhanced mostly with Lip/Cur+Toc incorporation. The viability of hGFs was the highest in HCH combined with Lip/Cur+Toc while wound healing occurred almost 100% in both (Lip/Cur+Toc@LCH and Lip/Cur+Toc@HCH) after 2 days. Antioxidant activity of Lip/Cur+Toc@LCH on hGFs was significantly the highest among the groups. Antimicrobial tests demonstrated that Lip/Cur+Toc@LCH was more effective against Escherichia coli whereas so was Lip/Cur+Toc@HCH against Staphylococcus aureus. The antimicrobial mechanism of the hydrogels was investigated for the first time through various computational models. LCH and HCH loaded with Lip/Cur+Toc were promising candidates with multi‐functional features for endodontics and periodontics.This article is protected by copyright. All rights reserved

show abstract

Biological responses induced by high molecular weight chitosan administrated jointly with Platelet-derived Growth Factors in different mammalian cell lines

Cited by 11 publications

References 88 publications

Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

Evaluation of Mineralized Plasmatic Matrix as a grafting material around immediate dental implant in mandibular posterior teeth

Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

Contact Info

Product

Resources

About